Featured Research

from universities, journals, and other organizations

Researchers replicate human kidney gene changes in mouse model

Date:
May 23, 2011
Source:
University of Louisville
Summary:
Researchers have replicated the inflammatory gene changes of a human kidney as it progresses from mild to severe diabetic nephropathy, using a mouse model, according to a new article.

University of Louisville researchers have replicated the inflammatory gene changes of a human kidney as it progresses from mild to severe diabetic nephropathy, using a mouse model developed by a UofL researcher, according to an article published May 23 in the journal Experimental Nephrology (merged with Nephron Experimental Nephrology). Diabetic nephropathy is the foremost cause of kidney failure.

"In 2004 we published an article that showed that our diabetic mouse model, OVE26, excreted high levels of protein in the urine, as humans with diabetes do. We continue to see resemblance to human diabetes as we test different aspects of the disease using this mouse model," said Paul Epstein, Ph.D., acting director of the Kosair Children's Hospital Research Institute.

The processes leading to advanced diabetic nephropathy are poorly understood. Researchers have not been able to observe kidney failure through its full cycle of development, because of the natural lifespan and other limitations of available diabetic animal models.

Because of these limitations, changes in renal gene expression can be used to evaluate the progression of diabetic nephropathy. Gene expression studies, which measure the gene's synthesis of messenger RNA, identify individual genes or major signaling pathways activated as diabetic nephropathy progresses.

This is the first study of gene expression changes of whole kidney during the progression from mild to very severe albuminuria, a condition common to patients with longstanding diabetes.

Researchers found that some gene expression differences between control and diabetic mice increased 10-fold. The change was most obvious for inflammatory genes.

This suggests that this strain of diabetic mice could be used to look for new insights into human diabetic nephropathy and raises questions about the role of inflammation in kidney failure.

"They provide an excellent model of diabetic nephropathy to assess the effect of inflammatory proteins," Epstein said. "In future studies, we can use this mouse model to explore whether inflammation causes disease progression or if the progression of the disease causes further inflammation. If it turns out that inflammation is causal, the next step would be to test the effectiveness of anti-inflammatory drugs."


Story Source:

The above story is based on materials provided by University of Louisville. Note: Materials may be edited for content and length.


Journal Reference:

  1. yang, L., Brozovic, S., Xu, J., Long, Y., Kralik, P.M., Waigel, S., Zacharias, W., Zheng, S., Epstein, P.N. Inflammatory Gene Expression in OVE26 Diabetic Kidney during the Development of Nephropathy. Nephron Exp Nephrol, 2011;119:e8-e20 DOI: 10.1159/000324407

Cite This Page:

University of Louisville. "Researchers replicate human kidney gene changes in mouse model." ScienceDaily. ScienceDaily, 23 May 2011. <www.sciencedaily.com/releases/2011/05/110523124358.htm>.
University of Louisville. (2011, May 23). Researchers replicate human kidney gene changes in mouse model. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2011/05/110523124358.htm
University of Louisville. "Researchers replicate human kidney gene changes in mouse model." ScienceDaily. www.sciencedaily.com/releases/2011/05/110523124358.htm (accessed July 24, 2014).

Share This




More Health & Medicine News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Painkiller Designed To Discourage Abuse: Will It Work?

New Painkiller Designed To Discourage Abuse: Will It Work?

Newsy (July 24, 2014) The FDA approved Targiniq ER on Wednesday, a painkiller designed to keep users from abusing it. Like any new medication, however, it has doubters. Video provided by Newsy
Powered by NewsLook.com
Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Doctor At Forefront Of Fighting Ebola Outbreak Gets Ebola

Newsy (July 24, 2014) Sheik Umar Khan has treated many of the people infected in the Ebola outbreak, and now he's become one of them. Video provided by Newsy
Powered by NewsLook.com
Condemned Man's US Execution Takes Nearly Two Hours

Condemned Man's US Execution Takes Nearly Two Hours

AFP (July 24, 2014) America's death penalty debate raged Thursday after it took nearly two hours for Arizona to execute a prisoner who lost a Supreme Court battle challenging the experimental lethal drug cocktail. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com
China's Ageing Millions Look Forward to Bleak Future

China's Ageing Millions Look Forward to Bleak Future

AFP (July 24, 2014) China's elderly population is expanding so quickly that children struggle to look after them, pushing them to do something unexpected in Chinese society- move their parents into a nursing home. Duration: 02:07 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins