Featured Research

from universities, journals, and other organizations

Cell Cultures Can Sort Out CJD And Scrapie Infectious Agents

Date:
October 22, 2005
Source:
Yale University
Summary:
Research at Yale University School of Medicine shows that infection with a weak strain of Creutzfeldt-Jacob Disease (CJD) prevents infection by more virulent strains and that the protection requires persistent replication by the infectious agent, but not misfolded prions. Protection with a weak animal agent may account for the low incidence of CJD linked to Mad Cow Disease in people.

Research in Japan and at Yale University School of Medicine shows that infection with a weak strain of Creutzfeldt-Jacob Disease (CJD) prevents infection by more virulent strains and that the protection requires persistent replication by the infectious agent, but not misfolded prions.

The "prion diseases" including Mad Cow Disease, scrapie and CJD have been in recent news because of their devastating effects on the brain and concern about transmission of the infectious agents. In prior research on animals, a weaker strain of CJD protected against a more destructive strain.

This study, reported in the journal Science, duplicated and extended results of the animal experiments in a neural cell culture assay. In culture assays it was possible to test human and sheep agent strain combinations that could not be discriminated in animals. These studies showed that a persistent protective CJD infection did not require cells from the immune system or misfolded prions. Cells infected with a weak strain of CJD were also protected from infection by two strains of sheep scrapie agent.

"We demonstrate a new and very sensitive assay for infection by these agents that can discriminate among different strains, such as those that cause sheep scrapie and human CJD," said senior author Laura Manuelidis, professor and section chief of Surgery/Neuropathology at Yale.

According to Manuelidis protection with a weak animal agent may account for the low incidence of CJD linked to Mad Cow Disease in people. "Our plan is to use these rapid infectivity assays to identify the different agents -- including those linked to Mad Cow Disease -- on the molecular as well as biological levels," said Manuelidis.

"This, as well as our previous results showing that most of the abnormal prion protein can be separated from infectious particles, point to a virus as the causal agent." said Manuelidis "These results are not consistent with the idea that abnormal forms of the prion protein are infectious."

###

Co-authors on the paper are Yale visiting professor Noriuki Nishida of Gifu University and Sherigeru Katamine of the Nagasaki University Graduate School of Medicine in Japan. The research was funded by the National Institute Neurological Disorders and Stroke and the Department of Defense National Prion Research Program.

Citation: Science 310: (October 21, 2005)


Story Source:

The above story is based on materials provided by Yale University. Note: Materials may be edited for content and length.


Cite This Page:

Yale University. "Cell Cultures Can Sort Out CJD And Scrapie Infectious Agents." ScienceDaily. ScienceDaily, 22 October 2005. <www.sciencedaily.com/releases/2005/10/051022111510.htm>.
Yale University. (2005, October 22). Cell Cultures Can Sort Out CJD And Scrapie Infectious Agents. ScienceDaily. Retrieved April 23, 2014 from www.sciencedaily.com/releases/2005/10/051022111510.htm
Yale University. "Cell Cultures Can Sort Out CJD And Scrapie Infectious Agents." ScienceDaily. www.sciencedaily.com/releases/2005/10/051022111510.htm (accessed April 23, 2014).

Share This



More Plants & Animals News

Wednesday, April 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Monkeys Are Better At Math Than We Thought, Study Shows

Monkeys Are Better At Math Than We Thought, Study Shows

Newsy (Apr. 23, 2014) A Harvard University study suggests monkeys can use symbols to perform basic math calculations. Video provided by Newsy
Powered by NewsLook.com
Raw: Leopard Bites Man in India

Raw: Leopard Bites Man in India

AP (Apr. 22, 2014) A leopard caused panic in the city of Chandrapur on Monday when it sprung from the roof of a house and charged at rescue workers. (April 22) Video provided by AP
Powered by NewsLook.com
Iowa College Finds Beauty in Bulldogs

Iowa College Finds Beauty in Bulldogs

AP (Apr. 22, 2014) Drake University hosts 35th annual Beautiful Bulldog Contest. (April 21) Video provided by AP
Powered by NewsLook.com
805-Pound Shark Caught Off The Coast Of Florida

805-Pound Shark Caught Off The Coast Of Florida

Newsy (Apr. 22, 2014) One Florida fisherman caught a 805-pound shark off the coast of Florida earlier this month. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins