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New Microchip For PCR Testing At Crime Scenes, Doctors' Offices

Date:
January 24, 2008
Source:
American Chemical Society
Summary:
Researchers in Hong Kong have miniaturized technology needed to perform the versatile polymerase chain reaction (PCR) -- widely used in criminal investigations, disease diagnosis, and a range of other key applications. They report development of a long-sought PCR microchip that could permit use of PCR at crime scenes, in doctors' offices, and other out-of-lab locations.

This tiny microchip (center, attached to pipette tips) could give researchers the ability to analyze DNA at crime scenes, doctor's offices and other out-of-lab locations.
Credit: I-Ming Hsing, the Hong Kong University of Science and Technology

Researchers in Hong Kong have miniaturized technology needed to perform the versatile polymerase chain reaction (PCR) -- widely used in criminal investigations, disease diagnosis, and a range of other key applications. They report development of a long-sought PCR microchip that could permit use of PCR at crime scenes, in doctors' offices, and other out-of-lab locations.

I-Ming Hsing and colleagues note that PCR works like a biological copy machine, transforming a few wisps of DNA into billions of copies. However, existing PCR machines are so big and complex that they can be used only in laboratories. Scientists have searched for years for a portable, PCR technique that can be used outside the lab.

The study describes a new PCR technique that uses electrochemical DNA sensors to provide simultaneous DNA amplification and detection on a silicon-glass microchip. Their performance tests show that the new technique, called electrochemical real-time PCR (ERT-PCR), is about as fast and sensitive as conventional PCR. The new technique shows "tremendous" promise as a portable system for moving DNA analysis out of the lab and into remote locations, the researchers say.

The article "Electrochemistry-Based Real-Time PCR on a Microchip" is published in the Jan. 15 issue of ACS' Analytical Chemistry.


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The above story is based on materials provided by American Chemical Society. Note: Materials may be edited for content and length.


Cite This Page:

American Chemical Society. "New Microchip For PCR Testing At Crime Scenes, Doctors' Offices." ScienceDaily. ScienceDaily, 24 January 2008. <www.sciencedaily.com/releases/2008/01/080121100909.htm>.
American Chemical Society. (2008, January 24). New Microchip For PCR Testing At Crime Scenes, Doctors' Offices. ScienceDaily. Retrieved October 1, 2014 from www.sciencedaily.com/releases/2008/01/080121100909.htm
American Chemical Society. "New Microchip For PCR Testing At Crime Scenes, Doctors' Offices." ScienceDaily. www.sciencedaily.com/releases/2008/01/080121100909.htm (accessed October 1, 2014).

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