Featured Research

from universities, journals, and other organizations

Immune cells use tethered slings to avoid being swept away

Date:
December 17, 2012
Source:
American Society for Cell Biology
Summary:
Neutrophils, critical components of the immune system's response to bacteria and other pathogens, throw out tube-like tethers that act as anchor points, controlling their speed as they roll along the walls of blood vessels during extremely fast blood flow en route to an infection site.

Neutrophils, critical components of the immune system's response to bacteria and other pathogens, throw out tube-like tethers that act as anchor points, controlling their speed as they roll along the walls of blood vessels during extremely fast blood flow en route to an infection site, according to research presented on Dec. 17 at the American Society for Cell Biology Annual Meeting in San Francisco.

To attack a bacterial infection in tissue, neutrophils have to leave the blood stream and approach the infection site through tiny venules that are part of the microcirculation system, according to Prithu Sundd, PhD, who is in the laboratory of Klaus Ley, PhD, at the La Jolla Institute for Allergy and Immunology, La Jolla, CA.

Extensions of the cell membrane, the slings turned out to be vital aids in the navigation because if neutrophils lose control while attempting to enter infected tissue, they can be swept away in the blood flow, which could delay the immune defense mechanism. Flow in these narrow venules is measured as wall shear stress. A shear stress exceeding 2 dyn/cm2 can sweep away other leukocytes, but neutrophils have a special ability to move under control at shear stress 10 times higher.

Shear-resistance in neutrophils was known to be aided by cell flattening and by these mysterious membrane extensions but the details were poorly understood. To determine the exact mechanism behind neutrophils' rolling, Dr. Sundd working with physicist Alex Groisman, PhD, of the University of California, San Diego, to shoot a high-speed video, using total internal reflection fluorescence microscopy to track labeled neutrophils from mouse bone marrow rolling along an artificial venule, all driven by a microfluidic device at a shear stress of 6 to 10 dyn/cm2.

In the 15-second video, the red-dyed neutrophil used its long membrane tether like a sling to anchor itself without being swept away by the high shear force of blood. Instead of a single anchor point, the sling tether is coated by patches of cell adhesion molecules that latched onto the passage walls but peeled loose, patch by patch, as the neutrophil gently rolled forward. At the tether's end, the neutrophil swung it ahead like a lasso to gain new leverage.

The researchers say their dramatic video underscores the complexity of the body's immune system. The slings are not only unique structures, says Dr. Sundd, but may help explain how rolling neutrophils are able to present their antigen-sensing ligands at the blood vessel wall before entering the site of infection.

This study was supported by the NCRP-Scientist Development Grant (11SDG7340005) from American Heart Association (PS), WSA postdoctoral fellowship (10POST4160142-01) from American Heart Association (EKK), and National Institutes of Health (EB02185) (KL).


Story Source:

The above story is based on materials provided by American Society for Cell Biology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Prithu Sundd, Edgar Gutierrez, Ekaterina K. Koltsova, Yoshihiro Kuwano, Satoru Fukuda, Maria K. Pospieszalska, Alex Groisman, Klaus Ley. ‘Slings’ enable neutrophil rolling at high shear. Nature, 2012; 488 (7411): 399 DOI: 10.1038/nature11248

Cite This Page:

American Society for Cell Biology. "Immune cells use tethered slings to avoid being swept away." ScienceDaily. ScienceDaily, 17 December 2012. <www.sciencedaily.com/releases/2012/12/121217140539.htm>.
American Society for Cell Biology. (2012, December 17). Immune cells use tethered slings to avoid being swept away. ScienceDaily. Retrieved September 1, 2014 from www.sciencedaily.com/releases/2012/12/121217140539.htm
American Society for Cell Biology. "Immune cells use tethered slings to avoid being swept away." ScienceDaily. www.sciencedaily.com/releases/2012/12/121217140539.htm (accessed September 1, 2014).

Share This




More Health & Medicine News

Monday, September 1, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Get on Your Bike! London Cycling Popularity Soars Despite Danger

Get on Your Bike! London Cycling Popularity Soars Despite Danger

AFP (Sep. 1, 2014) Wedged between buses, lorries and cars, cycling in London isn't for the faint hearted. Nevertheless the number of people choosing to bike in the British capital has doubled over the past 15 years. Duration: 02:27 Video provided by AFP
Powered by NewsLook.com
Can You Train Your Brain To Eat Healthy?

Can You Train Your Brain To Eat Healthy?

Newsy (Sep. 1, 2014) New research says if you condition yourself to eat healthy foods, eventually you'll crave them instead of junk food. Video provided by Newsy
Powered by NewsLook.com
We've Got Mites Living In Our Faces And So Do You

We've Got Mites Living In Our Faces And So Do You

Newsy (Aug. 30, 2014) A new study suggests 100 percent of adult humans (those over 18 years of age) have Demodex mites living in their faces. Video provided by Newsy
Powered by NewsLook.com
Liberia Continues Fight Against Ebola

Liberia Continues Fight Against Ebola

AFP (Aug. 30, 2014) Authorities in Liberia try to stem the spread of the Ebola epidemic by raising awareness and setting up sanitation units for people to wash their hands. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins