Featured Research

from universities, journals, and other organizations

Major Advance Made On DNA Structure

Date:
May 4, 2005
Source:
Oregon State University
Summary:
Oregon State University researchers have made significant new advances in determining the structure of all possible DNA sequences -- a discovery that in one sense takes up where Watson and Crick left off, after outlining in 1953 the double-helical structure of this biological blueprint for life. The work may be a milestone in DNA structural biology.

CORVALLIS, Ore. -- Oregon State University researchers have made significant new advances in determining the structure of all possible DNA sequences -- a discovery that in one sense takes up where Watson and Crick left off, after outlining in 1953 the double-helical structure of this biological blueprint for life.

Related Articles


One of the fundamental problems in biochemistry is to predict the structure of a molecule from its sequence -- this has been referred to as the "Holy Grail" of protein chemistry.

Today, the OSU scientists announced in the Proceedings of the National Academy of Sciences that they have used X-ray crystallography to determine the three-dimensional structures of nearly all the possible sequences of a macromolecule, and thereby create a map of DNA structure.

As work of this type expands, it should be fundamentally important in explaining the actual biological function of genes - in particular, such issues as genetic "expression," DNA mutation and repair, and why some DNA structures are inherently prone to damage and mutation. Understanding DNA structure, the scientists say, is just as necessary as knowing gene sequence. The human genome project, with its detailed explanation of the genetic sequence of the entire human genome, is one side of the coin. The other side is understanding how the three-dimensional structure of different types of DNA are defined by those sequences, and, ultimately, how that defines biological function.

"There can be 400 million nucleotides in a human chromosome, but only about 10 percent of them actually code for genes," said Pui Shing Ho, professor and chair of the OSU Department of Biochemistry and Biophysics. "The other 90 percent of the nucleotides may play different roles, such as regulating gene expression, and they often do that through variations in DNA structure."

"Now, for the first time, we're really starting to see what the genome looks like in three dimensional reality, not just what the sequence of genes is," Ho said. "DNA is much more than just a string of letters, it's an actual structure that we have to explore if we ever hope to understand biological function. This is a significant step forward, a milestone in DNA structural biology."

In the early 1950s, two researchers at Cambridge University -- James Watson and Francis Crick -- made pioneering discoveries by proposing the double-helix structure of DNA, along with another research group in England about the same time. They later received the Nobel Prize for this breakthrough, which has been called the most important biological work of the past century and revolutionized the study of biochemistry. Some of the other early and profoundly important work in protein chemistry was done by Linus Pauling, an OSU alumnus and himself the recipient of two Nobel Prizes.

However, Watson and Crick actually identified only one structure of DNA, called B-DNA, when in fact there are many others -- one of which was discovered and another whose structure was solved at OSU in recent years -- that all have different effects on genetic function.

Aside from the genetic sequence that DNA encodes, the structure of the DNA itself can have profound biological effects, scientists now understand. Until now, there has been no reliable method to identify DNA structure from sequence, and learn more about its effects on biological function.

In their studies, the OSU scientists used X-ray examination of crystalline DNA to reconstruct exactly what the DNA looks like at the atomic level. By determining 63 of the 64 possible DNA sequences, they were able to ultimately determine the physical structure of the underlying DNA for all different types of sequences. Another important part of this study is the finding that the process of DNA crystallization does not distort its structure.

"Essentially, this is a proof of concept, a demonstration that this approach to studying DNA structure will work, and can ultimately be used to help understand biology," Ho said.

For instance, one of the unusual DNA structures called a Holliday junction, whose structure was co-solved at OSU about five years ago, apparently plays a key role in DNA's ability to repair itself -- a vital biological function.

A more fundamental understanding of DNA structure and its relationship to genetic sequences, researchers say, helps set the stage for applied advances in biology, biomedicine, genetic engineering, nanotechnology and other fields.

The recent work was supported by grants from the National Institutes of Health and the National Science Foundation.



Story Source:

The above story is based on materials provided by Oregon State University. Note: Materials may be edited for content and length.


Cite This Page:

Oregon State University. "Major Advance Made On DNA Structure." ScienceDaily. ScienceDaily, 4 May 2005. <www.sciencedaily.com/releases/2005/05/050504003217.htm>.
Oregon State University. (2005, May 4). Major Advance Made On DNA Structure. ScienceDaily. Retrieved November 24, 2014 from www.sciencedaily.com/releases/2005/05/050504003217.htm
Oregon State University. "Major Advance Made On DNA Structure." ScienceDaily. www.sciencedaily.com/releases/2005/05/050504003217.htm (accessed November 24, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Monday, November 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Winter Can Cause Depression — Here's How To Combat It

Winter Can Cause Depression — Here's How To Combat It

Newsy (Nov. 23, 2014) Millions of American suffer from seasonal depression every year. It can lead to adverse health effects, but there are ways to ease symptoms. Video provided by Newsy
Powered by NewsLook.com
Ebola-Hit Sierra Leone's Late Cocoa Leaves Bitter Taste

Ebola-Hit Sierra Leone's Late Cocoa Leaves Bitter Taste

AFP (Nov. 23, 2014) The arable district of Kenema in Sierra Leone -- at the centre of the Ebola outbreak in May -- has been under quarantine for three months as the cocoa harvest comes in. Duration: 01:32 Video provided by AFP
Powered by NewsLook.com
Don't Fall For Flu Shot Myths

Don't Fall For Flu Shot Myths

Newsy (Nov. 23, 2014) Misconceptions abound when it comes to your annual flu shot. Medical experts say most people older than 6 months should get the shot. Video provided by Newsy
Powered by NewsLook.com
Indians Muck in for Cleaner Communities

Indians Muck in for Cleaner Communities

AFP (Nov. 22, 2014) India's government is urging all citizens to come together in a mass movement to clean the nation -- but will people heed the call? Duration: 02:39 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins