Featured Research

from universities, journals, and other organizations

Scientists Discover How To Grow Cells That Suppress Immune Responses

Date:
January 23, 2003
Source:
Washington University School Of Medicine
Summary:
Researchers at Washington University School of Medicine in St. Louis have discovered how to grow a little-understood type of human immune cell. The cells, known as T-regulatory cells type 1 (Tr1), are thought to turn off unnecessary immune reactions and to block the action of immune cells that otherwise would attack the body and cause dangerous inflammation.

St. Louis, Jan. 22, 2003 — Researchers at Washington University School of Medicine in St. Louis have discovered how to grow a little-understood type of human immune cell. The cells, known as T-regulatory cells type 1 (Tr1), are thought to turn off unnecessary immune reactions and to block the action of immune cells that otherwise would attack the body and cause dangerous inflammation. The findings are reported in the Jan. 23 issue of the journal Nature.

“T-regulator cells have become an important area of immunology,” says John P. Atkinson, M.D., the Samuel B. Grant Professor of Medicine and professor of molecular microbiology, who led the study. “But no one has known how to grow them in the laboratory. These findings will let that promising research move forward.”

Research using laboratory-grown Tr1 cells could lead to new treatments for autoimmune diseases such as lupus and rheumatoid arthritis and for organ rejection following transplantation, and could provide a better understanding of measles, meningitis and other infectious diseases.

“We now can take a blood sample from someone’s arm, culture selected cells from that sample and a few days later have a nice population of T-regulatory cells,” says first author Claudia Kemper, Ph.D., a postdoctoral fellow in Atkinson’s laboratory. “To be able to manipulate the activity of Tr1 cells for future therapeutic use relies heavily on knowing the factors required for their differentiation and function.”

In 1985, Atkinson’s team discovered a protein known as CD46 on cell surfaces. Usually this protein protects cells from being destroyed by a component of the immune system known as complement.

In this study, Kemper and her colleagues found that stimulating CD46 and a second cell-surface molecule known as T-cell receptor caused certain kinds of immune cells called T lymphocytes to grow, divide and give off a substance known as interleukin-10 (IL-10).

The team established the finding by growing Tr1 cells in culture dishes for several days, drawing off some of the fluid bathing the cells and adding that fluid to other dishes containing activated, proliferating infection-fighting T cells. The fluid, which contained IL-10 produced by the Tr1 cells, shut down the growth and activity of the T cells.

“That was a very good day,” says Kemper. “IL-10 is the classic substance that suppresses the action and proliferation of other immune cells.”

The investigators next want to study how CD46 triggers production of IL-10 and to better define the population of cells that give rise to Tr1 cells. They also want to explore how viruses, including those that cause measles, meningitis and herpes, interact with CD46.

“It’s tempting to think that these pathogens dock with CD46 because it causes some cells to produce IL-10, which would suppress the action of nearby immune cells and help the pathogen survive,” says Kemper. “We can investigate such questions because we can now grow these cells in the laboratory.”

Kemper C, Chan AC, Green JM, Brett KA, Murphy KM, Atkinson JP. Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype. Nature, Jan. 23, 2003.

Funding from the National Institute of Allergy and Infectious Diseases supported this research.

The full-time and volunteer faculty of Washington University School of Medicine are the physicians and surgeons of Barnes-Jewish and St. Louis Children's hospitals. The School of Medicine is one of the leading medical research, teaching and patient-care institutions in the nation. Through its affiliations with Barnes-Jewish and St. Louis Children's hospitals, the School of Medicine is linked to BJC HealthCare.


Story Source:

The above story is based on materials provided by Washington University School Of Medicine. Note: Materials may be edited for content and length.


Cite This Page:

Washington University School Of Medicine. "Scientists Discover How To Grow Cells That Suppress Immune Responses." ScienceDaily. ScienceDaily, 23 January 2003. <www.sciencedaily.com/releases/2003/01/030123073553.htm>.
Washington University School Of Medicine. (2003, January 23). Scientists Discover How To Grow Cells That Suppress Immune Responses. ScienceDaily. Retrieved July 29, 2014 from www.sciencedaily.com/releases/2003/01/030123073553.htm
Washington University School Of Medicine. "Scientists Discover How To Grow Cells That Suppress Immune Responses." ScienceDaily. www.sciencedaily.com/releases/2003/01/030123073553.htm (accessed July 29, 2014).

Share This




More Health & Medicine News

Tuesday, July 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deadly Ebola Virus Threatens West Africa

Deadly Ebola Virus Threatens West Africa

AP (July 28, 2014) West African nations and international health organizations are working to contain the largest Ebola outbreak in history. It's one of the deadliest diseases known to man, but the CDC says it's unlikely to spread in the U.S. (July 28) Video provided by AP
Powered by NewsLook.com
$15B Deal on Vets' Health Care Reached

$15B Deal on Vets' Health Care Reached

AP (July 28, 2014) A bipartisan deal to improve veterans health care would authorize at least $15 billion in emergency spending to fix a veterans program scandalized by long patient wait times and falsified records. (July 28) Video provided by AP
Powered by NewsLook.com
Two Americans Contract Ebola in Liberia

Two Americans Contract Ebola in Liberia

Reuters - US Online Video (July 28, 2014) Two American aid workers in Liberia test positive for Ebola while working to combat the deadliest outbreak of the virus ever. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins