Featured Research

from universities, journals, and other organizations

3-D stem cell culture technique developed to better understand Alzheimer's disease

Date:
April 2, 2013
Source:
New York Stem Cell Foundation
Summary:
A team of researchers has developed a technique to produce three-dimensional cultures of induced pluripotent stem cells called embryoid bodies, amenable to live cell imaging and to electrical activity measurement.

A team of researchers at The New York Stem Cell Foundation Research Institute led by Scott Noggle, PhD, Director of the NYSCF Laboratory and the NYSCF -- Charles Evans Senior Research Fellow for Alzheimer's Disease, and Michael W. Nestor, PhD, a NYSCF Postdoctoral Research Fellow, has developed a technique to produce three-dimensional cultures of induced pluripotent stem (iPS) cells called embryoid bodies, amenable to live cell imaging and to electrical activity measurement.

As reported in their Stem Cell Research study, these cell aggregates enable scientists to both model and to study diseases such as Alzheimer's and Parkinson's disease.

The NYSCF Alzheimer's disease research team aims to better understand and to find treatments to this disease through stem cell research. For such disorders in which neurons misfire or degenerate, the NYSCF team creates "disease in a dish" models by reprogramming patients' skin and or blood samples into induced pluripotent stem (iPS) cells that can become neurons and the other brain cells affected in the diseases.

The cells in our body form three-dimensional networks, essential to tissue function and overall health; however, previous techniques to form complex brain tissue resulted in structures that, while similar in form to naturally occurring neurons, undermined imaging or electrical recording attempts.

In the current study, Noggle and Nestor with NYSCF scientists specially adapted two-dimensional culture methods to grow three-dimensional neuron structures from iPS cells. The resultant neurons were "thinned-out," enabling calcium-imaging studies, which measure the electrical activity of cells like neurons.

"Combining the advantages of iPS cells grown in a 3D environment with those of a 2D system, our technique produces cells that can be used to observe electrical activity of putative networks of biologically active neurons, while simultaneously imaging them," said Nestor. "This is key to modeling and studying neurodegenerative diseases."

Neural networks, thought to underlie learning and memory, become disrupted in Alzheimer's disease. By generating aggregates from iPS cells and comparing these to an actual patient's brain tissue, scientists may uncover how disease interferes with these cell-to-cell interactions and understand how to intervene to slow or stop Alzheimer's disease.

"This critical new tool developed by our Alzheimer's team will accelerate Alzheimer's research, enabling more accurate manipulation of cells to find a cure to this disease," said Susan L. Solomon, CEO of NYSCF.


Story Source:

The above story is based on materials provided by New York Stem Cell Foundation. Note: Materials may be edited for content and length.


Journal Reference:

  1. Michael W. Nestor, Daniel Paull, Samson Jacob, Andrew A. Sproul, Abdulaziz Alsaffar, Brian A. Campos, Scott A. Noggle. Differentiation of serum-free embryoid bodies from human induced pluripotent stem cells into networks. Stem Cell Research, 2013; 10 (3): 454 DOI: 10.1016/j.scr.2013.02.001

Cite This Page:

New York Stem Cell Foundation. "3-D stem cell culture technique developed to better understand Alzheimer's disease." ScienceDaily. ScienceDaily, 2 April 2013. <www.sciencedaily.com/releases/2013/04/130402091656.htm>.
New York Stem Cell Foundation. (2013, April 2). 3-D stem cell culture technique developed to better understand Alzheimer's disease. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2013/04/130402091656.htm
New York Stem Cell Foundation. "3-D stem cell culture technique developed to better understand Alzheimer's disease." ScienceDaily. www.sciencedaily.com/releases/2013/04/130402091656.htm (accessed July 31, 2014).

Share This




More Health & Medicine News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

House Republicans Vote to Sue Obama Over Healthcare Law

House Republicans Vote to Sue Obama Over Healthcare Law

Reuters - US Online Video (July 31, 2014) The Republican-led House of Representatives votes to sue President Obama, accusing him of overstepping his executive authority in making changes to the Affordable Care Act. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com
Despite Health Questions, E-Cigs Are Beneficial: Study

Despite Health Questions, E-Cigs Are Beneficial: Study

Newsy (July 31, 2014) Citing 81 previous studies, new research out of London suggests the benefits of smoking e-cigarettes instead of regular ones outweighs the risks. Video provided by Newsy
Powered by NewsLook.com
Dangerous Bacteria Kills One in Florida

Dangerous Bacteria Kills One in Florida

AP (July 31, 2014) Sarasota County, Florida health officials have issued a warning against eating raw oysters and exposing open wounds to coastal and inland waters after a dangerous bacteria killed one person and made another sick. (July 31) Video provided by AP
Powered by NewsLook.com
Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



    Save/Print:
    Share:

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile: iPhone Android Web
    Follow: Facebook Twitter Google+
    Subscribe: RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins