Featured Research

from universities, journals, and other organizations

Key Sperm-binding Proteins Cloned

Date:
March 18, 2009
Source:
University of Montreal
Summary:
New treatments for infertility could be closer to reality. According to a study published in the journal Molecular Human Reproduction, for the first time researchers have cloned, produced and purified a protein important for sperm maturation, termed Binder of Sperm, which may have implications for both fertility treatments and new methods of male contraception.

New treatments for infertility could be closer to reality, thanks to a discovery from scientists at the University of Montreal and Maisonneuve-Rosemont Hospital Research Center. According to a study published in the journal Molecular Human Reproduction, the researchers have become the first to clone, produce and purify a protein important for sperm maturation, termed Binder of Sperm, which may have implications for both fertility treatments and new methods of male contraception
Credit: University of Montreal and Maisonneuve-Rosemont Hospital Research Center

New treatments for infertility could be closer to reality, thanks to a discovery from scientists at the Université de Montréal and Maisonneuve-Rosemont Hospital Research Centre. According to a study published in the journal Molecular Human Reproduction, the researchers have become the first to clone, produce and purify a protein important for sperm maturation, termed Binder of Sperm (BSP), which may have implications for both fertility treatments and new methods of male contraception.

Related Articles


"We have previously isolated and characterized BSPs from many species, such as bulls and boars," says Dr. Puttaswamy Manjunath, senior author and a professor in the departments of medicine and of biochemistry at the Université de Montréal and a member of the Maisonneuve-Rosemont Hospital Research Centre.

"We know from these studies that if this protein is missing or defective in these species, fertility is compromised. We believe that BSP is equally important in humans."

An elusive protein

Dr. Manjunath and colleagues have tried to isolate human BSPs for more than 10 years. In most mammals, these proteins are typically produced by the seminal vesicles and added to sperm at ejaculation. Yet this is not the case for humans, primates and rodents. According to Dr.Manjunath and his team, these species produce small amounts of BSPs only in the epididymis, a duct that connects the testes to the urethra.

"For a few years, we were looking in the wrong place," says Dr. Manjunath. "In addition, the minute quantities of BSP produced in humans has made it impossible to isolate and characterize."

Cloning leads to purification

Dr. Manjunath and his team went back to the basics. Using molecular biology technique they cloned the gene (DNA) that encodes human BSP. Through cloning, they were able to produce and purify this protein.

"After considerable troubleshooting, we were able to produce functional human BSP. Our next steps are to confirm its biological role in human fertility," says Dr. Manjunath.

Role of BSPs in other animals

Following ejaculation, sperm undergo a complex series of modifications inside the female reproductive tract. The changes sperm undergo during this process include redistribution of surface proteins, loss of sperm membrane lipids and increased sperm movement. A family of sperm-binding proteins (BSPs) secreted by the seminal vesicles has been shown to be essential for sperm maturation in female reproductive tracts of cows, sheep, pigs and other hoofed animals.


Story Source:

The above story is based on materials provided by University of Montreal. Note: Materials may be edited for content and length.


Journal Reference:

  1. Lefebvre et al. Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1. Molecular Human Reproduction, 2008; 15 (2): 105 DOI: 10.1093/molehr/gan077

Cite This Page:

University of Montreal. "Key Sperm-binding Proteins Cloned." ScienceDaily. ScienceDaily, 18 March 2009. <www.sciencedaily.com/releases/2009/03/090317113816.htm>.
University of Montreal. (2009, March 18). Key Sperm-binding Proteins Cloned. ScienceDaily. Retrieved January 25, 2015 from www.sciencedaily.com/releases/2009/03/090317113816.htm
University of Montreal. "Key Sperm-binding Proteins Cloned." ScienceDaily. www.sciencedaily.com/releases/2009/03/090317113816.htm (accessed January 25, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Sunday, January 25, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Mistakes Should Serve a Lesson Says WHO

Ebola Mistakes Should Serve a Lesson Says WHO

AFP (Jan. 25, 2015) — The World Health Organization&apos;s chief on Sunday admitted the UN agency had been caught napping on Ebola, saying it should serve a lesson to avoid similar mistakes in future. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com
Disneyland Measles Outbreak Spreads To 5 States

Disneyland Measles Outbreak Spreads To 5 States

Newsy (Jan. 24, 2015) — Much of the Disneyland measles outbreak is being blamed on the anti-vaccination movement. The CDC encourages just about everyone get immunized. Video provided by Newsy
Powered by NewsLook.com
Growing Measles Outbreak Worries Calif. Parents

Growing Measles Outbreak Worries Calif. Parents

AP (Jan. 23, 2015) — Public health officials are rushing to contain a measles outbreak that has sickened 70 people across 6 states and Mexico. The AP&apos;s Raquel Maria Dillon has more. (Jan. 23) Video provided by AP
Powered by NewsLook.com
Smart Wristband to Shock Away Bad Habits

Smart Wristband to Shock Away Bad Habits

Reuters - Innovations Video Online (Jan. 23, 2015) — A Boston start-up is developing a wristband they say will help users break bad habits by jolting them with an electric shock. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins