Featured Research

from universities, journals, and other organizations

Secrets of bacterial slime revealed

Date:
April 12, 2013
Source:
Newcastle University
Summary:
Scientists have revealed the mechanism that causes a slime to form, making bacteria hard to shift and resistant to antibiotics.

Structure of SinR bound to DNA. SinR inhibits the formation of the molecular glue that holds a biofilm together by binding to specific targets on DNA. These targets are spaced so that the DNA forms a loop around the protein.
Credit: Image courtesy of Newcastle University

Newcastle University scientists have revealed the mechanism that causes a slime to form, making bacteria hard to shift and resistant to antibiotics.

When under threat, some bacteria can shield themselves in a slimy protective layer, known as a biofilm. It is made up of communities of bacteria held together to protect themselves from attack.

Biofilms cause dental plaque and sinusitis; in healthcare, biofilms can lead to life threatening and difficult to treat infections, particularly on medical implants such as catheters, heart valves, artificial hips and even breast implants. They also they coat the outside of ships and boats polluting the water.

Publishing in the Journal of Biological Chemistry, the team reveal how a molecular switch regulates biofilm formation. This new understanding could help identify a new target for antibiotics and prevent other biofilms from forming.

In order to thwart them from causing disease and biopollution, a Newcastle University team have been studying at the molecular level how bacteria form biofilms in the first instance.

They reveal how the master regulator of biofilm formation, a protein called SinR, acts in the model bacterium, Bacillus subtilis.

Richard Lewis, Professor of Structural Biology in the Institute for Cell and Molecular Biosciences who led the research said: "SinR is a bit like a rocker switch, a domestic light switch for instance. In the "down" position, when SinR is bound to DNA, the proteins required to make a biofilm are turned off and the bacteria are free to move. In the "up" position, SinR is no longer bound to DNA and instead interacts with other proteins, and the biofilms genes are turned on."

SinR is a DNA-binding protein that acts to inhibit the expression of proteins required for the synthesis of the molecular glue that holds the biofilm together. The ability of SinR to bind to DNA is carefully controlled by a network of interactions with three other proteins. By the application of X-ray crystallography, the team have determined precisely how SinR interacts with very specific feature of its DNA target.

By understanding how the proteins interact with each other, and with DNA, scientists can look to develop molecules that interfere with these essential processes as a means to stop biofilms from forming.


Story Source:

The above story is based on materials provided by Newcastle University. Note: Materials may be edited for content and length.


Journal Reference:

  1. J. A. Newman, C. Rodrigues, R. J. Lewis. Molecular Basis of the Activity of SinR Protein, the Master Regulator of Biofilm Formation in Bacillus subtilis. Journal of Biological Chemistry, 2013; 288 (15): 10766 DOI: 10.1074/jbc.M113.455592

Cite This Page:

Newcastle University. "Secrets of bacterial slime revealed." ScienceDaily. ScienceDaily, 12 April 2013. <www.sciencedaily.com/releases/2013/04/130412132413.htm>.
Newcastle University. (2013, April 12). Secrets of bacterial slime revealed. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2013/04/130412132413.htm
Newcastle University. "Secrets of bacterial slime revealed." ScienceDaily. www.sciencedaily.com/releases/2013/04/130412132413.htm (accessed July 24, 2014).

Share This




More Plants & Animals News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Dogs Appear To Become Jealous Of Owners' Attention

Dogs Appear To Become Jealous Of Owners' Attention

Newsy (July 23, 2014) A U.C. San Diego researcher says jealousy isn't just a human trait, and dogs aren't the best at sharing the attention of humans with other dogs. Video provided by Newsy
Powered by NewsLook.com
Professor Creates Site Revealing Where People's Cats Live

Professor Creates Site Revealing Where People's Cats Live

Newsy (July 23, 2014) ​It's called I Know Where Your Cat Lives, and you can keep hitting the "Random Cat" button to find more real cats all over the world. Video provided by Newsy
Powered by NewsLook.com
Stone Fruit Listeria Scare Causes Sweeping Recall

Stone Fruit Listeria Scare Causes Sweeping Recall

Newsy (July 22, 2014) The Wawona Packing Company has issued a voluntary recall on the stone fruit it distributes due to a possible Listeria outbreak. Video provided by Newsy
Powered by NewsLook.com
Michigan Plant's Goal: Flower and Die

Michigan Plant's Goal: Flower and Die

AP (July 22, 2014) An 80-year-old agave plant, which is blooming for the first and only time at a University of Michigan conservatory, will die when it's done (July 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins