Featured Research

from universities, journals, and other organizations

Promising new method for next-generation live-attenuated viral vaccines against Chikungunya virus

Date:
February 21, 2013
Source:
Public Library of Science
Summary:
Researchers have applied a novel method of vaccine creation for Chikungunya virus using a technique called large scale random codon re-encoding. Using this approach, scientists have demonstrated that the engineered viruses exhibit a stable phenotype with a significantly decreased viral fitness, making it a new vaccine candidate for this emerging viral disease.

Researchers have successfully applied a novel method of vaccine creation for Chikungunya virus (CHIKV) using a technique called large scale random codon re-encoding. Using this approach, a group from the UMR_D 190, Emerging viruses Department in Marseille, France in collaboration with the University of Sydney, Australia, demonstrated that the engineered viruses exhibit a stable phenotype with a significantly decreased viral fitness (i.e., replication capacity), making it a new vaccine candidate for this emerging viral disease.

This new report publishes on February 21 in the Open Access journal, PLOS Pathogens.

There is an immense need for the development of vaccines targeting many emerging viral pathogens. CHIKV has been responsible for several million human cases over the last decade and represents a striking example of a re-emerging, arthropod-borne, human pathogen for which no licensed vaccine exists. Worryingly, one of the vectors of CHIKV, the mosquito Aedes albopictus, has dispersed into new regions (including temperate areas) resulting in outbreaks of this disease where they had never been previously observed, for example in Italy.

Using the large-scale codon re-encoding method, Antoine Nougairede and colleagues were able to synthetically modify the nucleic acid composition of the virus without modifying the encoded viral proteins. When this method was applied to poliovirus and Influenza A virus, it resulted in a live but attenuated virus that had significant reduction of viral fitness. In contrast with previous studies, which employed a targeted approach of codon re-encoding, this new study demonstrates that a random approach reduced the replicative fitness of CHIKV in both primate and arthropod cells. The employed strategy also prevented the reversion of the attenuated phenotype by mutation or recombination, thus reducing the possibility that the newly created virus strain could evolve back to the pathogenic version.

The findings by Nougairede et al. suggest that large-scale codon re-encoding can provide a strong basis for the rapid design of next-generation viral vaccines against emerging viral pathogens, as soon as their genome sequence has been determined. It represents an exciting route to vaccine development because it intrinsically alleviates the likelihood of novel pathogenic properties of the designed live vaccine, and allows modulation of the amount of reduced fitness by altering the terms and degree of the genetic re-encoding. Thus, this strategy potentially allows for the generic development of live attenuated vaccines against many new viral pathogens, with reduced costs and the potential single dose induction of long-term immunity.


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Antoine Nougairede, Lauriane De Fabritus, Fabien Aubry, Ernest A. Gould, Edward C. Holmes, Xavier de Lamballerie. Random Codon Re-encoding Induces Stable Reduction of Replicative Fitness of Chikungunya Virus in Primate and Mosquito Cells. PLoS Pathogens, 2013; 9 (2): e1003172 DOI: 10.1371/journal.ppat.1003172

Cite This Page:

Public Library of Science. "Promising new method for next-generation live-attenuated viral vaccines against Chikungunya virus." ScienceDaily. ScienceDaily, 21 February 2013. <www.sciencedaily.com/releases/2013/02/130221194235.htm>.
Public Library of Science. (2013, February 21). Promising new method for next-generation live-attenuated viral vaccines against Chikungunya virus. ScienceDaily. Retrieved August 20, 2014 from www.sciencedaily.com/releases/2013/02/130221194235.htm
Public Library of Science. "Promising new method for next-generation live-attenuated viral vaccines against Chikungunya virus." ScienceDaily. www.sciencedaily.com/releases/2013/02/130221194235.htm (accessed August 20, 2014).

Share This




More Plants & Animals News

Wednesday, August 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Possible Ebola Patient in Isolation at California Hospital

Possible Ebola Patient in Isolation at California Hospital

Reuters - US Online Video (Aug. 20, 2014) — A patient who may have been exposed to the Ebola virus is in isolation at the Kaiser Permanente South Sacramento Medical Center. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Flower Power! Dandelions Make Car Tires?

Flower Power! Dandelions Make Car Tires?

Reuters - Business Video Online (Aug. 20, 2014) — Forget rolling on rubber, could car drivers soon be traveling on tires made from dandelions? Teams of scientists are racing to breed a type of the yellow flower whose taproot has a milky fluid with tire-grade rubber particles in it. As Joanna Partridge reports, global tire makers are investing millions in research into a new tire source. Video provided by Reuters
Powered by NewsLook.com
Unsustainable Elephant Poaching Killed 100K In 3 Years

Unsustainable Elephant Poaching Killed 100K In 3 Years

Newsy (Aug. 20, 2014) — Poachers have killed 100,000 elephants between 2010 and 2012, as the booming ivory trade takes its toll on the animals in Africa. Video provided by Newsy
Powered by NewsLook.com
Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Newsy (Aug. 19, 2014) — Scientists have developed a new device that mimics the way octopuses blend in with their surroundings to hide from dangerous predators. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins