Featured Research

from universities, journals, and other organizations

Antibody Retards Growth And Induces Death In Liver Cancer Cells

Date:
July 13, 2007
Source:
University of Pittsburgh Schools of the Health Sciences
Summary:
Researchers report a significant new advance in the search for an effective treatment for human liver cancer. Using a newly available monoclonal antibody, they demonstrated dramatic reductions in tumor cell proliferation and survival in human and mouse hepatocellular cancer cell lines. This finding has significant implications not only for the treatment of liver cancer but for a number of different types of cancer.

Researchers from the University of Pittsburgh School of Medicine report a significant new advance in the search for an effective treatment for human liver cancer in the July issue of Molecular Cancer Therapeutics.

Using a newly available monoclonal antibody, they demonstrated significant reductions in tumor cell proliferation and survival in human and mouse hepatocellular cancer (HCC) cell lines. According to the researchers, this finding has significant implications not only for the treatment of liver cancer but for a number of different types of cancer.

Most cases of HCC are secondary to either a viral hepatitis infection or cirrhosis of the liver. Despite recent advances, it remains a disease of grim prognosis due to the poorly understood mechanism of how the disease originates and spreads. Most patients live only a short time after diagnosis.

Based on previous studies showing that some pathways that were previously thought to be active only during fetal liver development, particularly the class III receptor tyrosine kinase (RTK) family pathway, became highly active again in the liver of HCC patients, Satdarshan P. Singh Monga, M.D., associate professor, division of cellular and molecular pathology and colleagues at the University of Pittsburgh School of Medicine, obtained rat and human liver cancer cell lines and analyzed them for level of expression of an RTK protein known as platelet-derived growth factor receptor-alpha, or PDGFRá. The investigators also analyzed the cells for their level of activation of the PDGFRá gene.

At an early fetal stage of liver development in the mouse, the investigators found that the level of expression of PDGFRá was 37 times higher compared to later stages of development in the adult mouse liver. They also found significantly higher levels of PDGFRá in rat and human liver cancer cell lines as compared to normal cells in culture.

Dr. Monga's group then treated human and mouse liver cancer cell lines with a monoclonal antibody targeted against PDGFRá. It resulted in a significant decrease in tumor cell proliferation and a marked increase in tumor cell death. In fact, all tumor cell lines experienced significant decreases in proliferation in response to the monoclonal antibody and there was a 4- to 18-fold increase in programmed cell death, or apoptosis, among the cancer cell lines compared to normal control cells.

According to Dr. Monga, these results suggest that PDGFRá offers an important new therapeutic target for the treatment of HCC.

"We are very excited because this is the first targeted therapy for liver cancer. Other therapies have some modest benefits, but no one knows exactly how they work. We now have identified a pathway that appears to be overly active in more than 70 percent of the cancers we examined and, when targeted, leads to significant reduction in tumor cell proliferation and survival," said Dr. Monga.

More importantly, targeting the PDGFRá pathway in liver cancer cells does not appear to affect normal liver cells, making the treatment relatively non-toxic. "Normally, regenerating liver cells are not exclusively dependent on this pathway, and it is not overly active in other types of cells. So this monoclonal antibody is a highly targeted treatment for this disease," he added.

Furthermore, because high expression of PDGFRá has been detected in a variety of tumors, such as skin cancer, brain tumors, gastrointestinal tumors, prostate tumors, ovarian cancer and leukemia, Dr. Monga believes these findings could have much broader applications.

This research was funded by grants from the American Cancer Society and the National Institutes of Health as well as the Cleveland Foundation and the Rango's Fund for Enhancement of Pathology Research.

In addition to Dr. Monga, other researchers involved in the study included Peggy Stock, Xinping Tan and Amanda Micsenyi, all in the department of pathology, University of Pittsburgh School of Medicine; Dulabh Monga, department of human oncology, Allegheny General Hospital, Pittsburgh; and Nick Loizos, ImClone Systems, Inc., New York.


Story Source:

The above story is based on materials provided by University of Pittsburgh Schools of the Health Sciences. Note: Materials may be edited for content and length.


Cite This Page:

University of Pittsburgh Schools of the Health Sciences. "Antibody Retards Growth And Induces Death In Liver Cancer Cells." ScienceDaily. ScienceDaily, 13 July 2007. <www.sciencedaily.com/releases/2007/07/070711134513.htm>.
University of Pittsburgh Schools of the Health Sciences. (2007, July 13). Antibody Retards Growth And Induces Death In Liver Cancer Cells. ScienceDaily. Retrieved September 18, 2014 from www.sciencedaily.com/releases/2007/07/070711134513.htm
University of Pittsburgh Schools of the Health Sciences. "Antibody Retards Growth And Induces Death In Liver Cancer Cells." ScienceDaily. www.sciencedaily.com/releases/2007/07/070711134513.htm (accessed September 18, 2014).

Share This



More Health & Medicine News

Thursday, September 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Artificial Sweetener Could Promote Diabetes

Artificial Sweetener Could Promote Diabetes

Newsy (Sep. 17, 2014) — Doctors once thought artificial sweeteners lacked the health risks of sugar, but a new study says they can impact blood sugar levels the same way. Video provided by Newsy
Powered by NewsLook.com
Ebola Vaccine Trial Gets Underway at Oxford University

Ebola Vaccine Trial Gets Underway at Oxford University

AFP (Sep. 17, 2014) — A healthy British volunteer is to become the first person to receive a new vaccine for the Ebola virus after US President Barack Obama called for action against the epidemic and warned it was "spiralling out of control." Duration: 01:02 Video provided by AFP
Powered by NewsLook.com
Obesity Rates Steady Even As Americans' Waistlines Expand

Obesity Rates Steady Even As Americans' Waistlines Expand

Newsy (Sep. 17, 2014) — Researchers are puzzled as to why obesity rates remain relatively stable as average waistlines continue to expand. Video provided by Newsy
Powered by NewsLook.com
President To Send 3,000 Military Personnel To Fight Ebola

President To Send 3,000 Military Personnel To Fight Ebola

Newsy (Sep. 16, 2014) — President Obama is expected to send 3,000 troops to West Africa as part of the effort to contain Ebola's spread. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins