Featured Research

from universities, journals, and other organizations

Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme

Date:
December 23, 1997
Source:
Scripps Research Institute
Summary:
For the first time, scientists at The Scripps Research Institute (TSRI) have developed a catalytic antibody with an efficiency and mechanism equal to that of a natural enzyme essential to life.

For the first time, scientists at The Scripps Research Institute (TSRI) have developed a catalytic antibody with an efficiency and mechanism equal to that of a natural enzyme essential to life. According to Richard Lerner, M.D., TSRI President and the study's author, "We have simulated an important enzyme via an antibody, while broadening its specificity. In addition, this will be the first commercially available catalytic antibody." The scientists believe that it will have numerous applications in industrial synthesis, including the synthesis of some of the most important anticancer compounds.

The work, "Immune Versus Natural Selection: Antibody Aldolases with Enzymic Rates but Broader Scope" appears in the Dec. 18 issue of Science. Other authors include Carlos F. Barbas, III, Andreas Heine, Guofu Zhong, Torsten Hoffmann, Svetlana Gramatikova, Robert Bjornestedt, Benjamin List, James Anderson, Enrico A. Stura, and Ian A. Wilson. The researchers are members of The Skaggs Institute for Chemical Biology and the Department of Molecular Biology at TSRI.

The scientists compared aldolases that use the same chemical mechanism but differ in their origin. One is a naturally-evolved enzyme and the other, a catalytic antibody developed by reactive immunization. The work solves the dilemma of whether the immune system is capable of creating efficient catalysts by altering its selection criteria from simple binding to function.

While antibodies generally bind non-covalently with their substrates, the technique of reactive immunization enables catalytic antibodies to react with antigens, allowing the catalysis of chemical reactions previously thought to be impossible. In this case, the antibodies catalyze the aldol reaction, an important carbon-carbon bond-forming reaction and one of the most widely used in making pharmaceuticals, and diagnostic and imaging materials.

Because the natural catalysts are too highly restricted in the substrates they use to be of general use to chemists, the scientists also aimed to generate antibodies that are capable of catalyzing reactions with a greater range of substrates than the enzymes that exist in nature.

By moving from the creation of antibodies using the principle of transition-state stabilization to reactive immunization, the scientists have created an antibody that behaves in similar manner to the natural enzyme with regard to its reaction chemistry. In so doing, they have programmed a set of binding pockets to interact with substrates in much the same way as the natural catalyst. According to Lerner, this directly addresses the issue of whether proteins can be made with comparable catalytic efficiency as enzymes when each uses a similar mechanism. He commented, "While we would not suggest that catalytic antibodies will ultimately prove to be as efficient as all enzymes, this work demonstrates that we can develop an antibody whose efficiency can approximate that of a natural enzyme whose function is essential to all life."

Further, this study provides insights into the evolution of metabolic enzymes that relate to theories of the origins of life.

Funding for the study was provided by the National Institutes of Health and The Skaggs Institute for Chemical Biology.


Story Source:

The above story is based on materials provided by Scripps Research Institute. Note: Materials may be edited for content and length.


Cite This Page:

Scripps Research Institute. "Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme." ScienceDaily. ScienceDaily, 23 December 1997. <www.sciencedaily.com/releases/1997/12/971223070617.htm>.
Scripps Research Institute. (1997, December 23). Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme. ScienceDaily. Retrieved August 20, 2014 from www.sciencedaily.com/releases/1997/12/971223070617.htm
Scripps Research Institute. "Scientists Develop Catalytic Antibody With Comparable Efficiency And Of Broader Use Than Natural Enzyme." ScienceDaily. www.sciencedaily.com/releases/1997/12/971223070617.htm (accessed August 20, 2014).

Share This




More Health & Medicine News

Wednesday, August 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Possible Ebola Patient in Isolation at California Hospital

Possible Ebola Patient in Isolation at California Hospital

Reuters - US Online Video (Aug. 20, 2014) — A patient who may have been exposed to the Ebola virus is in isolation at the Kaiser Permanente South Sacramento Medical Center. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Raw: World's Oldest Man Lives in Japan

Raw: World's Oldest Man Lives in Japan

AP (Aug. 20, 2014) — A 111-year-old Japanese was certified as the world's oldest man by Guinness World Records on Wednesday. Sakari Momoi, a native of Fukushima in northern Japan, was given a certificate at a hospital in Tokyo. (Aug. 20) Video provided by AP
Powered by NewsLook.com
Do More Wedding Guests Make A Happier Marriage?

Do More Wedding Guests Make A Happier Marriage?

Newsy (Aug. 20, 2014) — A new study found couples who had at least 150 guests at their weddings were more likely to report being happy in their marriages. Video provided by Newsy
Powered by NewsLook.com
Ebola-Hit Sierra Leone's Freetown a City on Edge

Ebola-Hit Sierra Leone's Freetown a City on Edge

AFP (Aug. 19, 2014) — Residents of Sierra Leone's capital voice their fears as the Ebola virus sweeps through west Africa. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins