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First detailed view of morphing Parkinson's protein revealed

Date:
September 6, 2013
Source:
Purdue University
Summary:
Researchers have taken detailed images and measurements of the morphing structure of a brain protein thought to play a role in Parkinson's disease, information that could aid the development of medications to treat the condition.

This graphic illustrates the morphing structure of a brain protein thought to play a role in Parkinson's disease, information that could aid the development of medications to treat the condition. Researchers have taken detailed images and measurements of the changing structure for the first time. Findings reveal that the protein morphs from its globular shape into "protofibril" strands that assemble into pore-like rings. These rings then open up, forming pairs of protofibrils that assemble into fibrils through hydrogen bonds.
Credit: Purdue University/ Hangyu Zhang

Researchers have taken detailed images and measurements of the morphing structure of a brain protein thought to play a role in Parkinson's disease, information that could aid the development of medications to treat the condition.

The protein, called alpha synuclein (pronounced sine-yoo-cline), ordinarily exists in a globular shape. However, the protein morphs into harmful structures known as amyloid fibrils, which are linked to protein molecules that form in the brains of patients with neurodegenerative diseases.

"The abnormal protein formation characterizes a considerable number of human diseases, such as Alzheimer's, Parkinson's and Huntington's diseases and type II diabetes," said Lia Stanciu, an associate professor of materials engineering at Purdue University.

Until now, the transition from globular to fibrils had not been captured and measured.

Researchers incubated the protein in a laboratory and then used an electron microscope and a technique called cryoelectron microscopy to snap thousands of pictures over 24 hours, capturing its changing shape. The protein was frozen at specific time intervals with liquid nitrogen.

Findings reveal that the protein morphs from its globular shape into "protofibril" strands that assemble into pore-like rings. These rings then open up, forming pairs of protofibrils that assemble into fibrils through hydrogen bonds.

"We found a correlation between protofibrils in these rings and the fibrils, for the first time to our knowledge, by measuring their true sizes and visualizing the aggregation steps," Stanciu said. "A better understanding of the mechanism yields fresh insight into the pathogenesis of amyloid-related diseases and may provide us the opportunity to develop additional therapeutic strategies."

Parkinson's disease affects 1 percent to 2 percent of people older than 60, and an increase in its prevalence is anticipated in coming decades.

The findings were detailed in a research paper appearing in the June issue of the Biophysical Journal. The paper was authored by doctoral student Hangyu Zhang; former postdoctoral research associate Amy Griggs; Jean-Christophe Rochet, an associate professor of medicinal chemistry and molecular pharmacology; and Stanciu.

The researchers caused the protein to morph into fibrils by exposing it to copper, mimicking what happens when people are exposed to lead and other heavy metals. The contaminants interfere with the protein, changing the oxidation states of ions in its structure.

The research was funded by the National Institutes of Health. Future work will include experiments focusing on what happens when higher concentrations of copper are used.


Story Source:

The above story is based on materials provided by Purdue University. The original article was written by Emil Venere. Note: Materials may be edited for content and length.


Journal Reference:

  1. Hangyu Zhang, Amy Griggs, Jean-Christophe Rochet, and Lia A. Stanciu. In Vitro Study of a-Synuclein Protofibrils by Cryo-EM Suggests a Cu2D-Dependent Aggregation Pathway. Biophysical Journal, 2013 (in press)

Cite This Page:

Purdue University. "First detailed view of morphing Parkinson's protein revealed." ScienceDaily. ScienceDaily, 6 September 2013. <www.sciencedaily.com/releases/2013/09/130906102532.htm>.
Purdue University. (2013, September 6). First detailed view of morphing Parkinson's protein revealed. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2013/09/130906102532.htm
Purdue University. "First detailed view of morphing Parkinson's protein revealed." ScienceDaily. www.sciencedaily.com/releases/2013/09/130906102532.htm (accessed October 20, 2014).

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