Featured Research

from universities, journals, and other organizations

Better Peroxidase Improves Disease Diagnosis

Date:
July 15, 1997
Source:
Purdue University
Summary:
When Purdue University geneticist Rick Vierling first looked for ways to add value to soybeans, he didn't expect that he could help doctors diagnose AIDS in China. But that's exactly where his research is leading.

Purdue University News Service 1132 Engineering Administration Building West Lafayette, IN 47907-1132 Voice: 765-494-2096 FAX: 765-494-0401

July 11, 1997

BETTER PEROXIDASE IMPROVES DISEASE DIAGNOSIS

WEST LAFAYETTE, Ind. -- When Purdue University geneticist Rick Vierling first looked for ways to add value to soybeans, he didn't expect that he could help doctors diagnose AIDS in China. But that's exactly where his research is leading.

In June, the Indiana Crop Improvement Association (ICIA) licensed the use of soybean peroxidase in medical diagnostic test kits to Enzymol International Inc., Columbus, Ohio, and to American Qualex, San Clemente, Calif.

In July, American Qualex will announce the use of soybean peroxidase-based compounds in new diagnostic kits at the annual meeting of the American Association of Clinical Chemistry in Atlanta, July 22-23. The company also plans to collaborate with diagnostic, biotechnology, life science and pharmaceutical companies to develop other products that use soybean peroxidase.

The soy enzyme replaces horseradish peroxidase, which is an integral part of kits designed to help diagnose a myriad of viral, bacterial and parasitic diseases, including AIDS and malaria. Standard kits lose effectiveness in about four months without refrigeration, according to industry sources. Vierling's preliminary research suggests that kits made with soybean peroxidase should last unrefrigerated for at least a year. Such kits will be very useful in places such as China, Africa and Central America, he says.

Vierling, director of the Genetics Laboratory for the ICIA, started experimenting with soybean peroxidase because it is a hot "green" industrial commodity that can be extracted from soybean seed coats. Vierling says researchers have found ways to extract peroxidase from the soybean hulls without reducing the value of the oil or the meal in the beans.

"Peroxidase replaces a lot of really harsh chemicals," says Alex Pokora, vice president for technology for Enzymol, a worldwide leader in developing peroxidase technology. "It's a cleaner technology, and it's cost effective."

Manufacturers became interested in soybean peroxidase because the supply of horseradish peroxidase was limited and the horseradish enzyme wasn't very stable at high temperatures. Researchers from Enzymol asked Vierling to test soybean peroxidase and to look for soybean varieties with a higher peroxidase content.

Vierling immediately confirmed that the soy enzyme worked better.

"All plants contain peroxidase, but not all peroxidase is created equal," Vierling says. "Soybean peroxidase is highly reactive and thermally stable. I don't think there's a better peroxidase out there."

As Vierling measured enzyme levels in beans, he saw that a quick, chemical test for peroxidase activity would greatly speed his research and plant breeding efforts. He developed one -- and in doing so, he noted that his technique was similar to medical diagnostic test kits that use horseradish peroxidase. He suspected that the medical kits might work better if they were made with the soy peroxidase. Preliminary tests confirmed his suspicions.

For further confirmation, Vierling sent samples of soybean peroxidase to John Morrow, a professor at Texas Tech University's Health Science Center in Lubbock, Texas. Morrow and Vierling had worked together when Vierling was a graduate student. Vierling asked Morrow to substitute soybean peroxidase for horseradish peroxidase in clinical diagnostic tests.

"I was lukewarm about the idea at first," says Morrow, "but when I tried it, I was converted. I think he's got something that's a nice improvement over current technology."

In addition to its superior performance in medical and industrial applications, soybean peroxidase is relatively easy to obtain. The beans are widely grown, and production, transportation and storage facilities already are in place to deliver high-peroxidase beans for enzyme extraction.

ACS Code: 9707 Ag Vierling.license/9706a15

Sources: Rick Vierling, (765) 474-3494; e-mail, vierling@mail.dcwi.com

Alex Pokora, (614) 856-3050

John Morrow, (806)743-2509

Writer: Rebecca Goetz (765) 494-0461; e-mail, rjg@aes.purdue.edu


Story Source:

The above story is based on materials provided by Purdue University. Note: Materials may be edited for content and length.


Cite This Page:

Purdue University. "Better Peroxidase Improves Disease Diagnosis." ScienceDaily. ScienceDaily, 15 July 1997. <www.sciencedaily.com/releases/1997/07/970715053558.htm>.
Purdue University. (1997, July 15). Better Peroxidase Improves Disease Diagnosis. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/1997/07/970715053558.htm
Purdue University. "Better Peroxidase Improves Disease Diagnosis." ScienceDaily. www.sciencedaily.com/releases/1997/07/970715053558.htm (accessed October 21, 2014).

Share This



More Health & Medicine News

Tuesday, October 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

How Nigeria Beat Its Ebola Outbreak

How Nigeria Beat Its Ebola Outbreak

Newsy (Oct. 20, 2014) The World Health Organization has declared Nigeria free of Ebola. Health experts credit a bit of luck and the government's initial response. Video provided by Newsy
Powered by NewsLook.com
Another Study Suggests Viagra Is Good For The Heart

Another Study Suggests Viagra Is Good For The Heart

Newsy (Oct. 20, 2014) An ingredient in erectile-dysfunction medications such as Viagra could improve heart function. Perhaps not surprising, given Viagra's history. Video provided by Newsy
Powered by NewsLook.com
Ebola Worries End for Dozens on U.S. Watch Lists

Ebola Worries End for Dozens on U.S. Watch Lists

Reuters - US Online Video (Oct. 20, 2014) Forty-three people who had contact with Thomas Eric Duncan, the first person diagnosed with Ebola in the U.S., were cleared overnight of twice-daily monitoring after 21 days of showing no symptoms. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
CDC Calls for New Ebola Safety Guidelines

CDC Calls for New Ebola Safety Guidelines

AP (Oct. 20, 2014) Centers for Disease Control and Prevention Director Dr. Tom Frieden laid out new guidelines for health care workers when dealing with the deadly Ebola virus including new precautions when taking off personal protective equipment. (Oct. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins