Featured Research

from universities, journals, and other organizations

Increasing the efficiency of stem cells

Date:
October 25, 2010
Source:
The Biochemical Society
Summary:
A new technique for increasing the survival rates of human embryonic stem cells promises to revolutionize their use in drug discovery and therapy.

A new technique for increasing the survival rates of human embryonic stem cells promises to revolutionize their use in drug discovery and therapy.

Human embryonic stem cells often die during the process of isolation using enzymatic disaggregation or low-density plating, which limits their usefulness in drug discovery and basic research. Similarly, the low rate of attachment of implanted cells hinders cell therapy.

Now, researchers from the Universities of Dundee, Glasgow and Heriot-Watt University, Edinburgh, have produced a high-content assay for human embryonic stem cell survival and used this to screen a range of libraries of 'lead-like' small molecules and known bioactives.

In their paper published in the ChemBio Knowledge Environment of the Biochemical Journal, Paul Andrew and colleagues describe how they managed to find some compounds, protein kinases, which worked by targeting the ROCK2/PRK2 kinases. They also found that pinacidil, a K+-ATP channel opener, often used for preconditioning in organ transplantation, has hES cell pro-survival effects through a mechanism unrelated to its effects on ion channel pharmacology; again this compound inhibits ROCK2/PRK2 kinases in vitro.

The researchers say that their lead molecule is effective in promoting survival in three different hES cell lines and that their findings emphasize the critical importance of the ROCK2/PRK2 signalling pathway in human embryonic cell survival.

Bart Vanhaesebroeck, Deputy Chair for the BJ ChemBio Knowledge Environment, said: "These findings bring us one step closer to realizing the immense potential of human stem cells for therapeutic benefit."


Story Source:

The above story is based on materials provided by The Biochemical Society. Note: Materials may be edited for content and length.


Journal Reference:

  1. Paul Andrews, Melissa Becroft, Anders Aspegren, Jane Gilmour, Martyn James, Scott McRae, Robert Kime, Robert Allcock, Achamma Abraham, Zhong Jiang, Raimund Strehl, Joanne Mountford, Graeme Milligan, Miles Houslay, David Adams, Julie Frearson. High content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules. Biochemical Journal, 2010; DOI: 10.1042/BJ20101022

Cite This Page:

The Biochemical Society. "Increasing the efficiency of stem cells." ScienceDaily. ScienceDaily, 25 October 2010. <www.sciencedaily.com/releases/2010/10/101025090059.htm>.
The Biochemical Society. (2010, October 25). Increasing the efficiency of stem cells. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2010/10/101025090059.htm
The Biochemical Society. "Increasing the efficiency of stem cells." ScienceDaily. www.sciencedaily.com/releases/2010/10/101025090059.htm (accessed October 20, 2014).

Share This



More Health & Medicine News

Monday, October 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Microneedle Patch Promises Painless Pricks

Microneedle Patch Promises Painless Pricks

Reuters - Innovations Video Online (Oct. 18, 2014) Researchers at The National University of Singapore have invented a new microneedle patch that could offer a faster and less painful delivery of drugs such as insulin and painkillers. Video provided by Reuters
Powered by NewsLook.com
Raw: Nurse Nina Pham Arrives in Maryland

Raw: Nurse Nina Pham Arrives in Maryland

AP (Oct. 17, 2014) The first nurse to be diagnosed with Ebola at a Dallas hospital walked down the stairs of an executive jet into an ambulance at an airport in Frederick, Maryland, on Thursday. Pham will be treated at the National Institutes of Health. (Oct. 16) Video provided by AP
Powered by NewsLook.com
Raw: Cruise Ship Returns to US Over Ebola Fears

Raw: Cruise Ship Returns to US Over Ebola Fears

AP (Oct. 17, 2014) A Caribbean cruise ship carrying a Dallas health care worker who is being monitored for signs of the Ebola virus is heading back to Texas, US, after being refused permission to dock in Cozumel, Mexico. (Oct. 17) Video provided by AP
Powered by NewsLook.com
Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

AFP (Oct. 17, 2014) All four suspected Ebola cases admitted to hospitals in Spain on Thursday have tested negative for the deadly virus in a first round of tests, the government said Friday. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins