Featured Research

from universities, journals, and other organizations

Temple Study Identifies Molecular Mechanisms That May Assist Gene In Blocking Lung Cancer

Date:
October 9, 2003
Source:
Temple University
Summary:
The molecular mechanisms that may assist the tumor suppressing gene Rb2/p130 in blocking the progression of lung cancer cells has been clearly identified for the first time according to a study by researchers at Temple University's Sbarro Institute for Cancer Research and Molecular Medicine.

The molecular mechanisms that may assist the tumor suppressing gene Rb2/p130 in blocking the progression of lung cancer cells has been clearly identified for the first time according to a study by researchers at Temple University's Sbarro Institute for Cancer Research and Molecular Medicine.

Related Articles


The results of their study, "pRb2/p130 target genes in non-small cell lung cancer cells identified by microarray analysis," appear in the Oct. 9 issue of Oncogene (http://www.nature.com/onc/).

The study, led by Antonio Giordano, M.D., Ph.D., head of the Sbarro Institute at Temple, was a follow-up to previous experiments led by Giordano that demonstrated an inverse relationship between Rb2 expression and the aggressiveness of lung cancer. Giordano discovered the Rb2 gene while working at Temple's Fels Cancer Institute in the early 1990s. In those earlier studies, correct copies of Rb2 were introduced into mice with lung tumors using a viral system developed by Pier Paolo Claudio, M.D., Ph.D., associate professor of biotechnology at the Sbarro Institute. When the Rb2 gene was over-expressed in the cancer cells, it caused the tumors in the mice to completely regress(http://jncicancerspectrum.oupjournals.org/jnci/content/vol90/issue19).

"In this study, we wanted to understand the molecular mechanisms behind Rb2/p130 tumor growth inhibition," says Giuseppe Russo, Ph.D., a post doctoral fellow at the Sbarro Institute and the study's lead author. "We wanted to know what this gene does in lung cancer cells, what other genes it may target, and how it controls cell regulation.

"It's like this molecular process of tumor regression is a movie and Rb2 is the lead actor," Giordano adds. "What we wanted to know is who the supporting cast is."

After introducing correct copies of Rb2 into H23 lung cancer cells via Claudio's viral shuttle, the researchers used customized microarray analysis to examine the simultaneous expression of thousands of genes within the cancer cell. Microarray is a powerful new tool that allows researchers to study the molecular basis of interactions on a scale that had been impossible using conventional methods.

"Through the use of the microarray, we were able to see which genes were over-expressed or under-expressed because of the enhanced Rb2 gene expression," says Giordano. "This is the first time that we have been able to clearly identify the genes that were being regulated by the Rb2 expression in lung cancer cells."

Some of the nearly 70 regulated genes that were identified through this study were previously known to be involved in the progression of lung cancer, says Giordano, meaning that the researchers were able to confirm previously published data. "But many of the identified genes were not previously known to be involved in lung cancer or be regulated by Rb2," he adds. The Temple researchers believe that identifying these genes could play an important role in developing future gene therapies to diagnose and treat lung cancer.

"Any regulation in a gene that is found to be caused by Rb2 is important to us because we can then study why that gene is regulated and use that information to develop molecular therapeutic approaches to lung cancer," says Giordano. "The gene expression profiles provided by microarray analysis will someday help tailor specific gene therapies to individual patients." Lung cancer is the leading cause of cancer death worldwide and is usually diagnosed at an incurable stage, most likely due to the absence of effective therapies as well as standard diagnostic procedures of early tumoral stages when compared with other cancers types, such as colon, breast and prostate cancers.

The study was funded by the National Institutes of Health and the Sbarro Health Research Organization.


Story Source:

The above story is based on materials provided by Temple University. Note: Materials may be edited for content and length.


Cite This Page:

Temple University. "Temple Study Identifies Molecular Mechanisms That May Assist Gene In Blocking Lung Cancer." ScienceDaily. ScienceDaily, 9 October 2003. <www.sciencedaily.com/releases/2003/10/031009064623.htm>.
Temple University. (2003, October 9). Temple Study Identifies Molecular Mechanisms That May Assist Gene In Blocking Lung Cancer. ScienceDaily. Retrieved December 17, 2014 from www.sciencedaily.com/releases/2003/10/031009064623.htm
Temple University. "Temple Study Identifies Molecular Mechanisms That May Assist Gene In Blocking Lung Cancer." ScienceDaily. www.sciencedaily.com/releases/2003/10/031009064623.htm (accessed December 17, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Wednesday, December 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

AFP (Dec. 17, 2014) Border closures, quarantines and crop losses in West African nations battling the Ebola virus could lead to as many as one million people going hungry, UN food agencies said on Wednesday. Duration: 00:52 Video provided by AFP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
Flu Outbreak Closing Schools in Ohio

Flu Outbreak Closing Schools in Ohio

AP (Dec. 17, 2014) A wave of flu illnesses has forced some Ohio schools to shut down over the past week. State officials confirmed one pediatric flu-related death, a 15-year-old girl in southern Ohio. (Dec. 17) Video provided by AP
Powered by NewsLook.com
Yoga Could Be As Beneficial For The Heart As Walking, Biking

Yoga Could Be As Beneficial For The Heart As Walking, Biking

Newsy (Dec. 17, 2014) Yoga can help your weight, blood pressure, cholesterol and heart just as much as biking and walking does, a new study suggests. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins