Featured Research

from universities, journals, and other organizations

Making A Face: A New And Earlier Marker Of Neural Crest Development

Date:
July 11, 2006
Source:
Yale University
Summary:
The fate of cells that go on to form the face, skull and nerve centers of the head and neck in vertebrates is determined much earlier in development than previously thought, and is independent of interaction with other forming tissues, according to a recent study published in the journal Nature.

Early chick embryo with Pax-7 (red) designating neural crest progenitor cells.
Credit: Martin Garcia-Castro

The fate of cells that go on to form the face, skull and nerve centers of the head and neck in vertebrates is determined much earlier in development than previously thought, and is independent of interaction with other forming tissues, according to a recent study published in the journal Nature.

The collaborators at Yale and Caltech demonstrate with three different technologies -- immunostaining of proteins, in situ hybridization and multiplex RT-PCR of mRNAs -- that formation of neural crest cells in chick embryos is independent of both mesoderm and neural tissues. They also identify, Pax7, as an early marker of neural crest formation and prove that its function is required in the earliest stages of development.

The neural crest is a population of stem cells that migrate extensively during development and give rise to many derivatives, including most of the bone and cartilage of the head skeleton, pigment cells of the skin, and cells of the peripheral nervous system.

In humans, cleft palate, heart valve malformations and various tumors are among the common malformations associated with disruption of neural crest development.

Chick embryos have well-characterized stages and are a valuable model for examining vertebrate development. While it was known that the ability to form neural crest cells declines after "stage 10," the researchers were seeking the earliest conditions surrounding formation of these important stem cells.

"Understanding the origin of neural crest cells -- where, when and how they arise -- is a critical step if we are to manipulate them for therapeutic purposes," said Martín García-Castro, assistant professor of molecular, cellular and developmental biology at Yale and principal investigator on the study. "Implications of these basic questions of biology and development reach far beyond these chicken and eggs."

Based on work from the 1940's before molecular tools were available, the neural crest was thought to form by interactions between neural and non-neural cell layers. "We show in this work that neural crest stem cell precursors are designated very early in development -- as early as the gastrula stages -- and in an independent fashion from those other tissues," said Martín García-Castro.

The researchers grew grafts of cells from "stage 3" chick embryos, before the neural plate formed, in non-inducing cultures. Surprisingly, restricted regions of the embryo generated both migrating neural crest cells and their derivative cell types, without any interaction with neural or mesodermal tissues.

"Our results are contrary to current text-book models and suggest that different modes of neural crest induction operate during development," said Martín García-Castro. "Interestingly, the one we have uncovered is related to the early, cranial neural crest cells, the only ones in higher vertebrates that retain bone and cartilage forming potential."

Other authors were Martin L. Basch and Marianne Bronner-Fraser for the California Institute of Technology. The research was supported by a US Public Health Service grant.


Story Source:

The above story is based on materials provided by Yale University. Note: Materials may be edited for content and length.


Cite This Page:

Yale University. "Making A Face: A New And Earlier Marker Of Neural Crest Development." ScienceDaily. ScienceDaily, 11 July 2006. <www.sciencedaily.com/releases/2006/07/060711132450.htm>.
Yale University. (2006, July 11). Making A Face: A New And Earlier Marker Of Neural Crest Development. ScienceDaily. Retrieved April 17, 2014 from www.sciencedaily.com/releases/2006/07/060711132450.htm
Yale University. "Making A Face: A New And Earlier Marker Of Neural Crest Development." ScienceDaily. www.sciencedaily.com/releases/2006/07/060711132450.htm (accessed April 17, 2014).

Share This



More Plants & Animals News

Thursday, April 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

The Great British Farmland Boom

The Great British Farmland Boom

Reuters - Business Video Online (Apr. 17, 2014) — Britain's troubled Co-operative Group is preparing to cash in on nearly 18,000 acres of farmland in one of the biggest UK land sales in decades. As Ivor Bennett reports, the market timing couldn't be better, with farmland prices soaring over 270 percent in the last 10 years. Video provided by Reuters
Powered by NewsLook.com
Flamingo Frenzy Ahead of Zoo Construction

Flamingo Frenzy Ahead of Zoo Construction

AP (Apr. 17, 2014) — With plenty of honking, flapping, and fluttering, more than three dozen Caribbean flamingos at Zoo Miami were rounded up today as the iconic exhibit was closed for renovations. (April 17) Video provided by AP
Powered by NewsLook.com
Change of Diet Helps Crocodile Business

Change of Diet Helps Crocodile Business

Reuters - Business Video Online (Apr. 16, 2014) — Crocodile farming has been a challenge in Zimbabwe in recent years do the economic collapse and the financial crisis. But as Ciara Sutton reports one of Europe's biggest suppliers of skins to the luxury market has come up with an unusual survival strategy - vegetarian food. Video provided by Reuters
Powered by NewsLook.com
Could Even Casual Marijuana Use Alter Your Brain?

Could Even Casual Marijuana Use Alter Your Brain?

Newsy (Apr. 16, 2014) — A new study conducted by researchers at Northwestern and Harvard suggests even casual marijuana use can alter your brain. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins