Featured Research

from universities, journals, and other organizations

Earliest cardiovascular progenitors that arise during the differentiation of pluripotent stem cells isolated

Date:
March 8, 2011
Source:
Libre de Bruxelles, Université
Summary:
Pluripotent stem cells and induced pluripotent stem cells (iPS) have the capacity to differentiate into any cell type in the body, including cardiac and vascular cells, which give hope that one day, we can use these cells to replace the death or damaged cells in various diseases. The discovery of novel methods allowing the purification of cardiovascular progenitors during embryonic stem cell differentiation is thus essential before these cells could be used in large scale to treat patients suffering from cardiovascular diseases or for drug discovery.

Researchers from the Université libre de Bruxelles(ULB) led by Dr. Cédric Blanpain have isolated the earliest cardiovascular progenitors that arise during the differentiation of pluripotent stem cells.

Related Articles


The heart is composed of different cell types including the contractile muscle cells and the vascular cells. During embryonic development all cardiac cells and certain blood vessels derive from primitive cells known as cardiovascular progenitors.

Pluripotent stem cells and induced pluripotent stem cells (iPS) have the capacity to differentiate into any cell type in the body, including cardiac and vascular cells, which give hope that one day, we can use these cells to replace the death or damaged cells in various diseases. The discovery of novel methods allowing the purification of cardiovascular progenitors during embryonic stem cell differentiation is thus essential before these cells could be used in large scale to treat patients suffering from cardiovascular diseases or for drug discovery.

Researchers led by Dr Cédric Blanpain, FNRS researcher at IRIBHM, Université libre de Bruxelles (ULB), Belgium, studied the mechanisms that govern the specification of cardiovascular progenitors during pluripotent stem cell differentiation. In a new study published in the Journal of Cell Biology, the ULB researchers used genetically engineered embryonic stem cells that become fluorescent when the stem cells become cardiovascular progenitors. By isolating these fluorescent cells, they purified the cardiovascular progenitors and differentiated these cells into beating cardiac cells in vitro and in vivo.

"It was really amazing," commented Dr Antoine Bondue, postdoctoral fellow of the FNRS and first author of this study, "We can now purify the early cardiovascular progenitors and predict in advance which cells will give rise to cardiac cells. It was like dozens of little hearts were beating in the petri dish containing the fluorescent progenitors while no cell was contracting in the dish containing the non fluorescent cells."

Using whole genome analysis of these isolated fluorescent cardiovascular progenitors, the researchers identified novel markers expressed by these cells that allow for their isolation without the need of any other genetic manipulations. This important finding will provide, to the scientific community as well as the pharmaceutical industry, a simple and robust method to isolate cardiovascular progenitors arising from the differentiation of pluripotent stem cells. "This finding will be of great help to upscale the production of cardiovascular cells obtained during pluripotent stem cells differentiation, to better understand what are the conditions needed to amplify cardiovascular progenitors further and how can we push the differentiation of these cells into very specific cardiac or vascular cell types, and hopefully this will accelerate the basic research necessary before the physician will be able to use safely cardiovascular cells to treat patients suffering from cardiovascular diseases, and more importantly to be able to provide to these patients a clear clinical benefit," commented Dr Blanpain.

This work was supported by the Belgian FNRS, a career development award of the Human Frontier Science Program Organization (HFSPO), la Fondation ULB, a starting grant of the European Research Council (ERC) and the EMBO Young Investigator Program.


Story Source:

The above story is based on materials provided by Libre de Bruxelles, Université. Note: Materials may be edited for content and length.


Journal Reference:

  1. A. Bondue, S. Tannler, G. Chiapparo, S. Chabab, M. Ramialison, C. Paulissen, B. Beck, R. Harvey, C. Blanpain. Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation. The Journal of Cell Biology, 2011; 192 (5): 751 DOI: 10.1083/jcb.201007063

Cite This Page:

Libre de Bruxelles, Université. "Earliest cardiovascular progenitors that arise during the differentiation of pluripotent stem cells isolated." ScienceDaily. ScienceDaily, 8 March 2011. <www.sciencedaily.com/releases/2011/03/110308155808.htm>.
Libre de Bruxelles, Université. (2011, March 8). Earliest cardiovascular progenitors that arise during the differentiation of pluripotent stem cells isolated. ScienceDaily. Retrieved April 21, 2015 from www.sciencedaily.com/releases/2011/03/110308155808.htm
Libre de Bruxelles, Université. "Earliest cardiovascular progenitors that arise during the differentiation of pluripotent stem cells isolated." ScienceDaily. www.sciencedaily.com/releases/2011/03/110308155808.htm (accessed April 21, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Tuesday, April 21, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Teva Offers $40 Billion for Mylan

Teva Offers $40 Billion for Mylan

Reuters - Business Video Online (Apr. 21, 2015) — Generic drugmaker Teva Pharmaceutical is offering $82 a share, or $40 billion, for its smaller rival Mylan, in an alternative to Mylan&apos;s deal to buy Perrigo. Bobbi Rebell reports. Video provided by Reuters
Powered by NewsLook.com
Blue Bell Recalls All Products

Blue Bell Recalls All Products

AP (Apr. 21, 2015) — Blue Bell Creameries voluntary recalled for all of its products after two samples of chocolate chip cookie dough ice cream tested positive for listeria, a potentially deadly bacteria. Blue Bell&apos;s President and CEO issued a video statement. (April 21) Video provided by AP
Powered by NewsLook.com
Yemen Doctors at Breaking Point

Yemen Doctors at Breaking Point

Reuters - News Video Online (Apr. 21, 2015) — A Sanaa hospital struggles to cope with the high number of casualties with severe injuries, after an air strike left at least 25 dead and hundreds wounded. Deborah Lutterbeck reports. Video provided by Reuters
Powered by NewsLook.com
'Tutu Tuesdays' Brighten Faces at Kids' Hospital

'Tutu Tuesdays' Brighten Faces at Kids' Hospital

AP (Apr. 21, 2015) — Doctors and nurses have started wearing ballet tutus every Tuesday to cheer up young hospital patients at a Florida hospital. It started with a request made by a nervous patient -- now, almost the entire staff is wearing the tutus. (April 21) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins