Featured Research

from universities, journals, and other organizations

Novel DNA Vaccine Leads To Kidney Damage Prevention In Systemic Lupus Erythematosus Models

Date:
June 18, 2009
Source:
European League Against Rheumatism
Summary:
DNA vaccination using lupus autoantigens and interleukin-10 (IL-10, a cytokine that plays an important role in regulating the immune system) has potential as a novel therapy to induce antigen specific tolerance and may help to prevent kidney damage in patients with systemic lupus erythematosus, according to a new study.

DNA vaccination using lupus autoantigens and interleukin-10 (IL-10, a cytokine that plays an important role in regulating the immune system) has potential as a novel therapy to induce antigen specific tolerance and may help to prevent kidney damage in patients with systemic lupus erythematosus (SLE), according to a new study presented June 11 at EULAR 2009, the Annual Congress of the European League Against Rheumatism in Copenhagen, Denmark.

Related Articles


SLE is a complex, chronic autoimmune disease that causes inflammation and damage to body tissues. Inflammation of the kidney can cause kidney failure, often a significant complication of SLE associated with the more severe forms of the disease, and the primary cause of disability that leads to other fatal complications in people with the condition.

In the study, one group of experimental mice was vaccinated with Sm (Smith antigens that are small nuclear ribonucleoproteins: snRNPs) lupus autoantigen in combination with IL-10 and another group vaccinated with the same antigen and IFN-gamma, both signaling molecules involved in the immune process. SLE was induced in the mice and the levels of antibodies against various lupus autoantibodies were analysed. The study found that levels of anti-Sm antibodies in the IL-10 vaccinated group were lower than those in the group vaccinated with IFN-gamma (p=0.026, statistically significant).

Interestingly, the prevalence and levels of other autoantibodies such as anti-Argonaute2 (Ago2)/Su and chromatin did not differ between the groups, indicating that the vaccination was Sm antigen-specific and successfully targeting particular antibodies. Significant proteinuria (a urinary condition which can indicate kidney damage) (>1.5+) was less common in the IL-10 vaccinated group compared with the IFN-gamma group, which may suggest a beneficial role for IL-10 vaccination in preventing kidney damage associated with SLE.

Dr Monica Vazquez del Mercado, Head of the Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético of the Universidad de Guadalajara, Mexico, corresponding author of the study, said: "Some autoantibodies are specific for SLE and are considered to be characteristic of the condition. The mechanisms that regulate the production of these antibodies are poorly understood, however, the results of our study have identified one possible way of influencing the pathway behind kidney damage in SLE."

Studies in human and mouse models of lupus have revealed that the major epitopes of the anti-Sm antibodies are present at specific locations (D1, D2 and B/B´) of the Sm antigens. Researchers in this study prepared DNA vaccines of Sm D1, D2, B/B´, B/B´COOH, IFN-gamma and IL-10 using direct cloning techniques with pcDNA"3.1D/V5-His-TOPO® and purified these using Qiagen Endo-Free Giga Prep Kit. Eight groups of 6 week-old female BALB/c mice (13/group) received intramuscular injections of 100 micrograms of the vaccination preparation at days 2 and 9. At day 16, mice were induced with experimental SLE.

Serum samples were collected at day 0 and then monthly for analysis of autoantibodies and immunoglobulin levels. Serum autoantibodies were tested by immunoprecipitation and ELISA. Proteinuria (excessive protein in the urine) was assessed monthly using Multistix®. Kidney pathology and immune complex deposition were examined at 6 months.


Story Source:

The above story is based on materials provided by European League Against Rheumatism. Note: Materials may be edited for content and length.


Cite This Page:

European League Against Rheumatism. "Novel DNA Vaccine Leads To Kidney Damage Prevention In Systemic Lupus Erythematosus Models." ScienceDaily. ScienceDaily, 18 June 2009. <www.sciencedaily.com/releases/2009/06/090611110952.htm>.
European League Against Rheumatism. (2009, June 18). Novel DNA Vaccine Leads To Kidney Damage Prevention In Systemic Lupus Erythematosus Models. ScienceDaily. Retrieved November 29, 2014 from www.sciencedaily.com/releases/2009/06/090611110952.htm
European League Against Rheumatism. "Novel DNA Vaccine Leads To Kidney Damage Prevention In Systemic Lupus Erythematosus Models." ScienceDaily. www.sciencedaily.com/releases/2009/06/090611110952.htm (accessed November 29, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, November 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Rural India's Low-Cost Sanitary Pad Revolution

Rural India's Low-Cost Sanitary Pad Revolution

AFP (Nov. 28, 2014) — One man hopes his invention -– a machine that produces cheap sanitary pads –- will help empower Indian women. Duration: 01:51 Video provided by AFP
Powered by NewsLook.com
Research on Bats Could Help Develop Drugs Against Ebola

Research on Bats Could Help Develop Drugs Against Ebola

AFP (Nov. 28, 2014) — In Africa's only biosafety level 4 laboratory, scientists have been carrying out experiments on bats to understand how virus like Ebola are being transmitted, and how some of them resist to it. Duration: 01:18 Video provided by AFP
Powered by NewsLook.com
WHO Says Male Ebola Survivors Should Abstain From Sex

WHO Says Male Ebola Survivors Should Abstain From Sex

Newsy (Nov. 28, 2014) — WHO cites four studies that say Ebola can still be detected in semen up to 82 days after the onset of symptoms. Video provided by Newsy
Powered by NewsLook.com
Ebola Leaves Orphans Alone in Sierra Leone

Ebola Leaves Orphans Alone in Sierra Leone

AFP (Nov. 27, 2014) — The Ebola epidemic sweeping Sierra Leone is having a profound effect on the country's children, many of whom have been left without any family members to support them. Duration: 01:02 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins