Featured Research

from universities, journals, and other organizations

Scientists Take A Step Nearer To Creating An Artificial Egg Using A Somatic Cell

Date:
July 2, 2003
Source:
European Society For Human Reproduction And Embryology
Summary:
Scientists believe that they are an important step nearer to success in creating an artificial egg from the combination of the nucleus of a somatic cell and an oocyte which has had its DNA-carrying nucleus removed, a conference of international fertility experts heard.

Madrid, Spain -- Scientists believe that they are an important step nearer to success in creating an artificial egg from the combination of the nucleus of a somatic cell and an oocyte which has had its DNA-carrying nucleus removed, a conference of international fertility experts heard today (July 1).

Dr Peter Nagy, from Reproductive Biology Associates, Atlanta, collaborating with the University of Connecticut, USA, told the European Society of Human Reproduction and Embryology annual conference that former approaches to haploidisation[1] using a fully mature oocyte and a resting (interphase) somatic cell had caused misaligned chromosomes during cell division. However, he was confident from his team's latest experiments that this difficulty could be overcome, even though their new approach also ran into some problems.

"We decided to initiate haploidisation at an earlier stage in the oocyte's cell cycle, when it was still immature, but this time using a somatic cell in its active (metaphase or G2/M) stage. Essentially, we took the control of the first nuclear division away from the oocyte and gave it to the somatic cell," he said.

The US-Brazilian research team[2] , working with mouse cells, removed the nucleus of the immature oocyte, then transformed the somatic cell from its diploid (46 chromosome or 2n) stage to its next (4n) stage and transferred it to the immature enucleated oocyte (ooplast).

"What we expected by doing this was that the DNA in the somatic cell would condense into chromosomes inside the somatic cell – not in the ooplast – and that the somatic cell would direct the chromosome alignment and initial spindle formation, which would then be normal. The nucleus of the somatic cell, at its second stage of division and correctly assembled, would then undergo chromosome segregation in the ooplast, resulting in twice its diploid nuclear content during in-vitro maturation. As a result of an artificial activation, a second round of chromosome segregation provides the haploid (23 chromosome) normal oocyte content. This is a novel strategy that cannot be used with a mature ooplast because mature ooplasts can support only one round of chromosome segregation."

However, the researchers found that there were still some misaligned chromosomes and problems with the integrity of the spindle – the chromosomes' 'holding' mechanism. But, they are confident that these will be overcome.

"This initial set of experiments shows that it is possible to induce haploidisation with our approach," said Dr Nagy. "This is the first time that this has been tried so we are still learning. Now we have to check how frequently the chromosomal problems occur and whether there is an easy solution or whether it is a fundamental difficulty."

But, even in a worst case scenario, he said, it does not mean that they were back to the drawing board because his team was already developing new techniques to overcome the problem.

"I'm really confident – not simply optimistic – that haploidisation will work and if everything goes well we will be able to obtain artificial gametes in one or two years. Even if we encounter more problems it should still be possible within three to five years."

Haploidisation is not cloning because it is the production of a reconstituted egg (which can then be fertilised by the sperm) in a situation where a woman has no eggs of her own. One of the woman's own somatic cells would be the source of the chromosome-carrying nucleus, which would be transferred into a donated 'shelled-out' oocyte.

###

Notes:

[1] Haploid: a cell with only one set of chromosomes – in humans 23. Only the egg and sperm are haploid.

[2] University of Connecticut, Animal Science, Storrs, USA; Reproductive Biology Associates, Atlanta, USA; Clinical e Centro de Pesquisa em Reproduηγo Humana Roger Abdelmassih, Sγo Paulo, Brazil.


Story Source:

The above story is based on materials provided by European Society For Human Reproduction And Embryology. Note: Materials may be edited for content and length.


Cite This Page:

European Society For Human Reproduction And Embryology. "Scientists Take A Step Nearer To Creating An Artificial Egg Using A Somatic Cell." ScienceDaily. ScienceDaily, 2 July 2003. <www.sciencedaily.com/releases/2003/07/030701220315.htm>.
European Society For Human Reproduction And Embryology. (2003, July 2). Scientists Take A Step Nearer To Creating An Artificial Egg Using A Somatic Cell. ScienceDaily. Retrieved July 23, 2014 from www.sciencedaily.com/releases/2003/07/030701220315.htm
European Society For Human Reproduction And Embryology. "Scientists Take A Step Nearer To Creating An Artificial Egg Using A Somatic Cell." ScienceDaily. www.sciencedaily.com/releases/2003/07/030701220315.htm (accessed July 23, 2014).

Share This




More Plants & Animals News

Wednesday, July 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Stone Fruit Listeria Scare Causes Sweeping Recall

Stone Fruit Listeria Scare Causes Sweeping Recall

Newsy (July 22, 2014) — The Wawona Packing Company has issued a voluntary recall on the stone fruit it distributes due to a possible Listeria outbreak. Video provided by Newsy
Powered by NewsLook.com
Michigan Plant's Goal: Flower and Die

Michigan Plant's Goal: Flower and Die

AP (July 22, 2014) — An 80-year-old agave plant, which is blooming for the first and only time at a University of Michigan conservatory, will die when it's done (July 22) Video provided by AP
Powered by NewsLook.com
Huge Schizophrenia Study Finds Dozens Of New Genetic Causes

Huge Schizophrenia Study Finds Dozens Of New Genetic Causes

Newsy (July 22, 2014) — The 83 new genetic markers could open dozens of new avenues for schizophrenia treatment research. Video provided by Newsy
Powered by NewsLook.com
CDC Head Concerned About a Post-Antibiotic Era

CDC Head Concerned About a Post-Antibiotic Era

AP (July 22, 2014) — Sounding alarms about the growing threat of antibiotic resistance, CDC Director Tom Frieden warned Tuesday if the global community does not confront the problem soon, the world will be living in a devastating post-antibiotic era. (July 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins