Featured Research

from universities, journals, and other organizations

Could sticky cells cause drug resistance in chronic blood cancer?

Date:
March 10, 2014
Source:
Manchester University
Summary:
Chronic myeloid leukaemia (CML) is associated with a specific genetic mutation that results from DNA on different chromosomes breaking off and swapping places. This disrupts genes at the breakpoint and the rejoining point. One of these disrupted genes is called BCR-ABL and drugs that target this mutation have transformed the treatment of CML. However, patients can develop resistance to these drugs, which causes their cancer to return. Now scientists have measured the levels of the BCR-ABL genetic mutation in a cell model of CML. In particular, they separated cells into those that stuck to plastic – “sticky”, or adherent, cells – and those non-adherent ones that did not, and looked for differences between the two groups of cells.

Researchers in Manchester have investigated the stickiness of leukemia cells, and whether this is linked to drug resistance.

Related Articles


Chronic myeloid leukemia (CML) is associated with a specific genetic mutation that results from DNA on different chromosomes breaking off and swapping places. This disrupts genes at the breakpoint and the rejoining point. One of these disrupted genes is called BCR-ABL and drugs that target this mutation have transformed the treatment of CML. However, patients can develop resistance to these drugs, which causes their cancer to return.

Now scientists from The University of Manchester -- part of the Manchester Cancer Research Centre -- have measured the levels of the BCR-ABL genetic mutation in a cell model of CML. In particular, they separated cells into those that stuck to plastic -- "sticky," or adherent, cells -- and those non-adherent ones that did not, and looked for differences between the two groups of cells.

Dr Richard Byers, who jointly led the research, said: "Previous studies have linked high levels of the BCR-ABL mutation with drug resistance. We wanted to see how expression of BCR-ABL differed across groups of CML cells, and in particular whether there were differences between adherent and non-adherent populations."

In a study recently published in the journal Experimental Haematology, the group demonstrate that is wide variation in BCR-ABL expression levels in the cell model and the stickier cells have a higher level of BCR-ABL expression. These stickier cells were less sensitive to treatment with a BCR-ABL targeted drug, imatinib.

Dr Byers said: "The small number of cells that show high levels of BCR-ABL may not be detectable through bulk analysis of large samples.

"It looks like it is important to look at protein levels in single cells. In future, it may be possible to measure BCR-ABL levels in individual cells in the clinic -- this will help us identify the resistant high BCR-ABL cells and better understand how patients develop resistance to imatinib treatment with the aim of combatting this resistance to make response more durable and the treatment more effective."


Story Source:

The above story is based on materials provided by Manchester University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ehsan Ghayoor Karimiani, Fiona Marriage, Anita J. Merritt, John Burthem, Richard John Byers, Philip J.R. Day. Single-cell analysis of K562 cells: An imatinib-resistant subpopulation is adherent and has upregulated expression of BCR-ABL mRNA and protein. Experimental Hematology, 2013; DOI: 10.1016/j.exphem.2013.11.006

Cite This Page:

Manchester University. "Could sticky cells cause drug resistance in chronic blood cancer?." ScienceDaily. ScienceDaily, 10 March 2014. <www.sciencedaily.com/releases/2014/03/140310090751.htm>.
Manchester University. (2014, March 10). Could sticky cells cause drug resistance in chronic blood cancer?. ScienceDaily. Retrieved March 5, 2015 from www.sciencedaily.com/releases/2014/03/140310090751.htm
Manchester University. "Could sticky cells cause drug resistance in chronic blood cancer?." ScienceDaily. www.sciencedaily.com/releases/2014/03/140310090751.htm (accessed March 5, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, March 5, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Bupa Eyes India Healthcare Opportunities

Bupa Eyes India Healthcare Opportunities

Reuters - Business Video Online (Mar. 5, 2015) — Bupa is hoping to expand in India&apos;s fast-growing health insurance market, once a rule change on foreign investment is implemented. The British private healthcare group&apos;s CEO tells Grace Pascoe why it&apos;s so keen on the new opportunity. Video provided by Reuters
Powered by NewsLook.com
Doctor in Your Pocket Is Getting Smarter

Doctor in Your Pocket Is Getting Smarter

Reuters - Business Video Online (Mar. 5, 2015) — Mobile apps are turning smartphones into a personal doctors, with users able to measure heart rate, blood pressure and even blood sugar. But will it change our behaviour? Ivor Bennett reports from the Mobile World Congress in Barcelona. Video provided by Reuters
Powered by NewsLook.com
AbbVie Inks $21B Deal To Buy Cancer Drugmaker Pharmacyclics

AbbVie Inks $21B Deal To Buy Cancer Drugmaker Pharmacyclics

Newsy (Mar. 5, 2015) — AbbVie announced Wednesday it will buy cancer drugmaker Pharmacyclics in a $21 billion deal. Video provided by Newsy
Powered by NewsLook.com
Adults Only Get The Flu Twice A Decade, Researchers Say

Adults Only Get The Flu Twice A Decade, Researchers Say

Newsy (Mar. 4, 2015) — Researchers found adults only get the flu about once every five years. Scientists analyzed how a person&apos;s immunity builds up over time as well. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins