Featured Research

from universities, journals, and other organizations

Developing Tools For Reliable 'Gene Chip' Measurements

Date:
May 28, 2004
Source:
National Institute Of Standards And Technology
Summary:
Microarrays, sometimes called "gene chip" devices, enable researchers to monitor the activities of thousands of genes from a single tissue sample simultaneously, identifying patterns that may be novel indicators of disease status.

Microarrays, sometimes called "gene chip" devices, enable researchers to monitor the activities of thousands of genes from a single tissue sample simultaneously, identifying patterns that may be novel indicators of disease status. But generating consistent, verifiable results is difficult because of a lack of standards to validate these analyses, scientists from the National Institute of Standards and Technology (NIST) and collaborators warn in the May 20 online issue of Clinical Chemistry.

Microarrays are keychain-sized devices with as many as several million tiny spots, each of which examines genes of interest simultaneously using minute sample volumes. This highly sensitive technology is relatively new, and standard procedures to ensure the reliability and comparability of results are only beginning to emerge. For instance, results can change as a result of differences in how tissues are collected and processed; variations in how the molecules are counted, attached to substrates and labeled for detection; deviations from recommended protocols by lab personnel; and malfunctioning or miscalibrated equipment. Such variations need to be controlled before this technology can be used reliably in clinical settings and in devices requiring regulatory approval, according to the paper.

As a first step toward addressing reliability issues, a consortium co-led by NIST and industry is developing standards that will satisfy needs identified at a 2003 workshop. At the workshop, organized and hosted by NIST, leaders in the microarray field from industry, government and universities recommended the development of a well-characterized set of ribonucleic acid (RNA) molecules whose identity and concentration are known. RNA is an important product of gene activity. Users will be able to validate the results of gene chip analyses by adding such a reference material to their samples and comparing the measured values to what would be expected for them. Such a reference material also will enable technology developers and researchers to assess the performance of their assays.

The paper was co-authored by scientists from Genomic Health, Inc., Agilent Technologies, the U.S. Food and Drug Administration, and The Institute for Genome Research.


Story Source:

The above story is based on materials provided by National Institute Of Standards And Technology. Note: Materials may be edited for content and length.


Cite This Page:

National Institute Of Standards And Technology. "Developing Tools For Reliable 'Gene Chip' Measurements." ScienceDaily. ScienceDaily, 28 May 2004. <www.sciencedaily.com/releases/2004/05/040525060054.htm>.
National Institute Of Standards And Technology. (2004, May 28). Developing Tools For Reliable 'Gene Chip' Measurements. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/2004/05/040525060054.htm
National Institute Of Standards And Technology. "Developing Tools For Reliable 'Gene Chip' Measurements." ScienceDaily. www.sciencedaily.com/releases/2004/05/040525060054.htm (accessed October 21, 2014).

Share This



More Matter & Energy News

Tuesday, October 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Thanks, Marty McFly! Hoverboards Could Be Coming In 2015

Thanks, Marty McFly! Hoverboards Could Be Coming In 2015

Newsy (Oct. 21, 2014) If you've ever watched "Back to the Future Part II" and wanted to get your hands on a hoverboard, well, you might soon be in luck. Video provided by Newsy
Powered by NewsLook.com
Robots to Fly Planes Where Humans Can't

Robots to Fly Planes Where Humans Can't

Reuters - Innovations Video Online (Oct. 21, 2014) Researchers in South Korea are developing a robotic pilot that could potentially replace humans in the cockpit. Unlike drones and autopilot programs which are configured for specific aircraft, the robots' humanoid design will allow it to fly any type of plane with no additional sensors. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com
Graphene Paint Offers Rust-Free Future

Graphene Paint Offers Rust-Free Future

Reuters - Innovations Video Online (Oct. 21, 2014) British scientists have developed a prototype graphene paint that can make coatings which are resistant to liquids, gases, and chemicals. The team says the paint could have a variety of uses, from stopping ships rusting to keeping food fresher for longer. Jim Drury reports. Video provided by Reuters
Powered by NewsLook.com
Portable Breathalyzer Gets You Home Safely

Portable Breathalyzer Gets You Home Safely

Buzz60 (Oct. 21, 2014) Breeze, a portable breathalyzer, gets you home safely by instantly showing your blood alcohol content, and with one tap, lets you call an Uber, a cab or a friend from your contact list to pick you up. Sean Dowling (@SeanDowlingTV) has the details. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins