Featured Research

from universities, journals, and other organizations

Microfluidic devices advance 3-D tissue engineering

Date:
October 9, 2010
Source:
Stevens Institute of Technology
Summary:
A new method that generates three-dimensional (3-D) tissue models for studying bacterial infection of orthopedic implants has been developed.

Bone tissue cells and bacteria within the channels were imaged with a microscope and effluent was analyzed for bacteria count.
Credit: Image courtesy of Stevens Institute of Technology

A research team, co-headed by Dr. Woo Lee and Dr. Hongjun Wang of Stevens Institute of Technology, has published a paper describing a new method that generates three-dimensional (3D) tissue models for studying bacterial infection of orthopedic implants. Dr. Joung-Hyun Lee of Stevens, and Dr. Jeffrey Kaplan of the New Jersey Dental School, are co-authors of the research. Their paper, appearing in the journal Tissue Engineering, demonstrates a physiologically relevant approach for studying infection prevention strategies and emulating antibiotic delivery using 3D bone tissues cultured in microfluidic devices.

With over 1 million hip and knee replacement procedures being performed in the United States every year, orthopedic implants have become relatively common. Despite advances in implant design, hospitals have been unable to address bacterial infection, the leading cause of failure in orthopedic implants. A significant barrier to successfully developing infection-fighting drugs or biomaterials has been the inadequacy of laboratory equipment to create clinically relevant environment with traditional in vitro methods.

The researchers seeded 0.02 mL microfluidic channels with osteoblasts and inoculated the channels with Staphylococcus epidermis bacteria, a common pathogen in orthopedic infections. Nutrient solutions were pumped through the channels at a concentration and flow rate mimicking conditions within the human body. Bone tissue cells and bacteria within the channels were imaged with a microscope and effluent was analyzed for bacteria count.

Microfluidic devices, together with finely-tuned dynamic flow settings, have the potential to provide realistic bone tissue models in clinical scenarios. As opposed to the static 2D Petri dish surfaces, microfluidic channels present a realistic environment for cells to grow and adhere in three dimensions. Dynamic fluid motion through the channels -- with solutions potentially carrying antibiotics or other novel drugs -- further mimics real-world conditions previously unrealizable in a lab setting.

The research team is comprised of Dr. Woo Lee, George Meade Bond Professor in Chemical Engineering and Materials Science; Dr. Hongjun Wang, Assistant Professor of Biomedical Engineering; Dr. Joung-Hyun Lee, Research Associate and 2010 Ph.D. graduate of Stevens; and Dr. Jeffrey Kaplan, Associate Professor in the Department of Oral Biology at the New Jersey Dental School. Dr. Joung-Hyun Lee, as the first author of this paper, used her background in microfabrication to discover the conditions for growing bone tissues in the microfluidic device channels while integrating capabilities in the laboratories of Lee, Wang, and Kaplan. This research was sponsored the Nanoscale Interdisciplinary Research Team program of the National Science Foundation (NSF). Also, Dr. Lee and Dr. Wang are principal investigators on a new grant from the NSF Biomaterials program, awarded earlier this year. In this new project, they plan to use the newly developed 3D tissue model to evaluate the efficacy of inkjet-printed infection-preventing biomaterials.

The researchers' published paper is a preliminary demonstration of dynamic microfluidic cell cultures and work continues in the lab to establish successful applications of the technology and processes.


Story Source:

The above story is based on materials provided by Stevens Institute of Technology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Joung-Hyun Lee, Hongjun Wang, Jeffrey B. Kaplan, Woo Y. Lee. Microfluidic Approach to Create Three-Dimensional Tissue Models for Biofilm-Related Infection of Orthopaedic Implants. Tissue Engineering Part C: Methods, 2010; 100830144718098 DOI: 10.1089/ten.tec.2010.0285

Cite This Page:

Stevens Institute of Technology. "Microfluidic devices advance 3-D tissue engineering." ScienceDaily. ScienceDaily, 9 October 2010. <www.sciencedaily.com/releases/2010/10/101006094053.htm>.
Stevens Institute of Technology. (2010, October 9). Microfluidic devices advance 3-D tissue engineering. ScienceDaily. Retrieved July 25, 2014 from www.sciencedaily.com/releases/2010/10/101006094053.htm
Stevens Institute of Technology. "Microfluidic devices advance 3-D tissue engineering." ScienceDaily. www.sciencedaily.com/releases/2010/10/101006094053.htm (accessed July 25, 2014).

Share This




More Matter & Energy News

Friday, July 25, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Europe's Highest Train Turns 80 in French Pyrenees

Europe's Highest Train Turns 80 in French Pyrenees

AFP (July 25, 2014) Europe's highest train, the little train of Artouste in the French Pyrenees, celebrates its 80th birthday. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
TSA Administrator on Politics and Flight Bans

TSA Administrator on Politics and Flight Bans

AP (July 24, 2014) TSA administrator, John Pistole's took part in the Aspen Security Forum 2014, where he answered questions on lifting of the ban on flights into Israel's Tel Aviv airport and whether politics played a role in lifting the ban. (July 24) Video provided by AP
Powered by NewsLook.com
Creative Makeovers for Ugly Cellphone Towers

Creative Makeovers for Ugly Cellphone Towers

AP (July 24, 2014) Mobile phone companies and communities across the country are going to new lengths to disguise those unsightly cellphone towers. From a church bell tower to a flagpole, even a pencil, some towers are trying to make a point. (July 24) Video provided by AP
Powered by NewsLook.com
Algonquin Power Goes Activist on Its Target Gas Natural

Algonquin Power Goes Activist on Its Target Gas Natural

TheStreet (July 23, 2014) When The Deal's Amanda Levin exclusively reported that Gas Natural had been talking to potential suitors, the Ohio company responded with a flat denial, claiming its board had not talked to anyone about a possible sale. Lo and behold, Canadian utility Algonquin Power and Utilities not only had approached the company, but it did it three times. Its last offer was for $13 per share as Gas Natural's was trading at a 60-day moving average of about $12.50 per share. Now Algonquin, which has a 4.9% stake in Gas Natural, has taken its case to shareholders, calling on them to back its proposals or, possibly, a change in the target's board. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins