Featured Research

from universities, journals, and other organizations

Ending recurrent urinary tract infections: Study with mice gives hope

Date:
March 25, 2014
Source:
University of Utah Health Sciences
Summary:
FDA-approved compound helps rid bladder of 'reservoir populations' of bacteria that cause recurrent urinary tract infections, a new mouse study has demonstrated. "Antibiotics don't do a good job of getting rid of reservoir populations," researchers explain. "But when augmented with chitosan, there was a significant reduction in the level of bacteria in mouse bladders."

Millions of people worldwide -- mostly women -- suffer from recurrent urinary tract infections (UTIs) that seriously degrade their health and quality of life. Antibiotics treat individual infections, but preventing recurrent ones largely has been unattainable because of the way bacteria lodge in the inner layers of the bladder and quietly hide from drugs that can kill them.

In new studies with mice, however, researchers led by University of Utah microbiologists have shown that when chitosan, an FDA-approved compound for pharmaceutical, agricultural and other uses, is given in conjunction with antibiotics, it assists the bladder in ridding itself of "reservoir" populations of bacteria that live in its deeper layers and potentially cause recurrent infections. If the effectiveness of chitosan (ky-ta-san) against reservoir populations of bacteria could be confirmed in people, the compound might one day serve to augment antibiotic treatments of recurrent UTIs, according to Matthew Blango, Ph.D., a U of U postdoctoral researcher and first author on the study that appeared Tuesday, March 25, 2014, online in the journal PLOS ONE.

"Antibiotics don't do a good job of getting rid of reservoir populations," Blango says. "But when augmented with chitosan, there was a significant reduction in the level of bacteria in mouse bladders."

Matthew Mulvey, Ph.D., U of U professor of pathology senior author on the study. Elizabeth M. Ott, Ph.D., also a U postdoctoral fellow, is a co-author along with two researchers from the University of Ljubljana, Slovenia.

UTIs are responsible for nearly 10 million health care office visits, 1.5 million hospitalizations and $1 billion in costs annually in the United States, according to the National Kidney Foundation. The infections are more common in women, with one in five getting at least one UTI during her lifetime. Unfortunately, after the first infection, some people will get a second, third and fourth or more UTIs, with each successive infection making them more susceptible to recurrent episodes.

The urinary tract comprises the kidneys, bladder, uretha and ureters. UTIs are caused primarily by bacteria known as uropathogenic Escherichia coli (UPEC), which originate in the bowel but move into the urinary tract. UPEC often are susceptible to antibiotics, but they also have the ability to pass through the surface lining in the bladder and permeate into deeper layers of cells and tissue where they lie dormant and invulnerable to drugs. Periodically, though, UPEC bacteria replicate in the tissues, which may cause recurrent UTI flare-ups in people.

To destroy reservoir populations of UPEC, Blango, Mulvey and their colleagues infused mouse bladders with chitosan for 20 minutes, during which time the compound worked to exfoliate large cells lining the surface of the bladders. The exfoliation of cells did not harm the deeper layers of the mouse bladders but caused dormant reservoirs of UPEC to move toward the surface of the bladders and divide and replicate, making them susceptible to antibiotics.

The researchers followed up the chitosan by giving the mice a one-week course of fluoroquinolones, an antibiotic class commonly used to treat UTIs. A week later, when they checked the level of UPEC in the mouse bladders, Blango and his colleagues found the reservoir populations of the bacteria for the most part were gone.

"Effectively, there were no bacteria in the bladder," Blango says.

The mouse bladders also regenerated the surface lining of exfoliated cells in about a week.

The results in mice don't mean that people with recurrent UTIs can or should start taking chitosan with antibiotics. Large-scale trials with people would be needed to verify the efficacy of the compound in humans. Blango's and Mulvey's colleagues in Slovania are looking into that prospect, while the U of U researchers are interested in refining and improving the results in mice.


Story Source:

The above story is based on materials provided by University of Utah Health Sciences. Note: Materials may be edited for content and length.


Journal Reference:

  1. Matthew G. Blango, Elizabeth M. Ott, Andreja Erman, Peter Veranic, Matthew A. Mulvey. Forced Resurgence and Targeting of Intracellular Uropathogenic Escherichia coli Reservoirs. PLoS ONE, 2014; 9 (3): e93327 DOI: 10.1371/journal.pone.0093327

Cite This Page:

University of Utah Health Sciences. "Ending recurrent urinary tract infections: Study with mice gives hope." ScienceDaily. ScienceDaily, 25 March 2014. <www.sciencedaily.com/releases/2014/03/140325190821.htm>.
University of Utah Health Sciences. (2014, March 25). Ending recurrent urinary tract infections: Study with mice gives hope. ScienceDaily. Retrieved October 21, 2014 from www.sciencedaily.com/releases/2014/03/140325190821.htm
University of Utah Health Sciences. "Ending recurrent urinary tract infections: Study with mice gives hope." ScienceDaily. www.sciencedaily.com/releases/2014/03/140325190821.htm (accessed October 21, 2014).

Share This



More Health & Medicine News

Tuesday, October 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

How Nigeria Beat Its Ebola Outbreak

How Nigeria Beat Its Ebola Outbreak

Newsy (Oct. 20, 2014) The World Health Organization has declared Nigeria free of Ebola. Health experts credit a bit of luck and the government's initial response. Video provided by Newsy
Powered by NewsLook.com
Another Study Suggests Viagra Is Good For The Heart

Another Study Suggests Viagra Is Good For The Heart

Newsy (Oct. 20, 2014) An ingredient in erectile-dysfunction medications such as Viagra could improve heart function. Perhaps not surprising, given Viagra's history. Video provided by Newsy
Powered by NewsLook.com
Ebola Worries End for Dozens on U.S. Watch Lists

Ebola Worries End for Dozens on U.S. Watch Lists

Reuters - US Online Video (Oct. 20, 2014) Forty-three people who had contact with Thomas Eric Duncan, the first person diagnosed with Ebola in the U.S., were cleared overnight of twice-daily monitoring after 21 days of showing no symptoms. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
CDC Calls for New Ebola Safety Guidelines

CDC Calls for New Ebola Safety Guidelines

AP (Oct. 20, 2014) Centers for Disease Control and Prevention Director Dr. Tom Frieden laid out new guidelines for health care workers when dealing with the deadly Ebola virus including new precautions when taking off personal protective equipment. (Oct. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins