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Confocal laser scanning microscopy

Confocal laser scanning microscopy (CLSM or LSCM) is a valuable tool for obtaining high resolution images and 3-D reconstructions.

The key feature of confocal microscopy is its ability to produce blur-free images of thick specimens at various depths.

Images are taken point-by-point and reconstructed with a computer, rather than projected through an eyepiece.

The principle for this special kind of microscopy was developed by Marvin Minsky in 1953, but it took another thirty years and the development of lasers for confocal microscopy to become a standard technique toward the end of the 1980s.

Note: This article excerpts material from the Wikipedia article "Confocal laser scanning microscopy", which is released under the GNU Free Documentation License.

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