Featured Research

from universities, journals, and other organizations

Robots Eavesdrop On Cellular Discussions

Date:
February 10, 2000
Source:
NIH-National Institute Of General Medical Sciences
Summary:
Using robots to monitor the goings-on of thousands of individual baker's yeast cells growing on a small plastic grid, scientists supported by the National Institutes of Health, in collaboration with CuraGen Corporation of New Haven, Connecticut, have accomplished a biological milestone in determining which molecules in a cell "talk" to others by making physical contact.

For the first time, scientists have figured out a way to record the "conversations" taking place simultaneously between thousands of molecules inside a single cell.

Using robots to monitor the goings-on of thousands of individual baker's yeast cells growing on a small plastic grid, scientists supported by the National Institutes of Health, in collaboration with CuraGen Corporation of New Haven, Connecticut, have accomplished a biological milestone in determining which molecules in a cell "talk" to others by making physical contact.

Without an automated approach, the job of looking -- one by one -- for all the physical contacts among the protein products of the thousands of genes in a yeast cell would be a painstakingly slow process.

Even though the researchers detected only a fraction -- roughly a thousand -- of such physical contacts, the impact of the new approach is expected to be significant.

"Scientists all over the world working in yeast will be able to use this information," said Dr. Stanley Fields of the University of Washington, one of the paper's senior authors. The work appears in the February 10 issue of Nature, and is featured on the cover of the journal.

Baker's yeast -- known to researchers as Saccharomyces cerevisiae -- is a laboratory darling to thousands of scientists who probe mysteries of biology, a large number of which are germane to understanding human health and disease. Though primitive, yeast cells share an extraordinary number of important similarities with more highly evolved species, including humans.

"Listening in" on which proteins physically talk to other proteins is a critical task for researchers, since all cells rely on extensive and ongoing molecular discussions to carry out life's functions -- everything from breathing to memory.

Other scientists have developed powerful approaches to determine which of the thousands of genes are "turned on" in a particular cell, but they haven't had a "guide book" to tell them which gene products likely touch each other.

"Now they have one," said Dr. James Anderson, a molecular biologist at the National Institute of General Medical Sciences, one of the NIH components that funded the study. "Dr. Fields' work adds an essential piece to the puzzle posed by genetic information that appears to be an enormous jumble of letters and words," he added.

Imagine, for instance, visiting a library full of books that you couldn't read. In a sense, this is the scientific dilemma facing biologists across the globe. Researchers have in hand boatloads of genetic information--billions of DNA letters that spell out the instructions for life in organisms as diverse as yeast, worms, flies, and humans. The problem is that, to a great degree, no one knows what all these genes do. And even in the cases where scientists do know, even more puzzling is how cell parts communicate with each other, often through physical contact.

Dr. Fields' team and their CuraGen colleagues accomplished the work by automating state-of-the-art, but commonly used, molecular biological techniques. The researchers used two separate approaches to attack the problem. Each was an automated strategy in which a test cell only survives if it contains proteins that touch each other.

According to Dr. Fields, the key element underpinning their current research tour de force was the availability of the entire DNA sequence of the genome of baker's yeast and the ability to recognize the genes. When the complete sequence of the human genome is available to researchers in the next couple of years, Dr. Fields predicts, a similar strategy will be possible using human cells.

"It's just a matter of scaling up," he said.

In addition to NIGMS, NIH's National Center for Research Resources (NCRR) also provided funding for the work, along with the Howard Hughes Medical Institute and the Merck Genome Research Institute.


Story Source:

The above story is based on materials provided by NIH-National Institute Of General Medical Sciences. Note: Materials may be edited for content and length.


Cite This Page:

NIH-National Institute Of General Medical Sciences. "Robots Eavesdrop On Cellular Discussions." ScienceDaily. ScienceDaily, 10 February 2000. <www.sciencedaily.com/releases/2000/02/000210071438.htm>.
NIH-National Institute Of General Medical Sciences. (2000, February 10). Robots Eavesdrop On Cellular Discussions. ScienceDaily. Retrieved July 23, 2014 from www.sciencedaily.com/releases/2000/02/000210071438.htm
NIH-National Institute Of General Medical Sciences. "Robots Eavesdrop On Cellular Discussions." ScienceDaily. www.sciencedaily.com/releases/2000/02/000210071438.htm (accessed July 23, 2014).

Share This




More Health & Medicine News

Wednesday, July 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Courts Conflicted Over Healthcare Law

Courts Conflicted Over Healthcare Law

AP (July 22, 2014) Two federal appeals courts issued conflicting rulings Tuesday on the legality of the federally-run healthcare exchange that operates in 36 states. (July 22) Video provided by AP
Powered by NewsLook.com
Why Do People Believe We Only Use 10 Percent Of Our Brains?

Why Do People Believe We Only Use 10 Percent Of Our Brains?

Newsy (July 22, 2014) The new sci-fi thriller "Lucy" is making people question whether we really use all our brainpower. But, as scientists have insisted for years, we do. Video provided by Newsy
Powered by NewsLook.com
Scientists Find New Way To Make Human Platelets

Scientists Find New Way To Make Human Platelets

Newsy (July 22, 2014) Boston scientists have discovered a new way to create fully functioning human platelets using a bioreactor and human stem cells. Video provided by Newsy
Powered by NewsLook.com
Gilead's $1000-a-Pill Drug Could Cure Hep C in HIV-Positive People

Gilead's $1000-a-Pill Drug Could Cure Hep C in HIV-Positive People

TheStreet (July 21, 2014) New research shows Gilead Science's drug Sovaldi helps in curing hepatitis C in those who suffer from HIV. In a medical study, the combination of Gilead's Hep C drug with anti-viral drug Ribavirin cured 76% of HIV-positive patients suffering from the most common hepatitis C strain. Hepatitis C and related complications have been a top cause of death in HIV-positive patients. Typical medication used to treat the disease, including interferon proteins, tended to react badly with HIV drugs. However, Sovaldi's %1,000-a-pill price tag could limit the number of patients able to access the treatment. TheStreet's Keris Lahiff reports from New York. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins