Featured Research

from universities, journals, and other organizations

New Technique Produces Genetically Identical Stem Cells

Date:
July 6, 2008
Source:
Whitehead Institute for Biomedical Research
Summary:
Cells from mice created using genetically reprogrammed cells can be triggered via drug administration to enter an embryonic-stem-cell-like state without the need for further direct genetic manipulation. This technical advancement enables creation of large numbers of genetically identical cells that can be reprogrammed to an embryonic-stem-cell-like state simply by exposure to a drug. Researchers can exploit such cells to decipher and improve the reprogramming process.

Cells from mice created using genetically reprogrammed cells can be triggered via drug administration (doxycycline) to enter an embryonic-stem-cell-like state without the need for further direct genetic manipulation.
Credit: Tom DiCesare

Adult cells of mice created from genetically reprogrammed cells--so-called induced pluripotent stem (IPS) stem cells--can be triggered via drug to enter an embryonic-stem-cell-like state, without the need for further genetic alteration.

Related Articles


The discovery, which promises to bring new efficiencies to embryonic stem cell research, is reported in Nature Biotechnology.

"This technical advancement will allow thousands of identical reprogrammed cells to be used in experiments," says Marius Wernig, one of the paper's two lead authors and a postdoctoral researcher in Whitehead Member Rudolf Jaenisch's lab.

"Using these cells could help define the milestones of how cells are reprogrammed and screen for drug-like molecules that replace the potentially cancer-causing viruses used for reprogramming," adds Christopher Lengner, the other lead author and also a postdoctoral researcher in the Jaenisch's lab.

In the current work, Wernig and Lengner made mice created in part from the embryonic-stem-cell-like cells known as IPS cells. The IPS cells were created by reprogramming adult skin cells using lentiviruses to randomly insert four genes (Oct4, Sox2, c-Myc and Klf4) into the cells' DNA. The IPS cells also were modified to switch on these four genes when a drug trigger, doxycycline, is added to the cells.

Wernig and Lengner then took cells from each IPS mouse and introduced the doxycycline trigger, thereby changing the adult mouse cells into IPS cells.

While earlier reprogramming experiments have typically induced pluripotency in adult skin cells, Wernig and Lengner were able to employ this novel method to successfully reprogram multiple cell and tissue types, including cells of the intestine, brain, muscle, kidney, adrenal gland, and bone marrow.

Importantly, the technique allows researchers to create large numbers of genetically identical IPS cells, because all cells in the mouse contain the same number of viral integrations in the same location within the genome. With previous approaches, each reprogrammed cell differed because the viruses used to insert the reprogramming genes could integrate anywhere in the cell's DNA with varying frequency.

Wernig and Lengner's method also increases the reprogramming efficiency from one in a thousand cells to one in twenty.

The large numbers of IPS cells that can be created by this method can aid experiments requiring millions of identical cells for reprogramming, such as large-scale chemical library screening assays.

"In experiments, the technique will eliminate many of the reprogramming process's unpredictable variables and simplify enormously the research on the reprogramming mechanism and the screening for virus replacements," says Jaenisch, who is also a professor of biology at Massachusetts Institute of Technology.

The research was supported by the Human Frontiers Science Organization Program, the Ellison Medical Foundation, the Ruth L. Kirschstein National Research Service Award, and the National Institutes of Health.


Story Source:

The above story is based on materials provided by Whitehead Institute for Biomedical Research. Note: Materials may be edited for content and length.


Journal Reference:

  1. Marius Wernig, Christopher J Lengner, Jacob Hanna, Michael Lodato, Eveline Steine, Ruth Foreman, Judith Staerk, Styliani Markoulaki, and Rudolf Jaenisch. A novel drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nature Biotechnology, July 1, 2008

Cite This Page:

Whitehead Institute for Biomedical Research. "New Technique Produces Genetically Identical Stem Cells." ScienceDaily. ScienceDaily, 6 July 2008. <www.sciencedaily.com/releases/2008/07/080701150836.htm>.
Whitehead Institute for Biomedical Research. (2008, July 6). New Technique Produces Genetically Identical Stem Cells. ScienceDaily. Retrieved April 25, 2015 from www.sciencedaily.com/releases/2008/07/080701150836.htm
Whitehead Institute for Biomedical Research. "New Technique Produces Genetically Identical Stem Cells." ScienceDaily. www.sciencedaily.com/releases/2008/07/080701150836.htm (accessed April 25, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, April 25, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

85 Killed in Niger by Meningitis Since Start of Year

85 Killed in Niger by Meningitis Since Start of Year

AFP (Apr. 24, 2015) A meningitis outbreak in Niger has killed 85 people since the start of the year prompting authorities to close schools in the capital Niamey until Monday. Video provided by AFP
Powered by NewsLook.com
C-Section Births a Trend in Brazil

C-Section Births a Trend in Brazil

AFP (Apr. 24, 2015) More than half of Brazil&apos;s babies are born via cesarean section, as mothers and doctors opt for a faster and less painful experience despite the health risks. Duration: 02:02 Video provided by AFP
Powered by NewsLook.com
Anti-Malaria Jab Hope

Anti-Malaria Jab Hope

Reuters - News Video Online (Apr. 24, 2015) The world&apos;s first anti-malaria vaccine could get the go-ahead for use in Africa from October if approved by international regulators. Paul Chapman reports. Video provided by Reuters
Powered by NewsLook.com
3D Food Printing: The Meal of the Future?

3D Food Printing: The Meal of the Future?

AP (Apr. 23, 2015) Developers of 3D food printing hope the culinary technology will revolutionize the way we cook and eat. (April 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins