Featured Research

from universities, journals, and other organizations

Scientists make your stomach turn bright green if you have an ulcer

Date:
January 8, 2014
Source:
University of Southern Denmark
Summary:
Doctors may soon be able to diagnose stomach ulcers without taking tissue samples from the stomach. Researchers now report to have developed a new, safer and noninvasive diagnostic technique for ulcers. The trick is to make the ulcer-causing bacteria in the gut light up in fluorescent green.

This is a photo of Silvia Fontenente.
Credit: Birgitte Svennevig/SDU

Doctors may soon be able to diagnose stomach ulcers without taking tissue samples from the stomach. Researchers from the University of Southern Denmark now report to have developed a new, safer and noninvasive diagnostic technique for ulcers. The trick is to make the ulcer-causing bacteria in the gut light up in fluorescent green.

Each year, many patients are examined for ulcers, and this is often done by retrieving a tissue sample from the stomach. This requires that the doctor sends an instrument down into the patient´s stomach, and the patient must wait for the tissue sample to be analyzed before the doctor can give information about a possible ulcer.

Now researchers from University of Southern Denmark are developing a diagnostic technique that makes it possible to detect the ulcer instantly. It shortens the patient's unease and it improves the doctor's opportunity for early diagnosis.

"Early diagnosis does not only prevent ulcers from developing, it can also prevent the development of cancer," says Ph.D. student Silvia Fontenete from the Nucleic Acid Center at the University of Southern Denmark/Laboratory for Process Engineering, Environment, Biotechnology and Energy, University of Porto in Portugal. She is the lead author of an article on the research results in journals PLOS ONE.

Ulcers are often caused by the bacteria Helicobacter pylori, which produces ulcers in the stomach or duodenum. Usually the doctor retrieves a tissue sample from the stomach and has it analyzed. You can also take a breath test, where molecules in the patient's breath are analyzed, but this technique is not always reliable, says Silvia Fontenete.

Scientists have for some time been able to make tissue samples from the stomach glow fluorescent green if the tissue is infected with H. pylori bacteria. For this the scientist will need to retrieve a piece of the stomach for analysis in the laboratory.

The researchers' new approach is to make the stomach glow bright fluorescent green WITHOUT taking tissue samples from it.

"Our laboratory experiments suggest that one day it will be possible for doctors to send some specially designed molecules down in the stomach, where they will make H. pylori glow brightly green," explains Silvia Fontenete.

To see the green light the doctor will send a small micro-camera into the stomach.

The scientists report to have made H. pylori glow green in artificial tissue in the laboratory -- this was tissue that mimics the lining of the human stomach.

"We believe that the same can happen in a real human stomach," says Silvia Fontenete.

To achieve their results, the researchers had to overcome two major challenges: The first challenge was to create the special molecules that can both detect H. pylori bacteria and function at approx. 37 degrees Celsius, which is the temperature of the human stomach. The second challenge was that the molecule should be able to function in the extremely acidic environment of the stomach.

Both challenges were solved by working with the so-called Locked Nucleic Acid (LNA), some special synthetic molecules developed by Silvia Fontenente's research director, Professor Jesper Wengel at University of Southern Denmark. LNA are artificial RNA-like molecules which can bind to microRNA molecules. They are extremely stable and can operate at lower temperatures and in more acidic environments than other molecules.

"We have been lucky: The first molecules I created turned out to do the job right away. I could have been more unfortunate, and it would have taken several years," says Silvia Fontenete.


Story Source:

The above story is based on materials provided by University of Southern Denmark. The original article was written by Birgitte Svennevig. Note: Materials may be edited for content and length.


Journal Reference:

  1. Sílvia Fontenete, Nuno Guimarães, Marina Leite, Céu Figueiredo, Jesper Wengel, Nuno Filipe Azevedo. Hybridization-Based Detection of Helicobacter pylori at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes. PLoS ONE, 2013; 8 (11): e81230 DOI: 10.1371/journal.pone.0081230

Cite This Page:

University of Southern Denmark. "Scientists make your stomach turn bright green if you have an ulcer." ScienceDaily. ScienceDaily, 8 January 2014. <www.sciencedaily.com/releases/2014/01/140108112656.htm>.
University of Southern Denmark. (2014, January 8). Scientists make your stomach turn bright green if you have an ulcer. ScienceDaily. Retrieved April 19, 2014 from www.sciencedaily.com/releases/2014/01/140108112656.htm
University of Southern Denmark. "Scientists make your stomach turn bright green if you have an ulcer." ScienceDaily. www.sciencedaily.com/releases/2014/01/140108112656.htm (accessed April 19, 2014).

Share This



More Health & Medicine News

Saturday, April 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

'Holy Grail' Of Weight Loss? New Find Could Be It

'Holy Grail' Of Weight Loss? New Find Could Be It

Newsy (Apr. 18, 2014) — In a potential breakthrough for future obesity treatments, scientists have used MRI scans to pinpoint brown fat in a living adult for the first time. Video provided by Newsy
Powered by NewsLook.com
Little Progress Made In Fighting Food Poisoning, CDC Says

Little Progress Made In Fighting Food Poisoning, CDC Says

Newsy (Apr. 18, 2014) — A new report shows rates of two foodborne infections increased in the U.S. in recent years, while salmonella actually dropped 9 percent. Video provided by Newsy
Powered by NewsLook.com
Scientists Create Stem Cells From Adult Skin Cells

Scientists Create Stem Cells From Adult Skin Cells

Newsy (Apr. 17, 2014) — The breakthrough could mean a cure for some serious diseases and even the possibility of human cloning, but it's all still a way off. Video provided by Newsy
Powered by NewsLook.com
Obama: 8 Million Healthcare Signups

Obama: 8 Million Healthcare Signups

AP (Apr. 17, 2014) — President Barack Obama gave a briefing Thursday announcing 8 million people have signed up under the Affordable Care Act. He blasted continued Republican efforts to repeal the law. (April 17) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins