Featured Research

from universities, journals, and other organizations

New Way To Modify Mammalian Genes: Honolulu Transgenesis

Date:
May 17, 1999
Source:
University Of Hawaii
Summary:
University of Hawaii scientists, together with colleagues in Japan, have developed a new method for producing transgenic mammals. The method - Honolulu transgenesis - uses sperm to deliver genetic information (DNA) from one organism into the egg of another. The egg can then divide so that every cell in the new individual contains the introduced DNA.

University of Hawaii scientists, together with colleagues in Japan, have developed a new method for producing transgenic mammals. The method - Honolulu transgenesis - uses sperm to deliver genetic information (DNA) from one organism into the egg of another. The egg can then divide so that every cell in the new individual contains the introduced DNA. The experiments demonstrated the new method using DNA for jellyfish green fluorescent protein to make green mice, and are reported in the May 14 issue of the journal, Science.

The team, whose members made headlines last summer with Honolulu cloning, reported success in producing transgenic mice by injecting eggs in a method called intracytoplasmic sperm injection. About one in five offspring resulting from the new procedure contain the introduced DNA.

The production of transgenic animals isn't new; transgenic mice were first produced in 1974, and the prevailing method of producing them by injection of DNA into the pronucleus of a one-cell embryo was first described in 1980. The pronucleus microinjection method is the most commonly used. It works well in mice, but is difficult in mammals such as cows and pigs, in which the pronucleus is difficult to locate within the cell, says Tony Perry, an assistant professor in the UH Manoa John A. Burns School of Medicine and corresponding author on the Science paper. "The great thing about this new method is that it is very straight forward," Perry says. "It may prove to be more efficient."

In Honolulu transgenesis, mouse sperm are fozen, freeze-dried, or treated with a chemical to disrupt their coat. The sperm are mixed with DNA (the researchers used from a jellyfish, but it could, in principle, be any DNA). Sperm and the DNA are then injected into oocytes. Developing embryos are transferred into a foster mother. The introduced DNA contains a gene that directs production of a protein that glows green under long-wave ultraviolet light, so the scientists could easily see that the jellyfish 'green gene' DNA was incorporated into the mouse genome if the mouse appeared green when illuminated by ultraviolet light.

The 'green gene' is just a marker to indicate that the technique works, says Perry. "We hope and believe that the technique will be useful in medical research and in development of xenotransplant donors," he says. Possible applications could include using the mouse as a model for studying the function of human genes. Also, more organs could be available for human transplant if pig genomes could be modified so that the corresponding pig organs wouldn't trigger a crtical immune rejection in patients who receive them.

Collaborators on the project include Teruhiko Wakayama, Ryuzo Yanagimachi, Hidefumi Kishikawa and Tsuyoshi Kasai of the University of Hawaii; Masaru Okabe of the Genome Information Research Center, Osaka University; and Yutaka Toyoda of the Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine.


Story Source:

The above story is based on materials provided by University Of Hawaii. Note: Materials may be edited for content and length.


Cite This Page:

University Of Hawaii. "New Way To Modify Mammalian Genes: Honolulu Transgenesis." ScienceDaily. ScienceDaily, 17 May 1999. <www.sciencedaily.com/releases/1999/05/990517064920.htm>.
University Of Hawaii. (1999, May 17). New Way To Modify Mammalian Genes: Honolulu Transgenesis. ScienceDaily. Retrieved September 22, 2014 from www.sciencedaily.com/releases/1999/05/990517064920.htm
University Of Hawaii. "New Way To Modify Mammalian Genes: Honolulu Transgenesis." ScienceDaily. www.sciencedaily.com/releases/1999/05/990517064920.htm (accessed September 22, 2014).

Share This



More Health & Medicine News

Monday, September 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Liberia Pleads for Help to Fight Ebola

Liberia Pleads for Help to Fight Ebola

AP (Sep. 22, 2014) Liberia's finance minister is urging the international community to quickly follow through on pledges of cash to battle Ebola. Bodies are piling up in the capital Monrovia as the nation awaits more help. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Ebola Doctor Says Border Controls Critical

Ebola Doctor Says Border Controls Critical

AP (Sep. 22, 2014) A Florida doctor who helped fight the expanding Ebola outbreak in West Africa says the disease can be stopped, but only if nations quickly step up their response and make border control a priority. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Global Ebola Aid Increasing But Critics Say It's Late

Global Ebola Aid Increasing But Critics Say It's Late

Newsy (Sep. 21, 2014) More than 100 tons of medical supplies were sent to West Africa on Saturday, but aid workers say the global response is still sluggish. Video provided by Newsy
Powered by NewsLook.com
Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins