June 13, 2011 A team of scientists has developed a way to coax tumor cells in the lab to grow into 3-D spheres. Their discovery takes advantage of an earlier technique of producing spherical cavities in a common polymer and promises more accurate tests of new cancer therapies.
As team leader Michael R. King, Ph.D., of Cornell University explains, "Sometimes engineering research tends to be a case of a hammer looking for a nail. We knew our previous discovery was new and it was cool. And now we know it's useful."
Three years ago, the team -- in collaboration with Lisa DeLouise, Ph.D., MPD, of Rochester, N.Y. -- perfected a low-cost, easy fabrication technique to make spherical cavities in PDMS (polydimethylsiloxane), a widely used silicon organic polymer. More recently, the Cornell team discovered that these cavities could be used as a scaffolding to grow numerous tumor spheroids, which could serve as realistic models for cancer cells. The Cornell team's work appears in the current issue of Biomicrofluidics, a publication of the American Institute of Physics.
The three-dimensional spheroids hold the potential to speed cancer drug discovery by providing a realistic and easily accessible substrate on which to test drugs. Their 3-D nature is an asset because in the body, tumor cells grow in 3-D -- yet most laboratory studies of cancer have been done in 2-D, with a single layer of cancer cells grown on the bottom of a petri dish. Too often a promising 2-D drug candidate fails when it enters the 3-D stage of animal testing. The new 3-D tumor spheroids may help eliminate that problem. They also offer a realistic tumor oxygen environment that cues the blood vessel growth that nourishes tumors -- an appealing target for anti-cancer drug design.
"Basically, any laboratory that works with cells could adopt our new spherical microcavity system to do their own 3-D experiments or drug screening on hundreds or even thousands of little tumor spheroids," said King.
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- Sivaprakash Agastin, Ut-Binh T. Giang, Yue Geng, Lisa A. DeLouise, Michael R. King. Continuously perfused microbubble array for 3D tumor spheroid model. Biomicrofluidics, 2011; 5 (2): 024110 DOI: 10.1063/1.3596530
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