Featured Research

from universities, journals, and other organizations

Researchers Find Multiple Proteins That Stick To Medical Devices

Date:
October 6, 2005
Source:
Washington University in St. Louis
Summary:
Biomedical engineers at Washington University in St. Louis have found a new role for the blood protein serum amyloid P in the body's response to medical materials, which may help to explain a variety of problems associated with heart-lung bypass, hemodialysis and the use of artificial vascular grafts.

Donald Elbert (right) working with his graduate student Evan Scott at the optical waveguide light spectroscope to observe proteins sticking to a polymer surface in their Whitaker Building laboratory.
Credit: Photo by David Kilper / WUSTL Photo

Related Articles


Using atechnique called proteomics, the researchers identified many of theblood proteins that stick (adsorb) to the surfaces of medical devices.Blood proteins that adsorb to the surfaces of materials unfold and canbe recognized by the body, which then mounts a response against thedevice. The body's response to adsorbed proteins contributes to avariety of problems, including the formation of small clots that mayclose off small diameter vascular grafts or break away to end up in thelungs, kidney or brain.

Previously, the study of blood proteinson the surfaces of medical devices has been limited by the large numberof unique proteins in the blood -- greater than 150 -- as well as theextremely small amounts of proteins adsorbed on the materials. Forexample, the amount of protein that might adsorb to one side of aquarter is about a millionth of a gram.

Donald Elbert, Ph.D.,Washington University assistant professor of biomedical engineering,used advanced protein separations and mass spectrometry to track theproteins on the surfaces of various polymers used in medical devices.The analysis techniques, collectively called 'proteomics,' are mostoften used to study protein expression in cells.

"The techniquesare extremely sensitive and are really well-suited to studying proteinson surfaces," said Elbert. "Using these techniques, we can in principleidentify a protein given only a billionth of a gram of the protein,even if the protein were mixed with many other types of proteins."

Elbertand his colleagues Jinku Kim and Evan Scott were able to follow theadsorption of multiple blood proteins on the surface of a biomaterialover time.

"Traditionally, most studies were limited to the 'big three' proteins in blood - albumin, fibrinogen and IgG", Elbert said.

The results were published in the Oct. 1, 2005 issue of the Journal of Biomedical Materials Research.

New role for serum amyloid P

Bycasting a wider net, they found one particular protein on the materialsin large amounts, serum amyloid P. Serum amyloid P is very similar instructure to C-reactive protein, a well-known marker for cardiovasculardisease. Normally, serum amyloid P is involved in the clearance of DNAthat is released from dying cells, protecting the individual fromauto-immune disorders.

"No one had ever observed serum amyloid Pon biomedical materials before, because, unless you were specificallylooking for them, the technology wasn't around to easily identifyproteins present in such small amounts," Elbert said. "No one,including us, had ever posed the hypothesis that this protein might beimportant in the biocompatibility of materials. Our results show theimportance of large-scale techniques that emphasize discovery of newknowledge, rather than just hypothesis-testing."

The WashingtonUniversity researchers also found that leukocytes -- white blood cells-- adhered to the serum amyloid P after it adsorbed to surface.Leukocyte adhesion and activation on biomaterials is an important partof the body's response to medical devices. For example, large numbersof activated leukocytes are found stuck to heart-lung bypass machines,and these cells can activate blood clotting. This in turn maycontribute to neurocognitive impairment following the use of thesedevices, possibly due to small clots that break away from the device.

"It'sreally exciting that even after over 60 years of research in the area,there is more to learn about how blood interacts with materials,"Elbert said.


Story Source:

The above story is based on materials provided by Washington University in St. Louis. Note: Materials may be edited for content and length.


Cite This Page:

Washington University in St. Louis. "Researchers Find Multiple Proteins That Stick To Medical Devices." ScienceDaily. ScienceDaily, 6 October 2005. <www.sciencedaily.com/releases/2005/10/051006083856.htm>.
Washington University in St. Louis. (2005, October 6). Researchers Find Multiple Proteins That Stick To Medical Devices. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2005/10/051006083856.htm
Washington University in St. Louis. "Researchers Find Multiple Proteins That Stick To Medical Devices." ScienceDaily. www.sciencedaily.com/releases/2005/10/051006083856.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Matter & Energy News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

NASA's First 3-D Printer In Space Creates Its First Object

NASA's First 3-D Printer In Space Creates Its First Object

Newsy (Nov. 26, 2014) The International Space Station is now using a proof-of-concept 3D printer to test additive printing in a weightless, isolated environment. Video provided by Newsy
Powered by NewsLook.com
Bolivian Recycling Initiative Turns Plastic Waste Into School Furniture

Bolivian Recycling Initiative Turns Plastic Waste Into School Furniture

Reuters - Innovations Video Online (Nov. 26, 2014) Innovative recycling project in La Paz separates city waste and converts plastic garbage into school furniture made from 'plastiwood'. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Blu-Ray Discs Getting Second Run As Solar Panels

Blu-Ray Discs Getting Second Run As Solar Panels

Newsy (Nov. 26, 2014) Researchers at Northwestern University are repurposing Blu-ray movies for better solar panel technology thanks to the discs' internal structures. Video provided by Newsy
Powered by NewsLook.com
Today's Prostheses Are More Capable Than Ever

Today's Prostheses Are More Capable Than Ever

Newsy (Nov. 26, 2014) Advances in prosthetics are making replacement body parts stronger and more lifelike than they’ve ever been. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins