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Researchers Reveal Secret Of Key Protein In Brain And Heart Function

Date:
August 17, 2005
Source:
Brown University
Summary:
Brown University researchers have solved the structure of a critical piece of SAP97, a protein used to keep hearts beating and brains learning. Results, reported by Dale Mierke in the Journal of Biological Chemistry, put science a step closer to understanding how this protein aids in brain and heart function.
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FULL STORY

Synapse-associated protein 97: The model shows the PDZ domain of the SAP97 protein in a ribbon format, highlighting its structural elements. The intra-cellular portion of the NMDA receptor is shown as ball-and-stick format atoms. SAP97 is a scaffolding protein, facilitating nerve signals.
Credit: Image courtesy of Brown University

PROVIDENCE, R.I. — Brown University biologists have solved the structure of a critical piece of synapse-associated protein 97 (SAP97) found in abundance in the heart and head, where it is believed to play a role in everything from cardiac contractions to memory creation. Results are published in The Journal of Biological Chemistry.

Dale Mierke, associate professor of medical science at Brown, said that knowing how a piece of SAP97 is built is an important step. Now that part of the protein’s structure is solved, scientists can create a molecule to disable it. That, in turn, will allow them to fully understand SAP97’s role in the body. And that will point drug makers to targets for developing new ways to treat cardiac or neurological diseases.

“To arrive at a solution, you need to understand the problem,” Mierke said. “Solving protein structures opens doors for effective treatments.”

SAP97 is found mainly in the central nervous system and is known as a “scaffolding” protein. In this role, it serves as a sort of tether, grabbing proteins inside the cell critical to nerve signaling and keeping them close to N-methyl-D-asparate (NMDA) receptors at the cell surface. NMDA receptors help usher in a neurotransmitter called glutamate that is essential for learning and memory and also plays a role in drug addiction. A similar scaffolding mechanism is at work in the heart, where it affects basic functions, including the heartbeat.

SAP97 is a complex protein made up of five “domains” similar to a train comprised of an engine and four boxcars. In their experiments, Mierke, graduate student Lei Wang and postdoctoral research fellow Andrea Piserchio – all colleagues in the Department of Molecular Pharmacology, Physiology and Biotechnology – focused on the engine. This domain, known as PDZ1, is where the protein links to NMDA receptors. The team used high-resolution nuclear magnetic resonance spectroscopy to solve the structure of PDZ1, as well as a small portion of the receptor to which it binds.

Mierke said the group is now developing a molecule that can inhibit PDZ1 as well as PDZ2, the first boxcar on the multi-domain protein.

The National Institutes of Health funded the work.


Story Source:

The above story is based on materials provided by Brown University. Note: Materials may be edited for content and length.


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Brown University. "Researchers Reveal Secret Of Key Protein In Brain And Heart Function." ScienceDaily. ScienceDaily, 17 August 2005. <www.sciencedaily.com/releases/2005/07/050730093601.htm>.
Brown University. (2005, August 17). Researchers Reveal Secret Of Key Protein In Brain And Heart Function. ScienceDaily. Retrieved April 25, 2015 from www.sciencedaily.com/releases/2005/07/050730093601.htm
Brown University. "Researchers Reveal Secret Of Key Protein In Brain And Heart Function." ScienceDaily. www.sciencedaily.com/releases/2005/07/050730093601.htm (accessed April 25, 2015).

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