Featured Research

from universities, journals, and other organizations

Fish And Chips: A Fast Track To Understanding Blood Development

Date:
July 18, 2005
Source:
Public Library of Science
Summary:
Microarray studies are combined with a functional screen in zebrafish to identify new genes that are involved in the function and differentiation of hematopoietic stem cells in a study published in the open access journal PLoS Biology.

Using microarray technology and zebrafish, researchers discovered which human genes are active during hematopoiesis, the formation of blood cells.
Credit: Photo : Verfaillie et al.

What do you get when you mix RNA chips and zebrafish? A new two-stage method for studying human gene expression differs in human stem cells and progenitor cells, according to a new article in the open access journal PLoS Biology. In the paper, Catherine Verfaillie and colleagues identify 277 human genes with different gene expression profiles in stem and progenitor cells involved in blood cell development, or hematopoiesis. The paper also suggests that a developmental regulator called fibroblast growth factor may play a role in hematopoiesis.

Related Articles


The new method may also have relevance beyond the field of blood development. "This could be done not only for hematopoietic stem cells as is done here but also for brain, pancreas, endothelium, smooth and cardiac muscle, because of the high homology between man and fish in development of these tissues," explains Verfaillie.

The RNA transcript microarray, or "RNA chip," allowed Verfaillie and colleagues to compare gene expression in cell cultures with many or few hematopoietic stem cells. Previously, the authors had developed a method to harvest stem cells using bone marrow and blood from umbilical cords. Sixty-one of the 277 genes found to have different expressions profiles in stem and progenitor cells had no known function in human hematopoietic stem cells, though the genes do resemble genes found in zebrafish. Fish with mutated versions of fourteen of these genes developed blood disorders, suggesting that the genes are necessary for normal hematopoiesis. Three of the 14 genes are known to play a role in fibroblast growth factor signaling. In the future, researchers may learn the specific relationship between fibroblast growth factor and hematopoiesis.

But Verfaillie stresses that the study has implications for researchers in various fields. "This is one of the first examples in the literature where high-throughput gene expression analysis using gene arrays has been functionally validated by high-throughput true functional analysis in a model organism," says Verfaillie. With this approach, new studies may zero in on genes with central roles in other kinds of human development.

###

Citation: Eckfeldt CE, Mendenhall EM, Flynn CM, Wang TF, Pickart MA, et al. (2005) Functional analysis of human hematopoietic stem cell gene expression using zebrafish. PLoS Biol 3(8): e254.


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Cite This Page:

Public Library of Science. "Fish And Chips: A Fast Track To Understanding Blood Development." ScienceDaily. ScienceDaily, 18 July 2005. <www.sciencedaily.com/releases/2005/07/050710102257.htm>.
Public Library of Science. (2005, July 18). Fish And Chips: A Fast Track To Understanding Blood Development. ScienceDaily. Retrieved March 4, 2015 from www.sciencedaily.com/releases/2005/07/050710102257.htm
Public Library of Science. "Fish And Chips: A Fast Track To Understanding Blood Development." ScienceDaily. www.sciencedaily.com/releases/2005/07/050710102257.htm (accessed March 4, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Wednesday, March 4, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Rare Goblin Shark Found in Australia

Rare Goblin Shark Found in Australia

AFP (Mar. 3, 2015) A goblin shark, a rare sea creature described as an &apos;alien of the deep&apos; is found off Australia and delivered to the Australian Museum in Sydney. Duration: 01:25 Video provided by AFP
Powered by NewsLook.com
500 Snakes Surprise Construction Workers In Canada

500 Snakes Surprise Construction Workers In Canada

Newsy (Mar. 2, 2015) Hundreds of snakes, disturbed by a construction project, were relocated to a wildlife rescue association in Canada. Video provided by Newsy
Powered by NewsLook.com
Going Gluten-Free Could Get You A Tax Break

Going Gluten-Free Could Get You A Tax Break

Newsy (Mar. 2, 2015) If a doctor advises you to remove gluten from your diet, you could get a tax deduction on the amount you spend on gluten-free foods. Video provided by Newsy
Powered by NewsLook.com
Zookeepers Copy Animal Poses In Hilarious Viral Photos

Zookeepers Copy Animal Poses In Hilarious Viral Photos

Buzz60 (Mar. 2, 2015) Zookeepers at the Symbio Wildlife Park in Helensburgh, Australia decided to take some of their favorite animal photos and recreate them by posing just like the animals. Jen Markham (@jenmarkham) has the story. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins