Featured Research

from universities, journals, and other organizations

Researchers 'All Aglow' Over New Test Of Toxin Strength

Date:
June 20, 2009
Source:
National Institute of Standards and Technology
Summary:
A new NIST assay using a "glow or no glow" technique may soon help the US Department of Homeland Security defend the nation against a spectrum of biological weapons that could be used in a terrorist attack.

Microscopic photographs showing the level of fluorescence (green images on left) and the numbers of cells (blue images on right) at the time the cells were exposed to ricin (top row) and six hours later (bottom row). Note that the fluorescence has decreased (more black regions in the bottom left photo) while the cell count remains virtually the same. This shows that the NIST assay detects ricin even before significant cell death occurs.
Credit: M. Halter, NIST

A new National Institute of Standards and Technology (NIST) assay using a “glow or no glow” technique may soon help the U.S. Department of Homeland Security (DHS) defend the nation against a spectrum of biological weapons that could be used in a terrorist attack. One very dangerous toxin on the list is ricin, a protein derived from castor beans that is lethal in doses as small as 500 micrograms—about the size of a grain of salt.

Related Articles


As part of its efforts to address the threat, DHS is working with NIST to create a standardized ricin sample with a known potency. Such a standard is needed both to check the accuracy of detection equipment, and, should an attack occur, to confirm the success of decontamination procedures. A major step toward this goal—the development of a rapid, reliable and precise assay of the potency of a ricin sample—has now been achieved by NIST scientists.

As detailed in an article posted online this week in Assay and Drug Development Technologies, the new NIST assay uses a commercially available cell line genetically engineered to produce large amounts of green fluorescent protein (GFP). Ricin shuts down ribosomes—the protein manufacturing factories of a cell. Assay cells exposed to the toxin will quickly stop synthesizing GFP. This, in turn, will result in a measurable decrease in fluorescence—a drop that correlates directly to the strength of the ricin present.

The NIST assay yields many advantages over traditional cytotoxicity measuring systems, including: a highly sensitive response to ricin (as little as 1 nanogram per milliliter) within six rather than 24 hours; detection of the toxin long before significant cell death has occurred; results that are highly reproducible; no need for the addition of any reagents; and the flexibility to measure the potency of other ribosome inhibitors, even nanoparticles, with the same precision as ricin.

Partial support for this work was provided by the DHS Science and Technology Directorate.


Story Source:

The above story is based on materials provided by National Institute of Standards and Technology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Halter et al. A Mechanistically Relevant Cytotoxicity Assay Based on the Detection of Cellular Green Fluorescent Protein. Assay and Drug Development Technologies, 2009; 090616052622098 DOI: 10.1089/adt.2009.192

Cite This Page:

National Institute of Standards and Technology. "Researchers 'All Aglow' Over New Test Of Toxin Strength." ScienceDaily. ScienceDaily, 20 June 2009. <www.sciencedaily.com/releases/2009/06/090617105052.htm>.
National Institute of Standards and Technology. (2009, June 20). Researchers 'All Aglow' Over New Test Of Toxin Strength. ScienceDaily. Retrieved January 28, 2015 from www.sciencedaily.com/releases/2009/06/090617105052.htm
National Institute of Standards and Technology. "Researchers 'All Aglow' Over New Test Of Toxin Strength." ScienceDaily. www.sciencedaily.com/releases/2009/06/090617105052.htm (accessed January 28, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Wednesday, January 28, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Aquaponics Turn Suburban Industrial Park Into Farmland: Hume

Aquaponics Turn Suburban Industrial Park Into Farmland: Hume

The Toronto Star (Jan. 27, 2015) Ancient techniques of growing greens with fish and water are well ahead of Toronto bylaws. Video provided by The Toronto Star
Powered by NewsLook.com
Chihuahua Sleeps on Top of Great Dane

Chihuahua Sleeps on Top of Great Dane

Rumble (Jan. 27, 2015) As this giant Great Dane lays down for bedtime he accompanied by an adorable companion. Watch a tiny Chihuahua jump up and prepare to sleep on top of his friend. Now that&apos;s a pretty big bed! Credit to &apos;emma_hussey01&apos;. Video provided by Rumble
Powered by NewsLook.com
Madagascar Locust Plague Could Mean Famine For Millions

Madagascar Locust Plague Could Mean Famine For Millions

Newsy (Jan. 27, 2015) The Food and Agriculture Organization says millions could face famine in Madagascar without more funding to finish locust eradication efforts. Video provided by Newsy
Powered by NewsLook.com
Shark Bite Victim Making Amazing Recovery

Shark Bite Victim Making Amazing Recovery

AP (Jan. 27, 2015) A Texas woman who lost more than five pounds of flesh to a shark in the Bahamas earlier this month could be released from a Florida hospital soon. Experts believe she was bitten by a bull shark while snorkeling. (Jan. 27) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins