Featured Research

from universities, journals, and other organizations

Boston University Scientists Develop New Application To Characterize Structure Of DNA Molecules

Date:
March 2, 2006
Source:
Boston University
Summary:
A team of researchers from Boston University has developed a new application to enable more precise measurement of the location of a fluorescent label in a DNA layer. The new technique, called spectral self-interference fluorescence microscopy (SSFM), maps the interference spectrum from a fluorophore located on a layered reflecting surface into a position with sub-nanometer accuracy.

A team of researchers from Boston University has developed a new application to enable more precise measurement of the location of a fluorescent label in a DNA layer. According to their study, published in a recent issue of Proceedings of the National Academy of Sciences, the new technique provides insight into the shape of DNA molecules attached to a surface, such as microarrays used in genomics research. Determining specific information about how surface-bound DNA molecules conform may significantly improve the efficiency of DNA hybridization and microarray technology and thus impact emerging clinical and biotechnological fields.

The technique, called spectral self-interference fluorescence microscopy (SSFM), maps the interference spectrum from a fluorophore (fluorescent molecule) label located on a layered reflecting surface into a position with sub-nanometer accuracy. "Although a number of other methods have been used to determine the structure of the DNA layer, they are not very sensitive to variations in the shape of DNA molecules," said Bennett Goldberg, professor of physics and study co-author. "Our group has developed SSFM to determine the precise measurement of the location of a fluorescent label relative to the microarray surface which provides us with specific information about the conformation of DNA molecules."

Using SSFM, the team estimated the shape of coiled single-stranded DNA, the average tilt of double-stranded DNA of different lengths, and estimated the amount of hybridization. The data provide important new proof points for the capabilities of novel optical surface analysis methods of the behavior of DNA on microarray surfaces.

"Determining DNA conformation and hybridization behavior provide the information required to move DNA interfacial applications forward," said M. Selim Unlu, electrical and computer engineering professor and study co-author. "Our research shows that locating a fluorescent label attached to a certain position within a DNA chain offers highly accurate information about the shape of DNA molecules bound to the surface of a microarray."

Additional study investigators include Dr. Lev Moiseev, electrical and computer engineering research associate; Anna K. Swan, associate professor of electrical and computer engineering; and Charles R. Cantor, professor of biomedical engineering and co-director of the Center for Advanced Biotechnology at BU.

###

Founded in 1839, Boston University is an internationally recognized institution of higher education and research. With more than 30,000 students, it is the fourth largest independent university in the United States. BU contains 17 colleges and schools along with a number of multi-disciplinary centers and institutes which are central to the school's research and teaching mission.


Story Source:

The above story is based on materials provided by Boston University. Note: Materials may be edited for content and length.


Cite This Page:

Boston University. "Boston University Scientists Develop New Application To Characterize Structure Of DNA Molecules." ScienceDaily. ScienceDaily, 2 March 2006. <www.sciencedaily.com/releases/2006/02/060228175655.htm>.
Boston University. (2006, March 2). Boston University Scientists Develop New Application To Characterize Structure Of DNA Molecules. ScienceDaily. Retrieved August 22, 2014 from www.sciencedaily.com/releases/2006/02/060228175655.htm
Boston University. "Boston University Scientists Develop New Application To Characterize Structure Of DNA Molecules." ScienceDaily. www.sciencedaily.com/releases/2006/02/060228175655.htm (accessed August 22, 2014).

Share This




More Matter & Energy News

Friday, August 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Former TSA X-Ray Scanners Easily Tricked To Miss Weapons

Former TSA X-Ray Scanners Easily Tricked To Miss Weapons

Newsy (Aug. 21, 2014) — Researchers found the scanners could be duped simply by placing a weapon off to the side of the body or encasing it under a plastic shield. Video provided by Newsy
Powered by NewsLook.com
Flower Power! Dandelions Make Car Tires?

Flower Power! Dandelions Make Car Tires?

Reuters - Business Video Online (Aug. 20, 2014) — Forget rolling on rubber, could car drivers soon be traveling on tires made from dandelions? Teams of scientists are racing to breed a type of the yellow flower whose taproot has a milky fluid with tire-grade rubber particles in it. As Joanna Partridge reports, global tire makers are investing millions in research into a new tire source. Video provided by Reuters
Powered by NewsLook.com
Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Awesome New Camouflage Sheet Was Inspired By Octopus Skin

Newsy (Aug. 19, 2014) — Scientists have developed a new device that mimics the way octopuses blend in with their surroundings to hide from dangerous predators. Video provided by Newsy
Powered by NewsLook.com
Researcher Testing on-Field Concussion Scanners

Researcher Testing on-Field Concussion Scanners

AP (Aug. 19, 2014) — Four Texas high school football programs are trying out an experimental system designed to diagnose concussions on the field. The technology is in response to growing concern over head trauma in America's most watched sport. (Aug. 19) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins