Featured Research

from universities, journals, and other organizations

Multicolor quantum dots aid in cancer biopsy diagnosis

Date:
July 7, 2010
Source:
Emory University
Summary:
The tunable fluorescent nanoparticles known as quantum dots make ideal tools for distinguishing and identifying rare cancer cells in tissue biopsies. Researchers have learned how multicolor quantum dots linked to antibodies can distinguish the Reed-Sternberg cells that are characteristic of Hodgkin's lymphoma.

Reed-Sternberg cells can be distinguished by their red outline, blue and white internal staining, and their lack of green staining.
Credit: Shuming Nie

The tunable fluorescent nanoparticles known as quantum dots make ideal tools for distinguishing and identifying rare cancer cells in tissue biopsies, Emory and Georgia Tech scientists have demonstrated.

An article to be featured on the cover of the July 15 issue of Analytical Chemistry describes how multicolor quantum dots linked to antibodies can distinguish the Reed-Sternberg cells that are characteristic of Hodgkin's lymphoma.

"Our multicolor quantum dot staining method provides rapid detection and identification of rare malignant cells from heterogenous tissue specimens," says senior author Shuming Nie, PhD, the Wallace H. Coulter distinguished professor in the Coulter department of biomedical engineering at Georgia Tech and Emory University. "The clinical utility is not limited to Hodgkin's lymphoma but potentially could be extended to detect cancer stem cells, tumor-associated macrophages and other rare cell types."

Quantum dots are nanometer-sized semiconductor crystals that have unique chemical and physical properties due to their size and their highly compact structure. Quantum dots can be chemically linked to antibodies, which can detect molecules present on the surfaces or internal parts of cancer cells.

As a test of quantum dots' discriminatory power, the authors used four varieties at once -- white, red, green and blue -- each detecting a different protein, to stain lymph node biopsies. The goal was to distinguish six Hodgkin's lymphoma cases from two other types of lymphoma and samples from two patients with benign growths in their lymph nodes.

Reed Sternberg cells have a distinctive appearance, but in lymph node tissue, they are usually surrounded by other white blood cells. The authors describe identifying them as a task like "finding a needle in a haystack."

"We're excited about this technology," says Andrew Young, MD, PhD, associate professor of pathology and laboratory medicine at Emory University School of Medicine and director of clinical laboratories at Grady Health System. "We expect it could help guide the type of treatment a cancer patient gets and that it could be used with a wider variety of tumor types."

The most reliable way to assign cell identity is to look at more than one protein, Young says. With the standard methods in most pathology labs, staining cells with four different antibodies would require four separate slides -- a problem when the specimen is very small. Small diagnostic specimens are common today, because they minimize the burden on the patient. In addition, the images from multiple separate slides wouldn't depict exactly the same cells. The quantum dots allow "multiplexing": superimposing four colors on top of each other.

Hodgkin's lymphoma is usually treated with chemotherapy and radiation, and is notable among the subtypes of adult lymphoma because the survival rate is relatively high. Young says the quantum dot technique could be useful for other types of cancer, where distinguishing cancer cells based on surface or genetic markers can point oncologists towards "targeted therapies" designed for one particular type of tumor.

Shuming Nie is director and principal investigator of Emory's Center for Cancer Nanotechnology Excellence, supported by the National Cancer Institute. He is associate director for nanotechnology bioengineering at the Winship Cancer Institute of Emory University and a Georgia Cancer Coalition Scholar.

The research was supported by the National Cancer Institute.


Story Source:

The above story is based on materials provided by Emory University. The original article was written by Quinn Eastman. Note: Materials may be edited for content and length.


Journal Reference:

  1. Jian Liu, Stephen K. Lau, Vijay A. Varma, Brad A. Kairdolf, Shuming Nie. Multiplexed Detection and Characterization of Rare Tumor Cells in Hodgkin%u2019s Lymphoma with Multicolor Quantum Dots. Analytical Chemistry, 2010; 100621135249023 DOI: 10.1021/ac101065b

Cite This Page:

Emory University. "Multicolor quantum dots aid in cancer biopsy diagnosis." ScienceDaily. ScienceDaily, 7 July 2010. <www.sciencedaily.com/releases/2010/07/100706150624.htm>.
Emory University. (2010, July 7). Multicolor quantum dots aid in cancer biopsy diagnosis. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2010/07/100706150624.htm
Emory University. "Multicolor quantum dots aid in cancer biopsy diagnosis." ScienceDaily. www.sciencedaily.com/releases/2010/07/100706150624.htm (accessed October 20, 2014).

Share This



More Health & Medicine News

Monday, October 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Microneedle Patch Promises Painless Pricks

Microneedle Patch Promises Painless Pricks

Reuters - Innovations Video Online (Oct. 18, 2014) Researchers at The National University of Singapore have invented a new microneedle patch that could offer a faster and less painful delivery of drugs such as insulin and painkillers. Video provided by Reuters
Powered by NewsLook.com
Raw: Nurse Nina Pham Arrives in Maryland

Raw: Nurse Nina Pham Arrives in Maryland

AP (Oct. 17, 2014) The first nurse to be diagnosed with Ebola at a Dallas hospital walked down the stairs of an executive jet into an ambulance at an airport in Frederick, Maryland, on Thursday. Pham will be treated at the National Institutes of Health. (Oct. 16) Video provided by AP
Powered by NewsLook.com
Raw: Cruise Ship Returns to US Over Ebola Fears

Raw: Cruise Ship Returns to US Over Ebola Fears

AP (Oct. 17, 2014) A Caribbean cruise ship carrying a Dallas health care worker who is being monitored for signs of the Ebola virus is heading back to Texas, US, after being refused permission to dock in Cozumel, Mexico. (Oct. 17) Video provided by AP
Powered by NewsLook.com
Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

Spanish Govt: Four Suspected Ebola Cases in Spain Test Negative

AFP (Oct. 17, 2014) All four suspected Ebola cases admitted to hospitals in Spain on Thursday have tested negative for the deadly virus in a first round of tests, the government said Friday. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins