Featured Research

from universities, journals, and other organizations

Researchers Create A Simpler Test To Screen Meat For Nerve Tissue Contamination

Date:
March 13, 2003
Source:
University Of Arkansas
Summary:
University of Arkansas researchers have developed a new method for detecting nerve tissue in ground meat, creating a potentially fast and easy way to prevent nerve tissue, potentially infected with bovine spongiform encephalopathy (BSE), from contaminating meat bound for public consumption.

FAYETTEVILLE, Ark. – University of Arkansas researchers have developed a new method for detecting nerve tissue in ground meat, creating a potentially fast and easy way to prevent nerve tissue, potentially infected with bovine spongiform encephalopathy (BSE), from contaminating meat bound for public consumption.

University of Arkansas graduate student Rahul Gangidi, professor of food science Andy Proctor and assistant professor of animal science Fred Pohlman used Fourier Transform Infrared Spectroscopy (FTIR) to examine ground beef contaminated with spinal cord tissue. BSE prions reside in cerebral and spinal tissue. They reported their results in the Journal of Food Science.

BSE has been linked to deaths in Europe from variant Creutzfeldt-Jacob disease in humans who ingested tainted meat. It has not yet been detected in the U.S. meat supply, but it remains a concern to consumers and meat producers alike. Spinal cord tissue can accidentally contaminate meat as it is removed from vertebrate bones close to the spine. The current method used to detect this contamination is a biological assay called ELISA, a complex, lengthy and expensive technique that examines only tiny samples of meat at a time.

Using ELISA to detect nerve tissue in ground meat is like searching for a needle in a haystack by examining a small sample of straw, Gangidi said. "But this new method could be used on the whole haystack."

The U of A scientists decided to investigate attenuated total reflectance FTIR (ATR-FTIR), a form of infrared spectroscopy, because it directly examines the meat sample, is non-destructive, and takes only two minutes to perform. They first looked at differences between spectra obtained from meat and spinal cord to observe differences that could be used to quantify the amount of nerve tissue in meat.

They bought some ground beef at the local grocery store and obtained spinal cord tissue from the University of Arkansas abattoir. They examined the unadulterated meat using ATR-FTIR. They then mixed spinal cord with a certain amount of meat, then added more meat to dilute the amount of spinal cord tissue present in the beef to obtain various levels of adulteration. They then measured the spectra of the various adulterated samples using ATR-FTIR and statistical analysis to find which portions of the spectra could be used to measure adulteration. They found a strong correlation between phosphate groups bound to organic molecules and the presence of bovine spinal cord tissue: As the concentration of spinal cord tissue increased, so did the presence of the specific phosphate group response. This could be due to the presence of sphingomyelin, a component of the sheath that surrounds nerves that is rich in phosphate. The method detected spinal cord contamination down to 0.0016 percent, a vast improvement over the ELISA, which is sensitive to 0.1 percent. Some meat processing plants already have FTIR machines, so this method may become useful in the industry. Further, meat processors could use the technique in a production line, with an IR scanner similar to the "bar code" scanners used in stores today.

The technique needs further development before it becomes useful to industry, Gangidi said.

"However, the potential is there," Proctor said.


Story Source:

The above story is based on materials provided by University Of Arkansas. Note: Materials may be edited for content and length.


Cite This Page:

University Of Arkansas. "Researchers Create A Simpler Test To Screen Meat For Nerve Tissue Contamination." ScienceDaily. ScienceDaily, 13 March 2003. <www.sciencedaily.com/releases/2003/03/030313081310.htm>.
University Of Arkansas. (2003, March 13). Researchers Create A Simpler Test To Screen Meat For Nerve Tissue Contamination. ScienceDaily. Retrieved September 30, 2014 from www.sciencedaily.com/releases/2003/03/030313081310.htm
University Of Arkansas. "Researchers Create A Simpler Test To Screen Meat For Nerve Tissue Contamination." ScienceDaily. www.sciencedaily.com/releases/2003/03/030313081310.htm (accessed September 30, 2014).

Share This



More Health & Medicine News

Tuesday, September 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

How 'Yes Means Yes' Defines Sexual Assault

How 'Yes Means Yes' Defines Sexual Assault

Newsy (Sep. 29, 2014) — Aimed at reducing sexual assaults on college campuses, California has adopted a new law changing the standard of consent for sexual activity. Video provided by Newsy
Powered by NewsLook.com
Scientists May Have Found An Early Sign Of Pancreatic Cancer

Scientists May Have Found An Early Sign Of Pancreatic Cancer

Newsy (Sep. 29, 2014) — Researchers looked at 1,500 blood samples and determined people who developed pancreatic cancer had more branched chain amino acids. Video provided by Newsy
Powered by NewsLook.com
Colo. Doctors See Cluster of Enterovirus Cases

Colo. Doctors See Cluster of Enterovirus Cases

AP (Sep. 29, 2014) — Doctors at the Children's Hospital of Colorado say they have treated over 4,000 children with serious respiratory illnesses since August. Nine of the patients have shown distinct neurological symptoms, including limb weakness. (Sept. 29) Video provided by AP
Powered by NewsLook.com
Dr.'s Unsure of Cause of Fast-Spreading Virus

Dr.'s Unsure of Cause of Fast-Spreading Virus

AP (Sep. 29, 2014) — Doctors at the Children's Hospital of Colorado say they have treated over 4,000 children with serious respiratory illnesses since August. Nine of the patients have shown distinct neurological symptoms, including limb weakness. (Sept. 29) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins