Featured Research

from universities, journals, and other organizations

GEN reports on enhancing the applications of qPCR

Date:
November 18, 2009
Source:
Mary Ann Liebert, Inc./Genetic Engineering News
Summary:
Quantitative polymerase chain reaction (qPCR) technology is experiencing a surge of interest and rapid expansion as a result of advances such as instrumentation that pushes capacity to 1,536 wells and optimization-free multiplexing. The technique's ability to both detect and simultaneously quantify specific DNA sequences is increasing its use in basic research and diagnostics, according to a new study.

Quantitative polymerase chain reaction (qPCR) technology is experiencing a surge of interest and rapid expansion as a result of advances such as instrumentation that pushes capacity to 1,536 wells and optimization-free multiplexing, reports Genetic Engineering & Biotechnology News (GEN). The technique's ability to both detect and simultaneously quantify specific DNA sequences is increasing its use in basic research and diagnostics, according to the November 15 issue of GEN.

"With some estimates of the qPCR assay consumables market totaling over three-quarters of a billion dollars, and growing, it's obvious that this particular technology has found a solid niche in life science research," says John Sterling, Editor in Chief of GEN.

A number of companies are making important improvements to qPCR methodologies. For example, Qiagen has developed a number of technologies for multiplex PCR that eliminate the need for optimization. Its multiplex PCR technology uses a highly stringent hot-start enzyme, an ammonium-containing buffer, and a multiplex PCR-enhancing synthetic factor MP that reportedly eliminates the need for optimization because it supports macromolecular crowding. Thus it allows efficient annealing of multiple primers under identical cycling conditions.

Bio-Rad Laboratories recently introduced its iScript™ RT-qPCR sample-preparation reagent for isolation of total RNA and for enabling reverse transcription and real-time PCR to be performed directly from cell lysates. Company officials say that scientists can use the protocol to remove genomic DNA and stabilize RNA in as little as five minutes.


Story Source:

The above story is based on materials provided by Mary Ann Liebert, Inc./Genetic Engineering News. Note: Materials may be edited for content and length.


Cite This Page:

Mary Ann Liebert, Inc./Genetic Engineering News. "GEN reports on enhancing the applications of qPCR." ScienceDaily. ScienceDaily, 18 November 2009. <www.sciencedaily.com/releases/2009/11/091118110700.htm>.
Mary Ann Liebert, Inc./Genetic Engineering News. (2009, November 18). GEN reports on enhancing the applications of qPCR. ScienceDaily. Retrieved August 20, 2014 from www.sciencedaily.com/releases/2009/11/091118110700.htm
Mary Ann Liebert, Inc./Genetic Engineering News. "GEN reports on enhancing the applications of qPCR." ScienceDaily. www.sciencedaily.com/releases/2009/11/091118110700.htm (accessed August 20, 2014).

Share This




More Health & Medicine News

Wednesday, August 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Possible Ebola Patient in Isolation at California Hospital

Possible Ebola Patient in Isolation at California Hospital

Reuters - US Online Video (Aug. 20, 2014) — A patient who may have been exposed to the Ebola virus is in isolation at the Kaiser Permanente South Sacramento Medical Center. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Raw: World's Oldest Man Lives in Japan

Raw: World's Oldest Man Lives in Japan

AP (Aug. 20, 2014) — A 111-year-old Japanese was certified as the world's oldest man by Guinness World Records on Wednesday. Sakari Momoi, a native of Fukushima in northern Japan, was given a certificate at a hospital in Tokyo. (Aug. 20) Video provided by AP
Powered by NewsLook.com
Do More Wedding Guests Make A Happier Marriage?

Do More Wedding Guests Make A Happier Marriage?

Newsy (Aug. 20, 2014) — A new study found couples who had at least 150 guests at their weddings were more likely to report being happy in their marriages. Video provided by Newsy
Powered by NewsLook.com
Ebola-Hit Sierra Leone's Freetown a City on Edge

Ebola-Hit Sierra Leone's Freetown a City on Edge

AFP (Aug. 19, 2014) — Residents of Sierra Leone's capital voice their fears as the Ebola virus sweeps through west Africa. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins