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Sexually Transmitted Infections: Transistors Used To Detect Fungus Candida Albicans

Date:
May 11, 2009
Source:
Plataforma SINC
Summary:
Scientists have developed a biosensor that makes it possible to detect small quantities of the fungus Candida albicans, the cause of common sexually-transmitted infections, within just an hour. The technique involves the use of transistors, which incorporate specific antibodies able to recognize yeast, and carbon nanotubes to generate a measurable electrical signal.

The Nanosensors group from the URV has created a biosensor, an electrical and biological device, which is able to selectively detect the Candida albicans yeast in very small quantities of only 50 cfu/ml (colony-forming units per millilitre).
Credit: Copyright SINC/Villamizar et al

The Nanosensors group from the Universidad Rovira i Virgili has created a biosensor, an electrical and biological device, which is able to selectively detect the Candida albicans yeast in very small quantities of only 50 cfu/ml (colony-forming units per millilitre).

"The technique uses field-effect transistors (electronic devices that contain an electrode source and a draining electrode connected to a transducer) based on carbon nanotubes and with Candida albicans-specific antibodies", Raquel A. Villamizar, lead author of the study said.

The Candida samples, which can be obtained from blood, serum or vaginal secretions, are placed directly on the biosensor, where the interaction between antigens and antibodies changes the electric current of the devices. This change is recorded and makes it possible to measure the amount of yeast present in a sample.

"Thanks to the extraordinary charge transference properties of the carbon nanotubes, the fungus detection process is direct, fast, and does not require the use of any marker", remarks Villamizar, who is co-author of a study that provides details of the biosensor and was published recently in the journal Sensors and Actuators B: Chemical.

To date, conventional diagnosis of Candida has been carried out using microbial cultures, serological tests, PCR molecular biology techniques (polymerase chain reactions used to amplify DNA), or immunoassays such as ELISA (Enzyme Linked Inmunoabsorbent Assay).

These techniques require long analysis times and sometimes give rise to false positives and negatives. ELISA also requires the use of markers (compounds that must be added to detect the presence of yeast by fluorescence and other techniques).

The new carbon nanotubes biosensor, however, "makes it possible to improve some of the quality parameters of the traditional methods, for example the speed and simplicity of measurements, and it is an alternative tool that could be used in routine sample analysis", explains Villamizar.

The researcher adds that by using this biosensor "it will be possible in future to obtain a rapid diagnosis of infection with this pathogen, which will help to ensure administration of the correct prophylactic treatments".

The Candida albicans fungus exists naturally in the skin, mouth, the mucous membranes lining the digestive tract, and the respiratory and genitourinary systems. This yeast can cause anything from simple mycosis of the skin to complicated cases of candidiasis. It is much more commonly found in patients suffering from immunodeficiency, tumours, diabetes and lymphomas, among other diseases.


Story Source:

The above story is based on materials provided by Plataforma SINC. Note: Materials may be edited for content and length.


Journal Reference:

  1. Villamizar et al. Improved detection of Candida albicans with carbon nanotube field-effect transistors. Sensors and Actuators B Chemical, 2009; 136 (2): 451 DOI: 10.1016/j.snb.2008.10.013

Cite This Page:

Plataforma SINC. "Sexually Transmitted Infections: Transistors Used To Detect Fungus Candida Albicans." ScienceDaily. ScienceDaily, 11 May 2009. <www.sciencedaily.com/releases/2009/05/090507094308.htm>.
Plataforma SINC. (2009, May 11). Sexually Transmitted Infections: Transistors Used To Detect Fungus Candida Albicans. ScienceDaily. Retrieved September 17, 2014 from www.sciencedaily.com/releases/2009/05/090507094308.htm
Plataforma SINC. "Sexually Transmitted Infections: Transistors Used To Detect Fungus Candida Albicans." ScienceDaily. www.sciencedaily.com/releases/2009/05/090507094308.htm (accessed September 17, 2014).

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