Featured Research

from universities, journals, and other organizations

'Digital embryo' gains wings: Now possible to film development of fruit fly and of zebrafish's eyes and brain

Date:
July 6, 2010
Source:
European Molecular Biology Laboratory
Summary:
Scientists in Europe have captured fruit fly development on film, creating the Fly Digital Embryo. In a newly published study, they were also the first to clearly record how a zebrafish's eyes and mid-brain are formed.

The fly digital embryo is shown here at different developmental stages, with cell nuclei colored according to how fast they were moving (from blue for the slowest to orange for the fastest). The fruit fly embryo is magnified around 250 times.
Credit: Philipp Keller/EMBL

Scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, who 'fathered' the Digital Embryo have now given it wings, creating the Fly Digital Embryo.

Related Articles


In a study published in Nature Methods, they describe how they were able to capture fruit fly development on film, and were the first to clearly record how a zebrafish's eyes and midbrain are formed. The improved technique will also help to shed light on processes and organisms, which have so far been under-studied because they could not be followed under a microscope.

"Non-transparent samples like the fruit fly embryo scatter light, so the microscope picks up a mixture of in-focus and out-of-focus signal- good and bad information, if you like," says Ernst Stelzer, whose group carried out the project at EMBL. "Our new technique enables us to discriminate between that good and bad information, so it allows us to record organisms which have so far been poorly studied, because of their unfortunate optical properties."

Philipp Keller, who co-led and conducted the work, and Ernst Stelzer overcame the difficulties caused by thick, opaque samples, by shining patterns of light on them, instead of the usual continuous light sheet. This generates an image with alternating light and dark stripes, unless the light bounces off the sample and changes direction, in which case this stripy pattern will be blurred. By taking multiple images of different phases of the light pattern, and combining them, a computer can filter out the effects of scattered light and generate an accurate image of the sample, thus enabling scientists to record images that were previously unobtainable.

By combining this approach with imaging along different angles, the scientists were able to obtain three-dimensional movies of the developing fruit fly embryo in spite of the fact that it is almost opaque.

The EMBL scientists were also able to extend their recordings of zebrafish development to an unprecedented level. They took around one million images to capture the first three days of zebrafish development from three different angles, generating films in which the formation of the animal's eyes and midbrain are clearly visible.

"Of course, getting such good images is nice for the human observer, but it's particularly crucial for computational analyses, like tracking cell movements and divisions as we do in the Digital Embryo," says Philipp Keller, now at the Janelia Farm Research Campus of the Howard Hughes Medical Institute in Ashburn, VA, USA.

The work was done in collaboration with scientists at the University of Heidelberg, Germany and the Sloan-Kettering Institute in New York, USA. All data, images and videos are freely available online, alongside the data from the digital embryo, at www.digital-embryo.org.


Story Source:

The above story is based on materials provided by European Molecular Biology Laboratory. Note: Materials may be edited for content and length.


Cite This Page:

European Molecular Biology Laboratory. "'Digital embryo' gains wings: Now possible to film development of fruit fly and of zebrafish's eyes and brain." ScienceDaily. ScienceDaily, 6 July 2010. <www.sciencedaily.com/releases/2010/07/100705073919.htm>.
European Molecular Biology Laboratory. (2010, July 6). 'Digital embryo' gains wings: Now possible to film development of fruit fly and of zebrafish's eyes and brain. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2010/07/100705073919.htm
European Molecular Biology Laboratory. "'Digital embryo' gains wings: Now possible to film development of fruit fly and of zebrafish's eyes and brain." ScienceDaily. www.sciencedaily.com/releases/2010/07/100705073919.htm (accessed December 18, 2014).

Share This


More From ScienceDaily



More Computers & Math News

Thursday, December 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Jaguar Unveils 360 Virtual Windshield Making Car Pillars Appear Transparent

Jaguar Unveils 360 Virtual Windshield Making Car Pillars Appear Transparent

Buzz60 (Dec. 17, 2014) Jaguar unveils a virtual 360 degree windshield that may be the most futuristic automotive development yet. Jen Markham explains. Video provided by Buzz60
Powered by NewsLook.com
BlackBerry Launches Classic Smartphone

BlackBerry Launches Classic Smartphone

AP (Dec. 17, 2014) BlackBerry is returning to its roots with a new smartphone called the Classic, featuring a traditional keyboard at a time when rival Apple and Android phones - and most smartphone customers - have embraced touch screens. (Dec. 17) Video provided by AP
Powered by NewsLook.com
The Future of Work, Skills & Careers in a Digital World-Dr. Tracy Wilen

The Future of Work, Skills & Careers in a Digital World-Dr. Tracy Wilen

Working Mother (Dec. 16, 2014) 2014 Worklife Congress Video provided by Working Mother
Powered by NewsLook.com
Tech Companies Make Holiday Shopping Easier Than Ever

Tech Companies Make Holiday Shopping Easier Than Ever

Newsy (Dec. 16, 2014) Innovative new services allow consumers to shop with their smartphones, split bills and even haggle. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins