Featured Research

from universities, journals, and other organizations

Over-produced autism gene alters synapses, affects learning and behavior in mice

Date:
June 5, 2013
Source:
University of Oregon
Summary:
A gene linked to autism spectrum disorders that was manipulated in two lines of transgenic mice produced mature adults with irreversible deficits affecting either learning or social interaction.

A gene linked to autism spectrum disorders that was manipulated in two lines of transgenic mice produced mature adults with irreversible deficits affecting either learning or social interaction.

The findings, published in the May 29 issue of the Journal of Neuroscience, have implications for potential gene therapies but they also suggest that there may be narrow windows of opportunity to be effective, says principal investigator Philip Washbourne, a professor of biology and member of the University of Oregon's Institute of Neuroscience.

The research, reported by an 11-member team from three universities, targeted the impacts of alterations in the gene neuroligin 1 -- one of many genes implicated in human autism spectrum disorders -- to neuronal synapses in the altered mice during postnatal development and as they entered adulthood. One group over-expressed the normal gene, the other a mutated version.

Mice with higher-than-normal levels of the normal gene after a month had skewed synapses at maturity. Many were larger, appearing more mature, than normal. In these mice, Washbourne said, there were clear cognitive problems. "Behavior was just not normal. They didn't learn very well, and they were slower to learn, but their social behavior was not impacted."

Mice over-producing a mutated version of the gene reached adulthood with structurally immature synapses. "They were held back in development and behavior -- the way they behave in terms of learning and memory, in terms of social interaction," he said. "These were adult mice, three months old, but they behaved like normal mice at four weeks old. We saw arrested development. Learning is a little bit better, they are more flexible just like young mice, they learn faster, but their social interaction is off. To us, this looked more like Asperger's syndrome.

"So with the same gene, doing two different manipulations -- overexpressing the normal form or overexpressing a mutated form -- we've gone to two different ends of the autism spectrum," said Washbourne, whose lab focuses on basic synapse formation and what goes wrong in relationship to autism. Work has been done in both mice and zebra fish.

"We made these mice so that we can turn the genes on and off as we want," Washbourne said. "Using an antibiotic, doxycycline, it turns off these altered genes that we inserted into their chromosomes. While on doxycycline, the mice are absolutely normal."

However, if the inserted gene was turned off after the completion of development, mice still showed altered synapses and behavior. This result suggests that any kind of gene therapy may have to be applied to individuals with autism early on.

Effects seen in the social behavior of mice with the mutated gene, he said, are not unlike observations reported by parents of many autistic children. While normal mice prefer to engage with new mice entering their world rather than familiar others, or even a new inanimate object, these mice split their time equally. "It's not a deficit in memory regarding which mouse is which, it's more a weighting of their interaction. Does that mean they are autistic? I don't know, but if you talk to parents of autistic children, one of the frustrating things they report is that their children treat complete strangers in exactly the same way that they treat them."

While the findings provide new insights, Washbourne said, any translation into treatment could be decades away. "A problem with autism is there are many different genes potentially involved. It could be that some day, if you are diagnosed with autism, a mouth swab might allow for the identification of the exact gene that is mutated and allow for targeted therapy," he said. "Genome sequencing already has turned up subtle mutations in lots of genes. Autism might be like cancer, with hundreds of potential combinations of faulty genes."

Co-authors with Washbourne were seven UO colleagues: doctoral students Jennifer L. Hoy, Michael Kyweriga and Lawrence Davis; postdoctoral researcher John R. L. Constable; undergraduate students Renee J. Arias and Raluca McCallum; and Michael Wehr, professor of psychology. Outside collaborators were Paola A. Haeger and Pablo E. Castillo, both of the Albert Einstein College of Medicine in New York, and Eric Schnell of the Portland VA Medical Center and Oregon Health and Science University in Portland.

"This research by Dr. Washbourne and his team has promise in the identification of potential targets for new treatments for those affected by autism spectrum disorders," said Kimberly Andrews Espy, vice president for research and innovation and dean of the UO graduate school. "Decades after initiating the basic science model for cracking the genetic code, researchers at the University of Oregon continue to do groundbreaking research that promises to improve the health and well-being of people throughout the world."

Washbourne was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health (grant R01 NS065795) and Autism Speaks, a science and advocacy organization founded in 2005 and dedicated to funding research into the causes, prevention, treatments and a cure for autism. Additional support included a grant (R01 MH081935) to Castillo from the NIH National Institute of Mental Health, a grant (DPN T32 MH18882-22) from the American Psychological Association to Hoy, a graduate-teaching fellowship to Davis from the National Science Foundation, and support to Hoy and Davis from the UO's Oregon Center for Optics. Haeger was supported by a PEW Latin American Fellow award and a Chile Bicentennial Scholarship (Becas Chile).


Story Source:

The above story is based on materials provided by University of Oregon. Note: Materials may be edited for content and length.


Journal Reference:

  1. J. L. Hoy, P. A. Haeger, J. R. L. Constable, R. J. Arias, R. McCallum, M. Kyweriga, L. Davis, E. Schnell, M. Wehr, P. E. Castillo, P. Washbourne. Neuroligin1 Drives Synaptic and Behavioral Maturation through Intracellular Interactions. Journal of Neuroscience, 2013; 33 (22): 9364 DOI: 10.1523/JNEUROSCI.4660-12.2013

Cite This Page:

University of Oregon. "Over-produced autism gene alters synapses, affects learning and behavior in mice." ScienceDaily. ScienceDaily, 5 June 2013. <www.sciencedaily.com/releases/2013/06/130605144422.htm>.
University of Oregon. (2013, June 5). Over-produced autism gene alters synapses, affects learning and behavior in mice. ScienceDaily. Retrieved April 24, 2014 from www.sciencedaily.com/releases/2013/06/130605144422.htm
University of Oregon. "Over-produced autism gene alters synapses, affects learning and behavior in mice." ScienceDaily. www.sciencedaily.com/releases/2013/06/130605144422.htm (accessed April 24, 2014).

Share This



More Mind & Brain News

Thursday, April 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Study Says Most Crime Not Linked To Mental Illness

Study Says Most Crime Not Linked To Mental Illness

Newsy (Apr. 22, 2014) A new study finds most crimes committed by people with mental illness are not caused by symptoms of their illness or disorder. Video provided by Newsy
Powered by NewsLook.com
How Smaller Plates And Cutlery Could Make You Feel Fuller

How Smaller Plates And Cutlery Could Make You Feel Fuller

Newsy (Apr. 22, 2014) NBC's "Today" conducted an experiment to see if changing the size of plates and utensils affects the amount individuals eat. Video provided by Newsy
Powered by NewsLook.com
Do We Get Nicer With Age?

Do We Get Nicer With Age?

Newsy (Apr. 22, 2014) A recent report claims personality can change over time as we age, and usually that means becoming nicer and more emotionally stable. Video provided by Newsy
Powered by NewsLook.com
How to Master Motherhood With the Best Work/Life Balance

How to Master Motherhood With the Best Work/Life Balance

TheStreet (Apr. 22, 2014) In the U.S., there are more than 11 million couples trying to conceive at any given time. From helping celebrity moms like Bethanny Frankel to ordinary soon-to-be-moms, TV personality and parenting expert, Rosie Pope, gives you the inside scoop on mastering motherhood. London-born entrepreneur Pope is the creative force behind Rosie Pope Maternity and MomPrep. She explains why being an entrepreneur offers the best life balance for her and tips for all types of moms. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins