Featured Research

from universities, journals, and other organizations

Wnt Signalling Protein Dishevelled Acts In The Nucleus, Not Just In The Cytoplasm

Date:
February 22, 2005
Source:
BioMed Central
Summary:
Researchers have identified that Dishevelled doesn't only function in the cytoplasm and at the cell membrane – it must also pass into the nucleus. A study published today in Journal of Biology reveals that Dishevelled, a key player in the Wnt/beta-catenin signalling pathway, has to be localised in the nucleus to perform a key aspect of its function.

Researchers have identified that Dishevelled doesn't only function in the cytoplasm and at the cell membrane – it must also pass into the nucleus. A study published today in Journal of Biology reveals that Dishevelled, a key player in the Wnt/beta-catenin signalling pathway, has to be localised in the nucleus to perform a key aspect of its function. This discovery should shed light on both normal embryonic development and the development of cancer.

In the paper, Sergei Sokol and colleagues, from Harvard Medical School, show that Dishevelled (Dsh) is constantly shuttling between the nucleus and the cytoplasm, owing to its nuclear export and import sequences, but that it has to be in the nucleus to respond to certain Wnt signals transmitted through the 'canonical' Wnt signalling pathway.

Dsh has always been considered to be a cytoplasmic protein, exerting its function as a stabiliser of beta-catenin in the cell cytoplasm after stimulation by Wnt secreted ligands binding to Frizzled receptors on the cell surface.

Sokol and colleagues show using Xenopus embryos and mammalian cultured cells that a mutated version of Dsh, which accumulates in the nucleus, is functional in the Wnt signalling pathway. Preventing Dsh from getting into the nucleus, however, either by mutating the nuclear localisation signal of the protein or by using a drug that disables the nuclear export machinery, impairs function. In mammalian cells, endogenous Dsh responds to Wnt ligands by mobilising to the nucleus.

"Our findings are consistent with a scenario in which Wnt signaling may cause nuclear translocation of Dsh followed by formation of a stable beta-catenin/Tcf3 complex and transcriptional activation of target genes", explain the authors. Dsh's exact role in the nucleus, however, is still unclear.

Dsh is also important in non-canonical Wnt signaling pathways, which involve proteins such as Rho GTPase and JNK. Sokol and colleagues show that nuclear localisation of Dsh is not required for its function in non-canonical signaling.

###

This press release is based on the following article:

Nuclear localization is required for Dishevelled function in Wnt/beta-catenin signalingKeiji Itoh, Barbara K Brott, Gyu-Un Bae, Marianne J Ratcliffe and Sergei Y Sokol

Journal of Biology 2005, 4:3 (15 February 2005)


Story Source:

The above story is based on materials provided by BioMed Central. Note: Materials may be edited for content and length.


Cite This Page:

BioMed Central. "Wnt Signalling Protein Dishevelled Acts In The Nucleus, Not Just In The Cytoplasm." ScienceDaily. ScienceDaily, 22 February 2005. <www.sciencedaily.com/releases/2005/02/050218132822.htm>.
BioMed Central. (2005, February 22). Wnt Signalling Protein Dishevelled Acts In The Nucleus, Not Just In The Cytoplasm. ScienceDaily. Retrieved July 22, 2014 from www.sciencedaily.com/releases/2005/02/050218132822.htm
BioMed Central. "Wnt Signalling Protein Dishevelled Acts In The Nucleus, Not Just In The Cytoplasm." ScienceDaily. www.sciencedaily.com/releases/2005/02/050218132822.htm (accessed July 22, 2014).

Share This




More Matter & Energy News

Tuesday, July 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Government Approves East Coast Oil Exploration

Government Approves East Coast Oil Exploration

AP (July 18, 2014) The Obama administration approved the use of sonic cannons to discover deposits under the ocean floor by shooting sound waves 100 times louder than a jet engine through waters shared by endangered whales and turtles. (July 18) Video provided by AP
Powered by NewsLook.com
Sunken German U-Boat Clearly Visible For First Time

Sunken German U-Boat Clearly Visible For First Time

Newsy (July 18, 2014) The wreckage of the German submarine U-166 has become clearly visible for the first time since it was discovered in 2001. Video provided by Newsy
Powered by NewsLook.com
Obama: U.S. Must Have "smartest Airports, Best Power Grid"

Obama: U.S. Must Have "smartest Airports, Best Power Grid"

Reuters - US Online Video (July 17, 2014) President Barak Obama stopped by at a lunch counter in Delaware before making remarks about boosting the nation's infrastructure. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com
Crude Oil Prices Bounce Back After Falling Below $100 a Barrel

Crude Oil Prices Bounce Back After Falling Below $100 a Barrel

TheStreet (July 16, 2014) Oil Futures are bouncing back after tumbling below $100 a barrel for the first time since May yesterday. Jeff Grossman is the president of BRG Brokerage and trades at the NYMEX. Grossman tells TheStreet the Middle East is always a concern for oil traders. Oil prices were pushed down in recent weeks on Libya increasing its production. Supply disruptions in Iraq fading also contributed to prices falling. News from China's economic front showing a growth for the second quarter also calmed fears on its slowdown. Jeff Grossman talks to TheStreet's Susannah Lee on this and more on the Energy Department's Energy Information Administration (EIA) report. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins