Featured Research

from universities, journals, and other organizations

3-D stem cell culture technique developed to better understand Alzheimer's disease

Date:
April 2, 2013
Source:
New York Stem Cell Foundation
Summary:
A team of researchers has developed a technique to produce three-dimensional cultures of induced pluripotent stem cells called embryoid bodies, amenable to live cell imaging and to electrical activity measurement.

A team of researchers at The New York Stem Cell Foundation Research Institute led by Scott Noggle, PhD, Director of the NYSCF Laboratory and the NYSCF -- Charles Evans Senior Research Fellow for Alzheimer's Disease, and Michael W. Nestor, PhD, a NYSCF Postdoctoral Research Fellow, has developed a technique to produce three-dimensional cultures of induced pluripotent stem (iPS) cells called embryoid bodies, amenable to live cell imaging and to electrical activity measurement.

Related Articles


As reported in their Stem Cell Research study, these cell aggregates enable scientists to both model and to study diseases such as Alzheimer's and Parkinson's disease.

The NYSCF Alzheimer's disease research team aims to better understand and to find treatments to this disease through stem cell research. For such disorders in which neurons misfire or degenerate, the NYSCF team creates "disease in a dish" models by reprogramming patients' skin and or blood samples into induced pluripotent stem (iPS) cells that can become neurons and the other brain cells affected in the diseases.

The cells in our body form three-dimensional networks, essential to tissue function and overall health; however, previous techniques to form complex brain tissue resulted in structures that, while similar in form to naturally occurring neurons, undermined imaging or electrical recording attempts.

In the current study, Noggle and Nestor with NYSCF scientists specially adapted two-dimensional culture methods to grow three-dimensional neuron structures from iPS cells. The resultant neurons were "thinned-out," enabling calcium-imaging studies, which measure the electrical activity of cells like neurons.

"Combining the advantages of iPS cells grown in a 3D environment with those of a 2D system, our technique produces cells that can be used to observe electrical activity of putative networks of biologically active neurons, while simultaneously imaging them," said Nestor. "This is key to modeling and studying neurodegenerative diseases."

Neural networks, thought to underlie learning and memory, become disrupted in Alzheimer's disease. By generating aggregates from iPS cells and comparing these to an actual patient's brain tissue, scientists may uncover how disease interferes with these cell-to-cell interactions and understand how to intervene to slow or stop Alzheimer's disease.

"This critical new tool developed by our Alzheimer's team will accelerate Alzheimer's research, enabling more accurate manipulation of cells to find a cure to this disease," said Susan L. Solomon, CEO of NYSCF.


Story Source:

The above story is based on materials provided by New York Stem Cell Foundation. Note: Materials may be edited for content and length.


Journal Reference:

  1. Michael W. Nestor, Daniel Paull, Samson Jacob, Andrew A. Sproul, Abdulaziz Alsaffar, Brian A. Campos, Scott A. Noggle. Differentiation of serum-free embryoid bodies from human induced pluripotent stem cells into networks. Stem Cell Research, 2013; 10 (3): 454 DOI: 10.1016/j.scr.2013.02.001

Cite This Page:

New York Stem Cell Foundation. "3-D stem cell culture technique developed to better understand Alzheimer's disease." ScienceDaily. ScienceDaily, 2 April 2013. <www.sciencedaily.com/releases/2013/04/130402091656.htm>.
New York Stem Cell Foundation. (2013, April 2). 3-D stem cell culture technique developed to better understand Alzheimer's disease. ScienceDaily. Retrieved November 1, 2014 from www.sciencedaily.com/releases/2013/04/130402091656.htm
New York Stem Cell Foundation. "3-D stem cell culture technique developed to better understand Alzheimer's disease." ScienceDaily. www.sciencedaily.com/releases/2013/04/130402091656.htm (accessed November 1, 2014).

Share This



More Health & Medicine News

Saturday, November 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Melafind: Spotting Melanoma Without a Biopsy

Melafind: Spotting Melanoma Without a Biopsy

Ivanhoe (Oct. 31, 2014) The MelaFind device is a pain-free way to check suspicious moles for melanoma, without the need for a biopsy. Video provided by Ivanhoe
Powered by NewsLook.com
Battling Multiple Myeloma

Battling Multiple Myeloma

Ivanhoe (Oct. 31, 2014) The answer isn’t always found in new drugs – repurposing an ‘old’ drug that could mean better multiple myeloma treatment, and hope. Video provided by Ivanhoe
Powered by NewsLook.com
Chronic Inflammation and Prostate Cancer

Chronic Inflammation and Prostate Cancer

Ivanhoe (Oct. 31, 2014) New information that is linking chronic inflammation in the prostate and prostate cancer, which may help doctors and patients prevent cancer in the future. Video provided by Ivanhoe
Powered by NewsLook.com
Sickle Cell: Stopping Kids’ Silent Strokes

Sickle Cell: Stopping Kids’ Silent Strokes

Ivanhoe (Oct. 31, 2014) Blood transfusions are proving crucial to young sickle cell patients by helping prevent strokes, even when there is no outward sign of brain injury. Video provided by Ivanhoe
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins