Featured Research

from universities, journals, and other organizations

Scientists Fix Mutated Human Tumor-Stopping Gene In Yeast Cells

Date:
April 1, 1998
Source:
Johns Hopkins Medical Institutions
Summary:
Johns Hopkins researchers have found a way to fix "broken" copies of p53, a gene that normally stops the development of pre-cancerous and cancerous cells.

Johns Hopkins researchers have found a way to fix "broken" copies of p53, a gene that normally stops the development of pre-cancerous and cancerous cells.

Related Articles


Using yeast cells genetically engineered to include p53 with mutations common to human cancers, Hopkins scientists identified spots in the gene where a second mutation could restore much of its function.

"If we can learn to fix the protein from this gene in human tumors, it should make the tumor cells much more treatable," says Jef Boeke, Ph.D., professor of molecular biology and genetics.

Boeke says the yeast cells he used in the study could provide a rapid, inexpensive model for testing drug treatments designed to restore p53.

With funding from the National Institutes of Health and the W.W. Smith Charitable Trust, Boeke's group, led by Rainer K. Brachmann, M.D., transplanted copies of human p53 with common "point" mutations--changes of one character in the genetic code--into yeast cells.

The yeast cells with p53 are set up so that when p53 works, the cells grow.

With the mutated p53 in place, the yeast cells did not grow. But when Boeke's team caused a small percentage to mutate, a few cells (less than one in a million) started to grow again. Researchers isolated these cells and identified second small mutations that restored p53 function.

"Essentially, we let the yeast cells do the work for us," Boeke jokes. "They found the right places where a genetic tweak to p53 could bring it back to life."

"To prove that this wasn't just an artifact of our yeast system, we then put the p53 genes back in human cell lines," Boeke says. "They worked well--not quite as well as original p53, but pretty close."

Their findings are published in the April issue of The EMBO (European Molecular Biology Organization) Journal. Other authors were Kexin Yu and Yolanda Eby of Hopkins, and Nikola Pavletich, Ph.D., now at Memorial Sloan-Kettering. Brachmann is now at Washington University in St. Louis.

-JHMI-

Johns Hopkins Medical Institutions' news releases are available on a PRE-EMBARGOED basis on EurekAlert at http://www.eurekalert.org, Newswise at http://www.newswise.com and from the Office of Communications and Public Affairs' direct e-mail news release service. To enroll, call 410-955-4288 or send e-mail to bsimpkin@welchlink.welch.jhu.edu or 76520.560@compuserve.com.

On a POST-EMBARGOED basis find them at http://hopkins.med.jhu.edu, Quadnet at http://www.quad-net.com, ScienceDaily at http://www.sciencedaily.com or on CompuServe in the SciNews-MedNews library of the Journalism Forum under file extension ".JHM".


Story Source:

The above story is based on materials provided by Johns Hopkins Medical Institutions. Note: Materials may be edited for content and length.


Cite This Page:

Johns Hopkins Medical Institutions. "Scientists Fix Mutated Human Tumor-Stopping Gene In Yeast Cells." ScienceDaily. ScienceDaily, 1 April 1998. <www.sciencedaily.com/releases/1998/04/980401124240.htm>.
Johns Hopkins Medical Institutions. (1998, April 1). Scientists Fix Mutated Human Tumor-Stopping Gene In Yeast Cells. ScienceDaily. Retrieved October 30, 2014 from www.sciencedaily.com/releases/1998/04/980401124240.htm
Johns Hopkins Medical Institutions. "Scientists Fix Mutated Human Tumor-Stopping Gene In Yeast Cells." ScienceDaily. www.sciencedaily.com/releases/1998/04/980401124240.htm (accessed October 30, 2014).

Share This



More Health & Medicine News

Thursday, October 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Mind-Controlled Prosthetic Arm Restores Amputee Dexterity

Mind-Controlled Prosthetic Arm Restores Amputee Dexterity

Reuters - Innovations Video Online (Oct. 29, 2014) A Swedish amputee who became the first person to ever receive a brain controlled prosthetic arm is able to manipulate and handle delicate objects with an unprecedented level of dexterity. The device is connected directly to his bone, nerves and muscles, giving him the ability to control it with his thoughts. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Google To Use Nanoparticles, Wearables To Detect Disease

Google To Use Nanoparticles, Wearables To Detect Disease

Newsy (Oct. 29, 2014) Google X wants to improve modern medicine with nanoparticles and a wearable device. It's all an attempt to tackle disease detection and prevention. Video provided by Newsy
Powered by NewsLook.com
Can Drinking Milk Lead To Early Death?

Can Drinking Milk Lead To Early Death?

Newsy (Oct. 29, 2014) Researchers in Sweden released a study showing heavy milk drinkers face an increased mortality risk from a variety of causes. Video provided by Newsy
Powered by NewsLook.com
Obama: The US Will Not 'run and Hide' From Ebola

Obama: The US Will Not 'run and Hide' From Ebola

AP (Oct. 29, 2014) Surrounded by health care workers in the White House East Room, President Barack Obama said the U.S. will likely see additional Ebola cases in the weeks ahead. But he said the nation can't seal itself off in the fight against the disease. (Oct. 29) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins