Featured Research

from universities, journals, and other organizations

Genetic abnormalities identified in pluripotent stem cell lines

Date:
January 6, 2011
Source:
University of California - San Diego
Summary:
A multinational team of researchers has documented specific genetic abnormalities that occur in human embryonic and induced pluripotent stem cell lines. The published findings highlight the need for frequent genomic monitoring of pluripotent stem cells to assure their stability and clinical safety.

A multinational team of researchers led by stem cell scientists at the University of California, San Diego School of Medicine and Scripps Research Institute has documented specific genetic abnormalities that occur in human embryonic (hESC) and induced pluripotent stem cell (iPSC) lines.

Related Articles


Their study will be published in the January 7 issue of the journal Cell Stem Cell.

The published findings highlight the need for frequent genomic monitoring of pluripotent stem cells to assure their stability and clinical safety.

"We found that human pluripotent cells (hESCs and iPSCs) had higher frequencies of genomic aberrations than other cell types," said Louise Laurent, MD, PhD, assistant professor in the UCSD Department of Reproductive Medicine and first author on the study. "Most strikingly, we observed a higher frequency of genomic duplications in hESCs and deletions in iPSCs, when compared to non-pluripotent samples."

The ability of human pluripotent stem cells to become every cell type in the body has made them potential sources of differentiated cells for cell replacement therapies. "Since genetic aberrations are often associated with cancers, it is vital that cell lines destined for clinical use are free from cancer-associated genomic alterations," said senior author Jeanne F. Loring, PhD, professor and Director of the Center for Regenerative Medicine at the Scripps Research Institute.

The team identified regions in the genome that had a greater tendency to become abnormal in pluripotent cell lines. With hESCs, the observed abnormalities were most often duplications near pluripotency-associated genes; in iPSC lines, there were duplications involving cell proliferation genes and deletions associated with tumor suppressor genes.

These changes could not have been detected by traditional microscopic techniques such as karyotyping. The team instead used a high-resolution molecular technique called "single nucleotide polymorphism" (SNP) analysis, which allowed them to look for genetic changes at more than a million sites in the human genome.

"We were surprised to see profound genetic changes occurring in some cultures over very short periods of time, such as during the process of reprogramming somatic cells into iPSCs and during differentiation of the cells in culture," Laurent said. "We don't know yet what effects, if any, these genetic abnormalities will have on the outcome of basic research studies or clinical applications, and we need to find out."

Loring concluded: "The results of the study illustrate the need for frequent genomic monitoring of pluripotent stem cell cultures. SNP analysis has not been a part of routine monitoring of hESC and iPSC cultures, but our results suggest that perhaps it should be."

Additional contributors to the paper include Ileana Slavin, Ha Tran, Candace Lynch, Sherman Ku, and Joel Gottesfeld, The Scripps Research Institute; Robert Morey, UC San Diego and The Scripps Research Institute; Franz-Josef Muller, Zentrum für Integrative Psychiatrie, Kiel, Germany and The Scripps Research Institute; Andrew Schork and Carolline M. Nievergelt, UC San Diego; Julie V. Harness and Hans S. Keirstead, UC Irvine; Sunray Lee and Hyun-Sook Park, Modern Cell & Tissue Technologies Inc., Seoul, South Korea; Maria J. Barrero and Juan Carlos Izpisua Belmonte, Salk Institute for Biological Studies and Centro de Medicina Regenerativa de Barcelona; Marina Martynova and Rusian Semechkin, International Stem Cell Corporation, Oceanside, CA; Vasiliy Galat, Northwestern University; Chuck Murry, University of Washington; Ulrich Schmidt, Sydney IVF Stem Cell Laboratory, Sydney, Australia; Andrew Laslett, Commonwealth Scientific and Industrial Research Organisation, Clayton, Australia and Monash University, Victoria, Australia; and Ron Shamir, Tel Aviv University.

The study was funded by the National Institutes of Health, the California Institute for Regenerative Medicine, the Hartwell Foundation, the Millipore Foundation, the Esther O'Keefe Foundation, the Edmond J. Safra foundation in Tel Aviv, the Legacy stem cell research fund, the PEW Charitable Trust, the South Korea Ministry of Education, Science and Technology, the Ministerio de Ciencia e Innovación of Spain, MICINN Fundacion Cellex, the G. Harold and Leila Y. Mathers Charitable Foundation and Sanofi-Aventis.


Story Source:

The above story is based on materials provided by University of California - San Diego. The original article was written by Debra Kain. Note: Materials may be edited for content and length.


Journal Reference:

  1. Louise C. Laurent, Igor Ulitsky, Ileana Slavin, Ha Tran, Andrew Schork, Robert Morey, Candace Lynch, Julie V. Harness, Sunray Lee, Maria J. Barrero, Sherman Ku, Marina Martynova, Ruslan Semechkin, Vasiliy Galat, Joel Gottesfeld, Juan Carlos Izpisua Belmonte, Chuck Murry, Hans S. Keirstead, Hyun-Sook Park, Uli Schmidt, Andrew L. Laslett, Franz-Josef Muller, Caroline M. Nievergelt, Ron Shamir, Jeanne F. Loring. Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ESCs and iPSCs during Reprogramming and Time in Culture. Cell Stem Cell, 2011; 8 (1): 106-118 DOI: 10.1016/j.stem.2010.12.003

Cite This Page:

University of California - San Diego. "Genetic abnormalities identified in pluripotent stem cell lines." ScienceDaily. ScienceDaily, 6 January 2011. <www.sciencedaily.com/releases/2011/01/110106144737.htm>.
University of California - San Diego. (2011, January 6). Genetic abnormalities identified in pluripotent stem cell lines. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2011/01/110106144737.htm
University of California - San Diego. "Genetic abnormalities identified in pluripotent stem cell lines." ScienceDaily. www.sciencedaily.com/releases/2011/01/110106144737.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Leaves Orphans Alone in Sierra Leone

Ebola Leaves Orphans Alone in Sierra Leone

AFP (Nov. 27, 2014) — The Ebola epidemic sweeping Sierra Leone is having a profound effect on the country's children, many of whom have been left without any family members to support them. Duration: 01:02 Video provided by AFP
Powered by NewsLook.com
Experimental Ebola Vaccine Shows Promise In Human Trial

Experimental Ebola Vaccine Shows Promise In Human Trial

Newsy (Nov. 27, 2014) — A recent test of a prototype Ebola vaccine generated an immune response to the disease in subjects. Video provided by Newsy
Powered by NewsLook.com
Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) — Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
Today's Prostheses Are More Capable Than Ever

Today's Prostheses Are More Capable Than Ever

Newsy (Nov. 26, 2014) — Advances in prosthetics are making replacement body parts stronger and more lifelike than they’ve ever been. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins