Featured Research

from universities, journals, and other organizations

Scientists decode 'software' instructions of aggressive leukemia cells

Date:
October 29, 2012
Source:
NewYork-Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College
Summary:
Scientists have decoded the key "software" instructions that drive three of the most virulent forms of acute lymphoblastic leukemia (ALL). They discovered ALL's "software" is encoded with epigenetic marks, chemical modifications of DNA and surrounding proteins, allowing the research team to identify new potential biomarkers and therapeutic targets.

A team of national and international researchers, led by Weill Cornell Medical College scientists, have decoded the key "software" instructions that drive three of the most virulent forms of acute lymphoblastic leukemia (ALL). They discovered ALL's "software" is encoded with epigenetic marks, chemical modifications of DNA and surrounding proteins, allowing the research team to identify new potential biomarkers and therapeutic targets.

The research, published in Cancer Discovery, is the first study to show how these three different forms of white blood cell cancer are epigenetically programmed by several different molecules controlling cascading biological networks that manipulate normal gene function, directing cancer development and growth.

"Epigenetic programming is the software that is written on to human DNA, which can be viewed as its hard drive. This programming contains the instructions that determine how cells including leukemia cells function and cause disease," says the study's lead investigator, Dr. Ari Melnick, associate professor of medicine and director of the Raymond and Beverly Sackler Center for Biomedical and Physical Sciences at Weill Cornell Medical College.

"Finding the instructions that ultimately lead to cancer development, and to the especially bad outcome seen in patients with these different forms of ALL, is especially urgent. Epigenetic instructions are contained in many chemical layers. Our study is the first to integrate the decoding of many layers simultaneously, which has enabled us to unlock some of the mysteries explaining the malignant and aggressive behavior of these leukemias," says Dr. Melnick, who is also a hematologist-oncologist at NewYork-Presbyterian Hospital/Weill Cornell Medical Center.

Abnormal Epigenetic Programming Leads to Poor Outcomes

The research team examined abnormalities in the "software" epigenetic programming that leads to the three forms of adult B-acute lymphoblastic leukemia (B-ALL), the most common form of ALL. These forms are BCR-ABL1-positive B-ALL, E2A-PBX1-positive B-ALL, and MLLr-B-ALL. These three B-ALL subtypes feature mutations of different master regulatory genes which force bone marrow cells to produce cancer-promoting proteins. Long-term survival is less than 40 percent among these patients.

"Similar to normal tissue, we believe that tumors may be dependent on specific patterns of epigenetic programming -- especially in B-ALL, where studies suggests epigenomic programming is globally disrupted," Dr. Melnick says. "Our goal was to identify epigenetically modified genes and the molecular machines that cause them to become abnormally programmed."

To that end, the research team performed DNA methylation and gene expression profiling on 215 adult B-ALL patients enrolled in the ECOG E2993 clinical trial, a multi-center and multi-national study, testing different forms of treatment in patients with ALL.

Researchers identified core epigenetic gene signatures that were associated with abnormal fusion proteins. In the case of BCR-ABL1-positive B-ALL, they found that the most deregulated gene network centered around an extraordinarily epigenetically deregulated gene they identified as interleukin-2 receptor alpha, which encodes a protein called CD25.

"Among patients who had BCR-ABL1-positive B-ALL, it was those with aberrant epigenetic programming of CD25 that had significantly worse outcome," says Dr. Melnick. "It's the patients that have this programming glitch that do really poorly."

Although the researchers don't yet know what CD25 does, and why it is important, they say CD25 will be a useful biomarker to test for patients that are at highest risk for poorer outcome.

Dr. Melnick stresses that therapeutic antibodies to the CD25 protein already exist that can, theoretically, destroy leukemic cells expressing this protein. The research team showed that using the CD25 antibody successfully killed BCR-ABL1-positive B-ALL in laboratory experiments. "One could potentially conceive of a human clinical trial where those antibodies are used to attack these cancerous cells," he says.

Researchers also discovered what is writing the bad software in the other two B-ALL subtypes. In both cases, the abnormal cancer proteins E2A-PBX1 and MLLr turn out to be directly involved in altering the epigenetic programming of leukemic cells. Remarkably, MLLr epigenetically turns on a powerful cancer protein called BCL6. In the study, drugs developed by Dr. Melnick that block BCL6 activity potently killed and suppressed the ALL cells in patients enrolled in this clinical trial, which warrants the testing of BCL6 inhibitors in this aggressive form of ALL.

"This study links the direct actions of oncogenic fusion proteins with disruption of epigenetic regulation that leads to abnormal production of cancer-driving genes," Dr. Melnick says. "It potentially provides us with a biomarker for cancer outcomes as well as potential treatments in these aggressive forms of leukemia."

This research study was supported by the Chemotherapy Foundation, Burroughs Wellcome Foundation, The Leukemia & Lymphoma Society and the Sackler Center for Biomedical and Physical Sciences at the Weill Cornell Medical College.

Study co-authors include Dr. Sarah Brennan, Yushan Li, Dr. Chuanxin Huang, Yuan Xin, Dr. Monica L. Guzman and Dr. Olivier Elemento from Weill Cornell; Dr. Huimin Geng, formerly of Weill Cornell and now at University of California, San Francisco; Dr. Janis Racevskis and Dr. Elisabeth Paietta from Albert Einstein College of Medicine; Dr. Thomas A. Milne, Dr. Wei-Yi Chen, Dr. Debabrata Biswa, Dr. C. David Allis and Dr. Robert G. Roeder from Rockefeller University; Christian Hurtz, Dr. Soo-Mi Kweon, Dr. Seyedmehdi Shojaee and Dr. Markus Mόschen from the University of Southern California, Los Angeles; Lynette Zickl and Dr. Donna Neuberg from the Dana Farber Cancer Institute, Boston; Dr. Rhett P. Ketterling and Dr. Mark R. Litzow from the Mayo Clinic, Rochester, Minnesota; Dr. Selina M. Luger from the University of Pennsylvania; Dr. Martin S. Tallman from the Memorial Sloan-Kettering Cancer Center; Dr. Jacob M. Rowe from the Rambam Medical Center in Haifa, Israel; and Dr. Hillard Lazarus from Case Western Reserve University in Cleveland, Ohio.


Story Source:

The above story is based on materials provided by NewYork-Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College. Note: Materials may be edited for content and length.


Cite This Page:

NewYork-Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College. "Scientists decode 'software' instructions of aggressive leukemia cells." ScienceDaily. ScienceDaily, 29 October 2012. <www.sciencedaily.com/releases/2012/10/121029103504.htm>.
NewYork-Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College. (2012, October 29). Scientists decode 'software' instructions of aggressive leukemia cells. ScienceDaily. Retrieved September 1, 2014 from www.sciencedaily.com/releases/2012/10/121029103504.htm
NewYork-Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College. "Scientists decode 'software' instructions of aggressive leukemia cells." ScienceDaily. www.sciencedaily.com/releases/2012/10/121029103504.htm (accessed September 1, 2014).

Share This




More Health & Medicine News

Monday, September 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

We've Got Mites Living In Our Faces And So Do You

We've Got Mites Living In Our Faces And So Do You

Newsy (Aug. 30, 2014) — A new study suggests 100 percent of adult humans (those over 18 years of age) have Demodex mites living in their faces. Video provided by Newsy
Powered by NewsLook.com
Liberia Continues Fight Against Ebola

Liberia Continues Fight Against Ebola

AFP (Aug. 30, 2014) — Authorities in Liberia try to stem the spread of the Ebola epidemic by raising awareness and setting up sanitation units for people to wash their hands. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com
California Passes 'yes-Means-Yes' Campus Sexual Assault Bill

California Passes 'yes-Means-Yes' Campus Sexual Assault Bill

Reuters - US Online Video (Aug. 30, 2014) — California lawmakers pass a bill requiring universities to adopt "affirmative consent" language in their definitions of consensual sex, part of a nationwide drive to curb sexual assault on campuses. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
New Drug Could Reduce Cardiovascular Deaths

New Drug Could Reduce Cardiovascular Deaths

Newsy (Aug. 30, 2014) — The new drug from Novartis could reduce cardiovascular deaths by 20 percent compared to other similar drugs. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins