Jan. 15, 2002 ANN ARBOR, Mich. - When it comes to cooling the burning pain of gastritis or an inflamed stomach lining, reducing the amount of acid in the stomach may seem like a good idea. But two new studies with laboratory mice, conducted by Howard Hughes Medical Institute scientists at the University of Michigan Medical School, indicate it could be exactly the wrong thing to do.
U-M scientists found that antibiotics were the best way to kill the bacteria that cause gastritis and eliminate stomach inflammation in their experimental mice. Mice treated with prescription drugs called proton pump inhibitors or PPIs, which block acid production, acquired more bacteria and developed more inflammatory changes in their stomach linings than untreated mice.
"These animal studies indicate that it is the inflammatory response - triggering the overproduction of hydrochloric acid - which is the stomach's primary response to bacterial colonization," says Juanita L. Merchant, M.D., Ph.D., an HHMI assistant investigator and U-M associate professor of internal medicine and physiology. "Inflammation of the stomach lining coincides with production of peptides called cytokines, which stimulate production of a hormone called gastrin. Gastrin triggers parietal cells in the stomach lining to produce more hydrochloric acid, which kills off most invading microbes. If you inhibit gastric acid production, you interfere with the stomach's natural defense mechanism."
Merchant cautions that without controlled clinical trials, it is impossible to know whether the results would be exactly the same in humans. She also emphasizes that a type of bacteria called Helicobacter pylori, the most common cause of gastritis, was excluded from these studies. Since reduced gastric acidity does appear to make the mammalian stomach more vulnerable to bacterial invasion and gastritis, however, Merchant says physicians may want to re-evaluate the long-term use of omeprazole and other proton-pump-inhibiting drugs in their patients.
Together with Yana Zavros, Ph.D., an HHMI post-doctoral fellow, Merchant and colleagues compared stomach cells from normal mice with those from a strain of transgenic mice, developed at U-M, that lack the gene for producing gastrin. Their goal was to understand the feedback relationship between bacteria, pro-inflammatory factors, hormones and acid secretion in the stomach. Results are published in the January 2002 issues of Gastroenterology and The American Journal of Physiology.
Mice in the U-M studies contracted gastritis just like people do - from eating food or drinking water contaminated with bacteria. While 75 percent of people with gastritis test positive for Helicobacter pylori, many other species of bacteria can trigger inflammatory changes, too, and often co-exist with Helicobacter. No matter what type of bacteria causes the problem, it is a serious medical condition. If untreated, chronic gastritis can lead to peptic ulcers and stomach cancer.
H. pylori is the only bacterial organism in the stomach that cannot be killed by hydrochloric acid. Since Merchant wanted to study the relationship between other bacteria and gastric acid, she needed to exclude the presence of H. pylori. U-M scientists cultured and analyzed bacteria from stomach washings of all normal and gastrin-deficient mice to confirm the absence of Helicobacter. Major types of bacteria identified included Lactobacillus, Enterobacter and Staphylococcus.
U-M scientists treated infected gastrin-deficient mice and normal control mice with antibiotics for 20 days. Other mice were treated for two months with a proton-pump-inhibiting drug called omeprazole or with a combination of omeprazole and antibiotics. At the end of the treatment period, researchers compared cell changes and bacterial counts from the stomach linings of all mice.
Major findings from the U-M studies include:
* Stomach cell samples from both the transgenic gastrin-deficient mice and the normal mice whose ability to produce gastric acid was inhibited by omeprazole all showed significant inflammatory changes -- including more immune cells called lymphocytes -- and greater numbers of bacteria.
* Gastritis that developed in mice on omeprazole resolved after 20 days of antibiotic treatment, despite continued omeprazole treatment and low stomach acidity.
* The number of acid-producing parietal cells and gastrin-secreting G-cells in the stomach increased in all mice with abnormally low levels of hydrochloric acid. Elevated numbers of parietal and G-cells correlated with the presence of inflammation, not with stomach acidity.
* Elevated levels of gastrin during chronic inflammation suppressed production of a growth hormone called somatostatin, which inhibits parietal and G-cell function. When the inflammation subsided following antibiotic treatment, gastrin levels returned to normal releasing the hormonal brake inhibiting somatostatin.
"Our findings show that changes observed in gastrin-deficient mice are caused by inflammation triggered by an overgrowth of many bacterial species," Zavros explains. "An abnormally low level of acidity in the stomach is the factor initiating all these events."
"The bottom-line message is that a two-week course of antibiotics to treat the inflammation is essential for a successful cure," Merchant adds. "Once you get rid of the inflammation, the gastric acid levels should return to normal. It is crucial to take antibiotics for the entire two weeks exactly as your physician has prescribed, however. People often stop taking their medication early or skip doses, which helps the bacteria to develop antibiotic resistance."
In addition to the Howard Hughes Medical Institute, this research was supported by the National Institutes of Health. Linda C. Samuelson, Ph.D., an associate professor of physiology in the Medical School, developed the strain of transgenic mice used in the experiments. Former U-M post-doctoral fellows Gabriele Rieder, Ph.D., and Amy Ferguson, Ph.D., collaborated in the study.
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