Featured Research

from universities, journals, and other organizations

Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme

Date:
December 23, 1997
Source:
Scripps Research Institute
Summary:
For the first time, scientists at The Scripps Research Institute (TSRI) have developed a catalytic antibody with an efficiency and mechanism equal to that of a natural enzyme essential to life.

For the first time, scientists at The Scripps Research Institute (TSRI) have developed a catalytic antibody with an efficiency and mechanism equal to that of a natural enzyme essential to life. According to Richard Lerner, M.D., TSRI President and the study's author, "We have simulated an important enzyme via an antibody, while broadening its specificity. In addition, this will be the first commercially available catalytic antibody." The scientists believe that it will have numerous applications in industrial synthesis, including the synthesis of some of the most important anticancer compounds.

Related Articles


The work, "Immune Versus Natural Selection: Antibody Aldolases with Enzymic Rates but Broader Scope" appears in the Dec. 18 issue of Science. Other authors include Carlos F. Barbas, III, Andreas Heine, Guofu Zhong, Torsten Hoffmann, Svetlana Gramatikova, Robert Bjornestedt, Benjamin List, James Anderson, Enrico A. Stura, and Ian A. Wilson. The researchers are members of The Skaggs Institute for Chemical Biology and the Department of Molecular Biology at TSRI.

The scientists compared aldolases that use the same chemical mechanism but differ in their origin. One is a naturally-evolved enzyme and the other, a catalytic antibody developed by reactive immunization. The work solves the dilemma of whether the immune system is capable of creating efficient catalysts by altering its selection criteria from simple binding to function.

While antibodies generally bind non-covalently with their substrates, the technique of reactive immunization enables catalytic antibodies to react with antigens, allowing the catalysis of chemical reactions previously thought to be impossible. In this case, the antibodies catalyze the aldol reaction, an important carbon-carbon bond-forming reaction and one of the most widely used in making pharmaceuticals, and diagnostic and imaging materials.

Because the natural catalysts are too highly restricted in the substrates they use to be of general use to chemists, the scientists also aimed to generate antibodies that are capable of catalyzing reactions with a greater range of substrates than the enzymes that exist in nature.

By moving from the creation of antibodies using the principle of transition-state stabilization to reactive immunization, the scientists have created an antibody that behaves in similar manner to the natural enzyme with regard to its reaction chemistry. In so doing, they have programmed a set of binding pockets to interact with substrates in much the same way as the natural catalyst. According to Lerner, this directly addresses the issue of whether proteins can be made with comparable catalytic efficiency as enzymes when each uses a similar mechanism. He commented, "While we would not suggest that catalytic antibodies will ultimately prove to be as efficient as all enzymes, this work demonstrates that we can develop an antibody whose efficiency can approximate that of a natural enzyme whose function is essential to all life."

Further, this study provides insights into the evolution of metabolic enzymes that relate to theories of the origins of life.

Funding for the study was provided by the National Institutes of Health and The Skaggs Institute for Chemical Biology.


Story Source:

The above story is based on materials provided by Scripps Research Institute. Note: Materials may be edited for content and length.


Cite This Page:

Scripps Research Institute. "Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme." ScienceDaily. ScienceDaily, 23 December 1997. <www.sciencedaily.com/releases/1997/12/971223070617.htm>.
Scripps Research Institute. (1997, December 23). Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme. ScienceDaily. Retrieved November 28, 2014 from www.sciencedaily.com/releases/1997/12/971223070617.htm
Scripps Research Institute. "Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme." ScienceDaily. www.sciencedaily.com/releases/1997/12/971223070617.htm (accessed November 28, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Friday, November 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Rural India's Low-Cost Sanitary Pad Revolution

Rural India's Low-Cost Sanitary Pad Revolution

AFP (Nov. 28, 2014) — One man hopes his invention -– a machine that produces cheap sanitary pads –- will help empower Indian women. Duration: 01:51 Video provided by AFP
Powered by NewsLook.com
Research on Bats Could Help Develop Drugs Against Ebola

Research on Bats Could Help Develop Drugs Against Ebola

AFP (Nov. 28, 2014) — In Africa's only biosafety level 4 laboratory, scientists have been carrying out experiments on bats to understand how virus like Ebola are being transmitted, and how some of them resist to it. Duration: 01:18 Video provided by AFP
Powered by NewsLook.com
WHO Says Male Ebola Survivors Should Abstain From Sex

WHO Says Male Ebola Survivors Should Abstain From Sex

Newsy (Nov. 28, 2014) — WHO cites four studies that say Ebola can still be detected in semen up to 82 days after the onset of symptoms. Video provided by Newsy
Powered by NewsLook.com
Ebola Leaves Orphans Alone in Sierra Leone

Ebola Leaves Orphans Alone in Sierra Leone

AFP (Nov. 27, 2014) — The Ebola epidemic sweeping Sierra Leone is having a profound effect on the country's children, many of whom have been left without any family members to support them. Duration: 01:02 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins