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Metabolic mystery solved, lending insight into Lafora disease

Date:
January 26, 2015
Source:
University of Kentucky
Summary:
The metabolic function of the essential enzyme laforin has been identified by researchers, which opens new pathways to treating the deadly Lafora's disease. Lafora disease occurs as a result of the laforin gene being mutated. Mutations in the gene encoding the laforin protein result in the accumulation aberrant glycogen-like accumulations called Lafora bodies that resemble plant starch more than human glycogen.
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Scientists at the University of Kentucky College of Medicine have determined how an enzyme essential for energy metabolism functions, solving a mystery eluding molecular biologists for decades.

Matthew Gentry, Ph.D, and Craig Vander Kooi, Ph.D, associate professors of molecular and cellular biochemistry, and researcher Madushi Raththagala, Ph.D, recently discovered the role of the enzyme laforin in modifying human glycogen and thereby preventing neurodegeneration. Their work provides fundamental insights that link energy metabolism with the fatal, neurodegenerative form of epilepsy called Lafora disease. These findings were reported Jan. 22, 2015, in the journal, Molecular Cell.

Lafora disease was first described by Gonzalo Rodriquez-Lafora in 1911. In 1998, a team of scientists at University of Toronto identified the laforin gene as being mutated in Lafora disease patients. Mutations in the gene encoding the laforin protein result in the accumulation aberrant glycogen-like accumulations called Lafora bodies that resemble plant starch more than human glycogen.

Previous research showed Lafora bodies are the driving agents of Lafora disease. Lafora disease patients develop normally until their second decade of life when they experience an epileptic episode. These episodes increase in severity and number until the patient eventually dies from a massive seizure, status epilepticus or aspiration pneumonia.

Through their research, Raththagala, Vander Kooi, and Gentry determined the structural basis for the specific enzymatic function of laforin. This discovery allowed them to define how laforin modifies glycogen to inhibit Lafora body formation. Additionally, their work defines why specific patient mutations in laforin result in the disease, which opens pathways to understanding and treating the disease.

"A number of groups have tried to determine the structure of laforin over the last 17 years and no one had succeeded," Gentry said. "Dr. Raththagala worked tirelessly trying different strategies that we developed to achieve this goal. When we realized our success to get over the final hurdle, I had to sit down to fully comprehend our accomplishment. It is exciting to report this structure, but even more exciting to now consider potential therapeutic possibilities."


Story Source:

Materials provided by University of Kentucky. Original written by Elizabeth Adams. Note: Content may be edited for style and length.


Journal Reference:

  1. Madushi Raththagala, M. Kathryn Brewer, Matthew W. Parker, Amanda R. Sherwood, Brian K. Wong, Simon Hsu, Travis M. Bridges, Bradley C. Paasch, Lance M. Hellman, Satrio Husodo, David A. Meekins, Adam O. Taylor, Benjamin D. Turner, Kyle D. Auger, Vikas V. Dukhande, Srinivas Chakravarthy, Pascual Sanz, Virgil L. Woods, Sheng Li, Craig W. Vander Kooi, Matthew S. Gentry. Structural Mechanism of Laforin Function in Glycogen Dephosphorylation and Lafora Disease. Molecular Cell, 2015; 57 (2): 261 DOI: 10.1016/j.molcel.2014.11.020

Cite This Page:

University of Kentucky. "Metabolic mystery solved, lending insight into Lafora disease." ScienceDaily. ScienceDaily, 26 January 2015. <www.sciencedaily.com/releases/2015/01/150126124441.htm>.
University of Kentucky. (2015, January 26). Metabolic mystery solved, lending insight into Lafora disease. ScienceDaily. Retrieved March 28, 2024 from www.sciencedaily.com/releases/2015/01/150126124441.htm
University of Kentucky. "Metabolic mystery solved, lending insight into Lafora disease." ScienceDaily. www.sciencedaily.com/releases/2015/01/150126124441.htm (accessed March 28, 2024).

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