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All Done With Mirrors: Microscope Tracks Nanoparticles In 3-D

Date:
March 13, 2008
Source:
National Institute of Standards and Technology
Summary:
A clever new microscope design allows nanotechnology researchers to track the motions of nanoparticles in solution as they dart around in three dimensions. The researchers hope the technology, which NIST plans to patent, will lead to a better understanding of the dynamics of nanoparticles in fluids and, ultimately, process control techniques to optimize the assembly of nanotech devices.

Heart of the orthogonal tracking microscope system developed at NIST is this nanoparticle solution sample well etched in silicon. Careful orientation of the silicon crystal makes it possible to chemically etch angled sides in the well so smooth they act as mirrors. In this configuration, four side views of a nanoparticle floating in solution (left) are reflected up. A microscope above the well sees the real particle (center, right) and four reflections that show the particle's vertical position.
Credit: NIST

A clever new microscope design allows nanotechnology researchers at the National Institute of Standards and Technology (NIST) to track the motions of nanoparticles in solution as they dart around in three dimensions. The researchers hope the technology, which NIST plans to patent, will lead to a better understanding of the dynamics of nanoparticles in fluids and, ultimately, process control techniques to optimize the assembly of nanotech devices.

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While some nanoscale fabrication techniques borrow from the lithography and solid state methods of the microelectronics industry, an equally promising approach relies on "directed self-assembly." This capitalizes on physical properties and chemical affinities of nanoparticles in solutions to induce them to gather and arrange themselves in desired structures at desired locations. Potential products include extraordinarily sensitive chemical and biological sensor arrays, and new medical and diagnostic materials based on "quantum dots" and other nanoscale materials. But when your product is too small to be seen, monitoring the assembly process is difficult.

Microscopes can help, but a microscope sees a three-dimensional fluid volume as a 2-D plane. There's no real sense of the "up and down" movement of particles in its field of view except that they get more or less fuzzy as they move across the plane where the instrument is in focus. To date, attempts to provide a 3-D view of the movements of nanoparticles in solution largely have relied on that fuzziness. Optics theory and mathematics can estimate how far a particle is above or below the focal plane based on diffraction patterns in the fuzziness. The math, however, is extremely difficult and time consuming and the algorithms are imprecise in practice.

One alternative, NIST researchers reported at the annual meeting of the American Physical Society,* is to use geometry instead of algebra. Specifically, angled side walls of the microscopic sample well act as mirrors to reflect side views of the volume up to the microscope at the same time as the top view. (The typical sample well is 20 microns square and 15 microns deep.)

The microscope sees each particle twice, one image in the horizontal plane and one in the vertical. Because the two planes have one dimension in common, it's a simple calculation to correlate the two and figure out each particle's 3-D path. "Basically, we reduce the problem of tracking in 3-D to the problem of tracking in 2-D twice," explains lead author Matthew McMahon.

The 2-D problem is simpler to solve--several software techniques can calculate and track 2-D position to better than 10 nanometers. Measuring the nanoparticle motion at that fine scale--speeds, diffusion and the like--will allow researchers to calculate the forces acting on the particles and better understand the basic rules of interaction between the various components. That in turn will allow better design and control of nanoparticle assembly processes.

* M. McMahon, A. Berglund, P. Carmichael, J. McClelland and J.A. Liddle. Orthogonal tracking microscopy for nanofabrication research. Paper presented on Monday, March 10, 2008, 1:03 p.m., at the 2008 March Meeting of the American Physical Society, New Orleans, La., March 10-14, 2008.


Story Source:

The above story is based on materials provided by National Institute of Standards and Technology. Note: Materials may be edited for content and length.


Cite This Page:

National Institute of Standards and Technology. "All Done With Mirrors: Microscope Tracks Nanoparticles In 3-D." ScienceDaily. ScienceDaily, 13 March 2008. <www.sciencedaily.com/releases/2008/03/080310131509.htm>.
National Institute of Standards and Technology. (2008, March 13). All Done With Mirrors: Microscope Tracks Nanoparticles In 3-D. ScienceDaily. Retrieved March 5, 2015 from www.sciencedaily.com/releases/2008/03/080310131509.htm
National Institute of Standards and Technology. "All Done With Mirrors: Microscope Tracks Nanoparticles In 3-D." ScienceDaily. www.sciencedaily.com/releases/2008/03/080310131509.htm (accessed March 5, 2015).

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