Featured Research

from universities, journals, and other organizations

Where's Waldo's DNA? New NIST SRM Joins Search

Date:
April 14, 2005
Source:
National Institute Of Standards And Technology
Summary:
A new reference standard from the National Institute of Standards and Technology (NIST) may help genetics labs develop improved methods of searching for a mutant needle in a DNA haystack.

NIST principal investigator Barbara Levin (left) observes while NIST research chemist Diane Hancock pipettes a sample in preparation for an analysis of low-frequency mitochrondrial DNA mutations.
Credit: Photo by Barry Gardner

A new reference standard from the National Institute of Standards and Technology (NIST) may help genetics labs develop improved methods of searching for a mutant needle in a DNA haystack.

Related Articles


A single DNA molecule carrying part of a person’s genetic code is a chain of basic chemical units called nucleotides. The number of nucleotides can range from about 16,500 in mitochondrial DNA (mtDNA) to several million in nuclear DNA. A key mutation in a DNA strand may involve only a single nucleotide and yet cause serious health effects.

Accurate analysis of mitochondrial DNA (mtDNA), either for forensic identification or for studying genetic-based diseases, often hinges on the ability to detect such mutations that occur only infrequently, even in the same individual. Unlike the cell's nuclear DNA, a person's mtDNA is often heteroplasmic—a mix of a dominant DNA sequence with fewer mutated sequences that differ from the dominant version by one or more nucleotides. There are hundreds or thousands of mitochondria in cells, and the exact percentage of the minority mtDNA in the mix can vary dramatically in an individual from tissue to tissue and even from cell to cell. In general, it can be very difficult to identify variants that make up less than 20 percent of the sample unless you already know they are there.

Researchers face a similar problem of detecting low-frequency variants when analyzing pooled samples of nuclear DNA from a population of individuals in the hope of identifying specific mutations responsible for genetic diseases.

To help the research community develop and test more sensitive techniques for detecting low-frequency mutations in heteroplasmic DNA, NIST researchers have developed a new Standard Reference Material, SRM 2394, “Heteroplasmic Mitochondrial DNA Mutation Detection Standard.” The new material is a set of mixtures, at 10 different certified concentrations, of two DNA fragments that differ from each other at only one position.


Story Source:

The above story is based on materials provided by National Institute Of Standards And Technology. Note: Materials may be edited for content and length.


Cite This Page:

National Institute Of Standards And Technology. "Where's Waldo's DNA? New NIST SRM Joins Search." ScienceDaily. ScienceDaily, 14 April 2005. <www.sciencedaily.com/releases/2005/04/050411203431.htm>.
National Institute Of Standards And Technology. (2005, April 14). Where's Waldo's DNA? New NIST SRM Joins Search. ScienceDaily. Retrieved October 24, 2014 from www.sciencedaily.com/releases/2005/04/050411203431.htm
National Institute Of Standards And Technology. "Where's Waldo's DNA? New NIST SRM Joins Search." ScienceDaily. www.sciencedaily.com/releases/2005/04/050411203431.htm (accessed October 24, 2014).

Share This



More Health & Medicine News

Friday, October 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Breakfast Debate: To Eat Or Not To Eat?

Breakfast Debate: To Eat Or Not To Eat?

Newsy (Oct. 23, 2014) Conflicting studies published in the same week re-ignited the debate over whether we should be eating breakfast. Video provided by Newsy
Powered by NewsLook.com
Ebola Fears Keep Guinea Hospitals Empty

Ebola Fears Keep Guinea Hospitals Empty

AP (Oct. 23, 2014) Fears of Ebola are keeping doctors and patients alike away from hospitals in the West African nation of Guinea. (Oct. 23) Video provided by AP
Powered by NewsLook.com
Despite Rising Death Toll, Many Survive Ebola

Despite Rising Death Toll, Many Survive Ebola

AP (Oct. 23, 2014) The family of a Dallas nurse infected with Ebola in the US says doctors can no longer detect the virus in her. Despite the mounting death toll in West Africa, there are survivors there too. (Oct. 23) Video provided by AP
Powered by NewsLook.com
Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Orthodontist Mom Jennifer Salzer on the Best Time for Braces

Working Mother (Oct. 22, 2014) Is your child ready? Video provided by Working Mother
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins