Featured Research

from universities, journals, and other organizations

Better Peroxidase Improves Disease Diagnosis

Date:
July 15, 1997
Source:
Purdue University
Summary:
When Purdue University geneticist Rick Vierling first looked for ways to add value to soybeans, he didn't expect that he could help doctors diagnose AIDS in China. But that's exactly where his research is leading.

Purdue University News Service 1132 Engineering Administration Building West Lafayette, IN 47907-1132 Voice: 765-494-2096 FAX: 765-494-0401

Related Articles


July 11, 1997

BETTER PEROXIDASE IMPROVES DISEASE DIAGNOSIS

WEST LAFAYETTE, Ind. -- When Purdue University geneticist Rick Vierling first looked for ways to add value to soybeans, he didn't expect that he could help doctors diagnose AIDS in China. But that's exactly where his research is leading.

In June, the Indiana Crop Improvement Association (ICIA) licensed the use of soybean peroxidase in medical diagnostic test kits to Enzymol International Inc., Columbus, Ohio, and to American Qualex, San Clemente, Calif.

In July, American Qualex will announce the use of soybean peroxidase-based compounds in new diagnostic kits at the annual meeting of the American Association of Clinical Chemistry in Atlanta, July 22-23. The company also plans to collaborate with diagnostic, biotechnology, life science and pharmaceutical companies to develop other products that use soybean peroxidase.

The soy enzyme replaces horseradish peroxidase, which is an integral part of kits designed to help diagnose a myriad of viral, bacterial and parasitic diseases, including AIDS and malaria. Standard kits lose effectiveness in about four months without refrigeration, according to industry sources. Vierling's preliminary research suggests that kits made with soybean peroxidase should last unrefrigerated for at least a year. Such kits will be very useful in places such as China, Africa and Central America, he says.

Vierling, director of the Genetics Laboratory for the ICIA, started experimenting with soybean peroxidase because it is a hot "green" industrial commodity that can be extracted from soybean seed coats. Vierling says researchers have found ways to extract peroxidase from the soybean hulls without reducing the value of the oil or the meal in the beans.

"Peroxidase replaces a lot of really harsh chemicals," says Alex Pokora, vice president for technology for Enzymol, a worldwide leader in developing peroxidase technology. "It's a cleaner technology, and it's cost effective."

Manufacturers became interested in soybean peroxidase because the supply of horseradish peroxidase was limited and the horseradish enzyme wasn't very stable at high temperatures. Researchers from Enzymol asked Vierling to test soybean peroxidase and to look for soybean varieties with a higher peroxidase content.

Vierling immediately confirmed that the soy enzyme worked better.

"All plants contain peroxidase, but not all peroxidase is created equal," Vierling says. "Soybean peroxidase is highly reactive and thermally stable. I don't think there's a better peroxidase out there."

As Vierling measured enzyme levels in beans, he saw that a quick, chemical test for peroxidase activity would greatly speed his research and plant breeding efforts. He developed one -- and in doing so, he noted that his technique was similar to medical diagnostic test kits that use horseradish peroxidase. He suspected that the medical kits might work better if they were made with the soy peroxidase. Preliminary tests confirmed his suspicions.

For further confirmation, Vierling sent samples of soybean peroxidase to John Morrow, a professor at Texas Tech University's Health Science Center in Lubbock, Texas. Morrow and Vierling had worked together when Vierling was a graduate student. Vierling asked Morrow to substitute soybean peroxidase for horseradish peroxidase in clinical diagnostic tests.

"I was lukewarm about the idea at first," says Morrow, "but when I tried it, I was converted. I think he's got something that's a nice improvement over current technology."

In addition to its superior performance in medical and industrial applications, soybean peroxidase is relatively easy to obtain. The beans are widely grown, and production, transportation and storage facilities already are in place to deliver high-peroxidase beans for enzyme extraction.

ACS Code: 9707 Ag Vierling.license/9706a15

Sources: Rick Vierling, (765) 474-3494; e-mail, [email protected]

Alex Pokora, (614) 856-3050

John Morrow, (806)743-2509

Writer: Rebecca Goetz (765) 494-0461; e-mail, [email protected]


Story Source:

The above story is based on materials provided by Purdue University. Note: Materials may be edited for content and length.


Cite This Page:

Purdue University. "Better Peroxidase Improves Disease Diagnosis." ScienceDaily. ScienceDaily, 15 July 1997. <www.sciencedaily.com/releases/1997/07/970715053558.htm>.
Purdue University. (1997, July 15). Better Peroxidase Improves Disease Diagnosis. ScienceDaily. Retrieved December 22, 2014 from www.sciencedaily.com/releases/1997/07/970715053558.htm
Purdue University. "Better Peroxidase Improves Disease Diagnosis." ScienceDaily. www.sciencedaily.com/releases/1997/07/970715053558.htm (accessed December 22, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Monday, December 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Christmas Kissing Good for Health

Christmas Kissing Good for Health

Reuters - Innovations Video Online (Dec. 22, 2014) Scientists in Amsterdam say couples transfer tens of millions of microbes when they kiss, encouraging healthy exposure to bacteria. Suzannah Butcher reports. Video provided by Reuters
Powered by NewsLook.com
Brain-Dwelling Tapeworm Reveals Genetic Secrets

Brain-Dwelling Tapeworm Reveals Genetic Secrets

Reuters - Innovations Video Online (Dec. 22, 2014) Cambridge scientists have unravelled the genetic code of a rare tapeworm that lived inside a patient's brain for at least four year. Researchers hope it will present new opportunities to diagnose and treat this invasive parasite. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Americans Drink More in the Winter

Americans Drink More in the Winter

Buzz60 (Dec. 22, 2014) The BACtrack breathalyzer app analyzed Americans' blood alcohol content and found out a whole lot of interesting things about their drinking habits. Mara Montalbano (@maramontalbano) has more. Video provided by Buzz60
Powered by NewsLook.com
Touch-Free Smart Phone Empowers Mobility-Impaired

Touch-Free Smart Phone Empowers Mobility-Impaired

Reuters - Innovations Video Online (Dec. 21, 2014) A touch-free phone developed in Israel enables the mobility-impaired to operate smart phones with just a movement of the head. Suzannah Butcher reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins